Partially Acid-Hydrolyzed Porphyran Improved Dextran Sulfate Sodium-Induced Acute Colitis by Modulation of Gut Microbiota and Enhancing the Mucosal Barrier

Abstract

The pharmacological values of marine algal polysaccharides on gut health are being recognized in recent research. However, the protective effect of degraded polysaccharides from Porphyra haitanensis (PHP-D) on the colonic mucosal barrier damaged in ulcerative colitis is poorly understood. The purpose of this study was to investigate how PHP-D could maintain the integrity of colonic mucosal layer mediated by microbiota in a dextran sulfate sodium (DSS)-induced colitis mouse model. Structural analysis revealed that PHP-D had a typical porphyran structure having a backbone of alternating (1 → 3)-linked β-D-galactopyranose units linked to either (1 → 4)-3,6-anhydro-α-L-galactopyranose units or (1 → 4)-linked α-L-galactose-6-sulfate units. An in vivo study demonstrated that PHP-D treatment reduced the severity of DSS-induced ulcerative colitis. 16S rRNA phylogenetic sequencing revealed that PHP-D affected the diversity of gut microbiota with an increase of Bacteroides, Muribaculum, and Lactobacillus species. Similarly, PHP-D increased levels of short-chain fatty acids. Furthermore, PHP-D restored mucus thickness and improved the expression of tight junction proteins. This work demonstrates that PHP-D is capable of enhancing a colonic mucosal barrier. These outcomes offer unique perspectives on the potential application of P. haitanensis as a promising natural product for the management of ulcerative colitis.

Polysaccharides from brown seaweed: Physicochemical properties, absorption in the intestine, and beneficial effects on intestinal barrier

“…The intestinal barrier maintains a peaceful coexistence with symbionts and protects the host against infection and diseases related to intestinal function disorder (Chen et al, 2023). Growing studies focus on the interactions between dietary polysaccharides and intestinal barriers (Yu et al, 2023). Table 1 illustrates the beneficial effects of BSP on the intestinal barrier, presenting the polysaccharide, source, trial stage, and impact on the intestinal epithelium and microbiota.…”

Comparative Study on Anti-Inflammatory Effect of Polysaccharides from Vinegar-Baked Radix Bupleuri Using Different Methods

“…As a newly developing area of natural products research, the varied activities of polysaccharides have attracted increasing attention. Unlike small molecules, the structure and activities of polysaccharides are greatly affected by the extraction method. − However, a few reports have focused on the extraction process, and the results seem to depend on the herb and the activity which is studied …”

“…Polysaccharides from vinegar-baked Radix Bupleuri (VBCP) have a much larger molecular weight (Mw) than polysaccharides from the original herb, increasing the solubility of baicalin and enhancing the anti-virus effect of oxymatrine. , However, it is unknown whether VBCP has anti-inflammatory effects, which are one of the main causes of liver diseases. Moreover, different methods may induce changes in the characteristics and activity of polysaccharides, − and which method produces the highest yield and the most powerful anti-inflammatory effect is not clear.…”

Preparation, Characterization, and Evaluation of Physcion Nanoparticles for Enhanced Oral Bioavailability: An Attempt to Improve Its Antioxidant and Anticancer Potential

“…Subsequently, they remove electrons from other molecules and thus not only affect cell regulation but also damage molecules including DNA, proteins, and carbohydrates. Cell damage, especially the damage to DNA, may cause the development of cancer. , Antioxidants are capable of stabilizing reactive oxygen species (ROS) by donating electrons and thus inhibit their detrimental effects. Cancer is a complex, heterogeneous disease with approx.…”