Partially hydrolyzed guar gum down-regulates colonic inflammatory response in dextran sulfate sodium-induced colitis in mice

Naito, Yuji; Takagi, Tomohisa; Katada, Kazuhiro; Uchiyama, Kazuhiko; Kuroda, Masaaki; Kokura, Satoshi; Ichikawa, Hiroshi; Watabe, Junko; Yoshida, Norimasa; Okanoue, Takeshi; Yoshikawa, Toshikazu

doi:10.1016/j.jnutbio.2005.08.010

Cited by 60 publications

(52 citation statements)

References 43 publications

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“…We showed that MPO activity was enhanced in DSS-induced pouchitis and that this increase was reversed by PHGG treatment. It has been shown that neutrophil-mediated inflammation is one of the main pathways in the development of DSS-induced colonic mucosal injury [32][33][34][35]. The present study has shown that MPO activity, an index of tissue-associated neutrophil accumulation, significantly increases in ileal mucosa after J pouch configuration and DSS administration, and that this increase was significantly inhibited by treatment with PHGG.…”

Section: Discussionsupporting

confidence: 58%

Partially Hydrolyzed Guar Gum Attenuates the Severity of Pouchitis in a Rat Model of Ileal J Pouch-Anal Anastomosis

et al. 2008

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We aimed to evaluate the efficacy of treatment with partially hydrolyzed guar gum (PHGG) using a rat model of ileal pouch-anal anastomosis and pouchitis. In the J pouch groups, tissue myeloperoxidase activities were significantly higher than native myeloperoxidase activities (P = 0.020; P = 0.015; P = 0.004, respectively). A statistically significant difference in total histological score was detected in the J pouch + 5% dextran sulfate sodium (DSS) group, compared to the J pouch control and the J pouch + 5% DSS + PHGG groups (P < 0.01 and P < 0.01, respectively). There was a significant overgrowth of aerobes and anaerobes in the J pouch + 5% DSS group. This study demonstrated that rectal administration of PHGG attenuates the severity of pouchitis in a rat model. In conclusion, PHGG may be an additional therapeutic strategy for the treatment of pouchitis.

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Section: Discussionsupporting

confidence: 58%

Partially Hydrolyzed Guar Gum Attenuates the Severity of Pouchitis in a Rat Model of Ileal J Pouch-Anal Anastomosis

et al. 2008

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“…boulardii. To our knowledge, few data suggest direct modulation of proinflammatory gene expression by mannan polysaccharides (25). Nevertheless, their anti-inflammatory effects are mainly related to the prevention of pathogen adhesion and invasion, as discussed below.…”

Section: Discussionmentioning

confidence: 99%

β-Galactomannan and Saccharomyces cerevisiae var. boulardii Modulate the Immune Response against Salmonella enterica Serovar Typhimurium in Porcine Intestinal Epithelial and Dendritic Cells

Badía¹,

Brufau²,

Guerrero-Zamora³

et al. 2012

Clin. Vaccine Immunol.

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Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen that causes inflammation, necrosis, and diarrhea in pigs, as well as being an important source of food-borne diseases in humans. Probiotics and prebiotics are promising alternatives to antibiotics to control and prevent intestinal infections. The present work investigated a recently developed ␤-galactomannan (␤GM) prebiotic compared to the proven probiotic Saccharomyces cerevisiae var. boulardii on porcine ileum intestinal epithelial cells (IECs) of the IPI-2I line and monocyte-derived dendritic cells (DCs) cocultured in vitro with Salmonella. We observed that both S. cerevisiae var. boulardii and ␤GM inhibited the association of Salmonella with IECs in vitro. Our data indicated that ␤GM has a higher ability than S. cerevisiae var. boulardii to inhibit Salmonella-induced proinflammatory mRNA (cytokines tumor necrosis factor alpha [TNF-␣], interleukin-1␣ [IL-1␣], IL-6, and granulocyte-macrophage colony-stimulating factor [GM-CSF] and chemokines CCL2, CCL20, and CXCL8) and at protein levels (IL-6 and CXCL8). Additionally, ␤GM and S. cerevisiae var. boulardii induced some effects on DCs that were not observed on IECs: ␤GM and S. cerevisiae var. boulardii showed slight upregulation of mRNA for TNF-␣, GM-CSF, and CCR7 receptor on porcine monocyte-derived dendritic cells (DCs). Indeed, the addition of ␤GM or S. cerevisiae var. boulardii on DCs cocultured with Salmonella showed higher gene expression (mRNA) for TNF-␣, GM-CSF, and CXCL8 compared to that of the control with Salmonella. In conclusion, the addition of ␤GM inhibits Salmonella-induced proinflammatory profiles in IECs but may promote DC activation, although associated molecular mechanisms remain to be elucidated.

