Participation of a sialic acid-specific lectin from freshwater prawnMacrobrachium rosenbergii hemocytes in the recognition of non-self cells

Vázquez, Lorena; Maldonado, Gabriela Inés; Agundis, Concepción; Pérez, A; Cooper, Edwin L.; Zenteno, Edgar

doi:10.1002/(sici)1097-010x(19971015)279:3<265::aid-jez7>3.0.co;2-l

Cited by 43 publications

(30 citation statements)

References 25 publications

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“…Among the compounds tested, only NANA and the sialoglycoproteins inhibited the lectin activity. Sialic acids, which are often found as terminal sugars of glycoconjugates, play an important role in many biological mechanisms (17,33,34,43,44). In a previous report, it was demonstrated that NANA and sialyl lactose inhibited the binding of laminin and fibrinogen to A. fumigatus conidia (3), suggesting an interaction mediated by a sialic acid-specific lectin of the conidial wall.…”

Section: Discussionmentioning

confidence: 99%

Purification and Partial Characterization of a 32-Kilodalton Sialic Acid-Specific Lectin from Aspergillus fumigatus

Tronchin¹,

Esnault²,

Sánchez³

et al. 2002

Infect Immun

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Adherence of the opportunistic fungus Aspergillus fumigatus to the extracellular matrix components is considered a crucial step in the establishment of the infection. Given the high carbohydrate content of these glycoproteins and the role of carbohydrate-protein interactions in numerous adherence processes, the presence of a lectin in A. fumigatus was investigated. Different fungal extracts obtained by sonication or grinding in liquid nitrogen from resting or swollen conidia, as well as from germ tubes and mycelium, were tested by hemagglutination assays using rabbit erythrocytes. A lectin activity was recovered in all the extracts tested. However, sonication of resting conidia resulted in the highest specific activity. Purification of the lectin was achieved by gel filtration followed by ion-exchange and hydrophobic-interaction chromatographies. Analysis of the purified lectin by sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed an apparent molecular mass of 32 kDa, which is similar to that of the alkaline protease already identified from different strains of A. fumigatus. However, as evidenced by the use of an alkaline protease-deficient mutant, the two activities were supported by distinct proteins. In addition, hemagglutination inhibition experiments using different saccharides and glycoproteins demonstrated the specificity of the lectin for sialic acid residues. Together these results suggest that this lectin may contribute to the attachment of conidia to the extracellular matrix components through the recognition of the numerous terminal sialic acid residues of their carbohydrate chains.

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Section: Discussionmentioning

confidence: 99%

Purification and Partial Characterization of a 32-Kilodalton Sialic Acid-Specific Lectin from Aspergillus fumigatus

Tronchin¹,

Esnault²,

Sánchez³

et al. 2002

Infect Immun

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“…Lectins of P. monodon(RATANAPO & CHULAVATNANOL, 1992;LUO et al, 2006), F. californiensis(VARGAS- ALBORES et al, 1993) Parapenaeus longirostris(FRAGKIADAKIS & STRATAKIS 1995) and M. rosenbergii(VÁZQUEZ et al, 1997) were able to agglutinate different species of bacteria, some of which are able to agglutinate pathogenic species of theVibrio genus. Quantitation of intracellular production of superoxide anions stimulated by the inductors used only demonstrated a significant difference (p<0.05) in the laminarin inductor in relation to the baseline in the control group, whereas in the group treated with probiotics Bacillus spp., all inductors were able to significantly increase (p<0.05) O 2 -production, except for the LPS inductor (Figure 3).…”

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confidence: 99%

Microbial biofloc as source of probiotic bacteria for the culture of Litopenaeus vannamei

et al. 2015

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“…Lectins are the most likely candidates for recognizing foreign material in invertebrates, due to their high specificity for carbohydrates [47]. They are involved in microorganism recognition by the binding of hemolymph agglutinins, and many reports have studied the specificity of crustacean lectins towards different bacteria.…”

Section: Discussionmentioning

confidence: 99%

Purification, characterization and induction of a C-type lectin in the freshwater planarian Dugesia japonica

et al. 2012

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Lectins are important components of the immune defense system of invertebrates. Given their important functions, numerous investigations have been carried out on the characterization and function of lectins in invertebrates. However, lectin studies with the freshwater planarian, an evolutionarily important animal, are rare. In this paper, we demonstrate agglutination of glutaraldehyde treated erythrocytes by a lectin with preference for rabbit erythrocytes. The result of hemagglutinating activity inhibition assays with several carbohydrates showed the most potent inhibitor was maltose. A natural lectin from the crude homogenates of freshwater planarian Dugesia japonica was purified by single step affinity chromatography using amylose-coupled agarose. The purified protein appeared as one band with a molecular mass of 350 kDa in PAGE, and as one band, approximately 56 kDa, in SDS-PAGE. The purified lectin showed dependence on calcium. The activity of the purified lectin was inhibited at temperatures greater than 50°C and showed a pH optimum between 5–8. The purified lectin also has binding activity to the Gram-negative bacteria E. coli, and the Gram-positive bacteria B. subtilis. Furthermore, the purified lectin obtained from injured and bacteria-induced planarians showed increased agglutinating activity against rabbit erythrocytes. These results suggest that the purified lectin may play an important role in the innate immunity of the freshwater planarian.

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Participation of a sialic acid-specific lectin from freshwater prawnMacrobrachium rosenbergii hemocytes in the recognition of non-self cells

Cited by 43 publications

References 25 publications

Purification and Partial Characterization of a 32-Kilodalton Sialic Acid-Specific Lectin from Aspergillus fumigatus

Purification and Partial Characterization of a 32-Kilodalton Sialic Acid-Specific Lectin from Aspergillus fumigatus

Microbial biofloc as source of probiotic bacteria for the culture of Litopenaeus vannamei

Purification, characterization and induction of a C-type lectin in the freshwater planarian Dugesia japonica

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