Partially PEGylated dendrimer-entrapped gold nanoparticles: a promising nanoplatform for highly efficient DNA and siRNA delivery

Hou, Wenxiu; Wei, Ping; Kong, Lingdan; Guo, Rui; Wang, Shige; Shi, Xiangyang

doi:10.1039/c6tb00710d

Cited by 63 publications

(70 citation statements)

References 41 publications

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“…Due to their unique properties, GNP are used more and more in the biomedical field, especially for drug delivery, gene delivery, and thermal treatment of cancer . GNP are usually synthesized from HAuCl 4 , a gold salt that can be reduced by various methods, such as Brust or Turkevish ones.…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticles in ophthalmology

Masse

Ouellette

Lamoureux

et al. 2018

Medicinal Research Reviews

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Many research projects are underway to improve the diagnosis and therapy in ophthalmology. Indeed, visual acuity deficits affect 285 million people worldwide and different strategies are being developed to strengthen patient care. One of these strategies is the use of gold nanoparticles (GNP) for their multiple properties and their ability to be used as both diagnosis and therapy tools. This review exhaustively details research developing GNPs for use in ophthalmology. The toxicity of GNPs and their distribution in the eye are described through in vitro and in vivo studies. All publications addressing the pharmacokinetics of GNPs administered in the eye are extensively reviewed. In addition, their use as biosensors or for imaging with optical coherence tomography is illustrated. The future of GNPs for ophthalmic therapy is also discussed. GNPs can be used to deliver genes or drugs through different administration routes. Their antiangiogenic and anti-inflammatory properties are of great interest for different ocular pathologies. Finally, GNPs can be used to improve stereotactic radiosurgery, brachytherapy, and photothermal therapy because of their many properties.

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Section: Introductionmentioning

confidence: 99%

Gold nanoparticles in ophthalmology

Masse

Ouellette

Lamoureux

et al. 2018

Medicinal Research Reviews

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“…Although the AuNP‐linked plasmid deoxyribonucleic acid (DNA) or short interfering ribonucleic acid (siRNA) had been proved to facilitate their intracellular delivery, recent endeavors revealed that the polymer–Au hybrid nanoparticles with biocompatible polymer modification could further improve the stability of AuNP gene carriers, reduce the toxicity of carrier, and enhance gene delivery efficiency, as exampled in Figure a . As a typical example, Shi and co‐workers designed generation 5 (G5) dendrimer‐entrapped AuNPs with partially surface modification by PEG, and achieved improved expression efficiency of Luc or enhanced green fluorescent protein (EGFP) plasmid DNA as well as enhanced silencing effect by delivering Bcl‐2 siRNA . By capping AuNPs with l ‐cysteine methyl ester hydrochloride‐modified PEG, Rahme et al synthesized highly stable polymer–Au hybrid NPs, thanks to PEGylation‐induced interaction inhibition with serum proteins to avoid aggregation or to protect gene degradation by nucleases, as shown in Figure b .…”

Section: Applicationmentioning

confidence: 99%

Recent Progress in Macromolecule‐Anchored Hybrid Gold Nanomaterials for Biomedical Applications

Luo

et al. 2018

Macromol. Rapid Commun.

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Gold nanoparticles (AuNPs), with elegant thermal, optical, or chemical properties due to quantum size effects, may serve as unique species for therapeutic or diagnostic applications. It is worth mentioning that their small size also results in high surface activity, leading to significantly impaired stability, which greatly hinders their biomedical utilizations. To overcome this problem, various types of macromolecular materials are utilized to anchor AuNPs so as to achieve advanced synergistic effect by dispersing, protecting, and stabilizing the AuNPs in polymeric-Au hybrid self-assemblies. In this review, the most recent development of polymer-AuNP hybrid systems, including AuNPs@polymeric nanoparticles, AuNPs@polymeric micelle, AuNPs@polymeric film, and AuNPs@polymeric hydrogel are discussed with respect to their different synthetic strategies. These sophisticated materials with diverse functions, oriented toward biomedical applications, are further summarized into several active domains in the areas of drug delivery, gene delivery, photothermal therapy, antibacterials, bioimaging, etc. Finally, the possible approaches for future design of multifunctional polymer-AuNP hybrids by combining hybrid chemistry with biological interface science are proposed.

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“…Several types of NPs, such as liposomes, polymers, dendrimers, metal (e.g., gold, silver, iron oxide), and carbon, have being developed for application in cancer therapeutics [10]. Interestingly, a combination of different NPs, dendrimer-entrapped gold nanoparticles were recently used as a nanoplatform to induce gene silencing of cancer cells [22,23]. …”

Section: Addressing Current Drawbacks In Gene Silencing Therapy—fomentioning

confidence: 99%

“…As described above, dendrimer-entrapped gold nanoparticles (Au DENPs) were successfully used as platform for the delivery of vascular endothelial growth factor ( VEGF ) or B-cell lymphoma/leukemia 2 protein ( BCL-2 ) siRNA into a human glioma cell line [22] or BCL-2 siRNA to a human cervical carcinoma cell line, for silencing of luciferase ( LUC ) reporter gene and enhanced green fluorescent protein ( EGFP ) gene [23]. Moreover, Cui et al investigated dendrimer-coated AuNRs for brcaa1-shRNA delivery into MCF7 cancer cells, which led to a successful silencing of breast cancer 1 ( BRCAA1 ) gene [44].…”

Section: Addressing Current Drawbacks In Gene Silencing Therapy—fomentioning

confidence: 99%

Gold Nanoparticle Approach to the Selective Delivery of Gene Silencing in Cancer—The Case for Combined Delivery?

Mendes

Fernandes

Baptista

2017

Genes

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Gene therapy arises as a great promise for cancer therapeutics due to its potential to silence genes involved in tumor development. In fact, there are some pivotal gene drivers that suffer critical alterations leading to cell transformation and ultimately to tumor growth. In this vein, gene silencing has been proposed as an active tool to selectively silence these molecular triggers of cancer, thus improving treatment. However, naked nucleic acid (DNA/RNA) sequences are reported to have a short lifetime in the body, promptly degraded by circulating enzymes, which in turn speed up elimination and decrease the therapeutic potential of these drugs. The use of nanoparticles for the effective delivery of these silencers to the specific target locations has allowed researchers to overcome this issue. Particularly, gold nanoparticles (AuNPs) have been used as attractive vehicles for the target-specific delivery of gene-silencing moieties, alone or in combination with other drugs. We shall discuss current trends in AuNP-based delivery of gene-silencing tools, considering the promising road ahead without overlooking existing concerns for their translation to clinics.

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Partially PEGylated dendrimer-entrapped gold nanoparticles: a promising nanoplatform for highly efficient DNA and siRNA delivery

Abstract: Partially PEGylated dendrimer-entrapped gold nanoparticles can be used as a promising nanoplatform for highly efficient pDNA and siRNA delivery.

Cited by 63 publications

References 41 publications

Gold nanoparticles in ophthalmology

Gold nanoparticles in ophthalmology

Recent Progress in Macromolecule‐Anchored Hybrid Gold Nanomaterials for Biomedical Applications

Gold Nanoparticle Approach to the Selective Delivery of Gene Silencing in Cancer—The Case for Combined Delivery?

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