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“…The parameters recorded in the experiments included the disease activity index (DAI), colon length, and histology. The DAI was determined by scoring changes in weight, occult blood positivity, gross bleeding, and stool consistency, as described previously (Table I) (17)(18)(19). Occult bleeding was tested using a commercial kit based on the detection of the peroxidase activity of heme in the stool (Occult Blood Slide 5 Shionogi; Shionogi & Co., Osaka, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…The DAI is a mean of individual scores of weight loss, stool consistency, and bleeding (17)(18)(19). Normal stools, formed pellets; loose stools, pasty and semiformed stools that do not stick to the anus; diarrhea, liquid stools that stick to the anus.…”

Section: Measurement Of Mpo Activity and Tba-reactive Substancesmentioning

confidence: 99%

Rosuvastatin, a new HMG-CoA reductase inhibitor, reduces the colonic inflammatory response in dextran sulfate sodium-induced colitis in mice

Naito¹,

Katada²,

Takagi³

et al. 2006

Int J Mol Med

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Abstract. The aim of the present study was to elucidate the beneficial effects of rosuvastatin, a new HMG-CoA reductase inhibitor, on colonic mucosal damage and on the inflammatory response in a dextran sulfate sodium (DSS) colitis model. Acute colitis was induced using 8% DSS in female BALB/c mice. Colonic mucosal inflammation was evaluated clinically, biochemically, and histologically. Mucosal protein contents and mRNA levels of tumor necrosis factor (TNF)-· were determined by immunoassay and real time-PCR. The mRNA levels of endothelial nitric oxide synthase (eNOS) were determined by real-time PCR. Disease activity scores in DSSinduced colitis model mice, as determined by weight loss, stool consistency, and blood in stool, were significantly lower in the rosuvastatin-treated mice than in control mice. Shortening of the colon was significantly reversed by rosuvastatin. Increases in tissue-associated myeloperoxidase activity and thiobarbituric acid-reactive substances after DSS administration were both significantly inhibited by treatment with rosuvastatin. Rosuvastatin also inhibited increases in intestinal TNF-· protein and mRNA expression after DSS administration, respectively. The mucosal mRNA levels of eNOS were decreased after DSS administration, but preserved in mice treated with rosuvastatin. These results suggest that rosuvastatin prevents the development of DSS-induced colitis in mice via the inhibition of mucosal inflammatory responses associated with the preservation of eNOS transcription.

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Partially hydrolyzed guar gum down-regulates colonic inflammatory response in dextran sulfate sodium-induced colitis in mice

Cited by 60 publications

References 43 publications

Partially Hydrolyzed Guar Gum Attenuates the Severity of Pouchitis in a Rat Model of Ileal J Pouch-Anal Anastomosis

Partially Hydrolyzed Guar Gum Attenuates the Severity of Pouchitis in a Rat Model of Ileal J Pouch-Anal Anastomosis

β-Galactomannan and Saccharomyces cerevisiae var. boulardii Modulate the Immune Response against Salmonella enterica Serovar Typhimurium in Porcine Intestinal Epithelial and Dendritic Cells

Rosuvastatin, a new HMG-CoA reductase inhibitor, reduces the colonic inflammatory response in dextran sulfate sodium-induced colitis in mice

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