Participation of Bats (Chiroptera, Mammalia) and Their Ectoparasites in Circulation of Pathogens of Natural Focal Infections in the South of Russia

Zabashta, M. V.; Orlova, Maria V.; Пичурина, Н. Л.; Khametova, A. P.; Romanova, L. V.; Бородина, Т. Н.; Zabashta, A. V.

doi:10.1134/s0013873819040110

Cited by 12 publications

(10 citation statements)

References 19 publications

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“…Among all the seven positive samples for Anaplasma spp., only the amplicon obtained from O. hasei tick larva was sequenced, obtaining 97.6% similarity with A. phagocytophilum, which was previously detected in deer of the species Hydropotes inermis from the Republic of Korea. Previously, the occurrence of A. phagocytophilum was described in 22.6% (63/278) of fecal samples of Rhinolophus hipposideros in France [35], 1.7% (1/59) of blood samples of Myotis myotis [54] in Poland, 4.3% (6/138) of I. simplex ticks collected from bats from Hungary and Romania [36], and 14.28% (1/7) of Nyctalus noctula species from Russia [37]. Despite the high occurrence of A. phagocytophilum in bats from France, the remaining abovementioned studies showed low positivity for this agent among bats or associated ectoparasites, corroborating the results found in the present work for Anaplasma spp.…”

Section: Discussionmentioning

confidence: 99%

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Molecular Survey of Anaplasmataceae Agents and Coxiellaceae in Non-Hematophagous Bats and Associated Ectoparasites from Brazil

et al. 2021

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The Anaplasmataceae family (order Rickettsiales) encompasses obligately intracellular bacteria of the genera Anaplasma, Ehrlichia, and Neorickettsia. Together with Coxiella burnetii (Coxiellaceae family, order Legionellales), these bacteria represent important causative agents of diseases in humans and animals. The scarcity of studies that investigated the occurrence of these agents in bats and their associated ectoparasites, emphasizes the need to achieve a better understanding of the role of these animals in the maintenance of such bacteria. Herein, 418 samples (133 blood, 135 spleen, and 150 ectoparasites) are collected from 135 non-hematophagous bats belonging to 12 species in a periurban area of Campo Grande city, Mato Grosso do Sul state, midwestern Brazil. In the results, 1.65% (7/418), 12.04% (50/418), and 13.63% (57/418) of samples are positive in PCR assays for Anaplasma spp. (16S rRNA gene), Ehrlichia spp. (dsb gene), and Neorickettsia spp. (16S rRNA gene), respectively. Anaplasma spp. and Neorickettsia spp. are detected in one (5.26%) Ornithodoros hasei tick larva. Ehrlichia spp. is detected in 14% of bat flies (represented by Megistopoda aranea, Trichobius costalimai, and Strebla hertigi), 6% of tick larvae (O. hasei), 12% of Spinturnicidae mites (represented by Periglischrus sp., P. torrealbai, and P. acutisternus), and 38% of Macronyssidae mites (Steatonyssuss sp.). The obtained sequences are observed to be similar to Anaplasma phagocytophilum (97.42–97.6% identified), Ehrlichia minasensis (96.73–100% identified), Neorickettsia risticii (96.7–100% identified), and Neorickettsia findlayensis (95.07–100% identified) by BLASTn analyses, and closely related to Ehrlichia ruminantium by phylogenetic analyses based on the gltA gene. No bat samples (blood/spleen) are positive in the qPCR assay for C. burnetii based on the IS1111 gene. The present work shows, for the first time, the occurrence of Anaplasmataceae in bats and associated ectoparasites (ticks, mites, and bat flies) from Brazil.

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Section: Discussionmentioning

confidence: 99%

“…The DNA of A. phagocytophilum and Ehrlichia spp. was found in Pipistrellus pipistrellus and P. kuhlii bats, respectively, in Russia [37]. Ehrlichia spp.…”

Section: Introductionmentioning

confidence: 96%

Molecular Survey of Anaplasmataceae Agents and Coxiellaceae in Non-Hematophagous Bats and Associated Ectoparasites from Brazil

et al. 2021

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“…Borrelia, Rickettsia, Ehrlichia, Bartonella, Coxiella and Babesia species have previously been molecularly identified in C. vespertilionis from Europe [18,22,23,26,27,29]. It is often suggested that these putatively zoonotic microbes are vector-borne, i.e., tick-borne pathogens, presumably causing disease in bats and/or humans.…”

Section: Discussionmentioning

confidence: 99%

“…A total of 86% (29 of 34) of museum specimens of C. vespertilionis ticks collected between 1896 and 1994 in the United Kingdom contained DNA of Borrelia burgdorferi sensu lato as revealed by PCR [21]. B. burgdorferi s.l., the causative agent of the most common tick-borne human disease, Lyme borreliosis in the Holarctic Region, has also been detected in C. vespertilionis in southern Russia [22]. In France, C. vespertilionis ticks harbored a new genotype of the spotted fever group of rickettsiae called Rickettsia sp.…”

Section: Introductionmentioning

confidence: 99%

First Record of a Suspected Human-Pathogenic Borrelia Species in Populations of the Bat Tick Carios vespertilionis in Sweden

Jaenson

Wilhelmsson

2021

Microorganisms

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The bat tick Cariosvespertilionis has been reported from Sweden to occasionally feed on humans resulting in disease symptoms. The aim of this study was to investigate C. vespertilionis as a potential vector and reservoir of Borrelia species. In 2015 and 2018 in south-central Sweden, C. vespertilionis ticks were collected from a wooden bat box harboring Soprano pipistrelle bats, Pipistrellus pygmaeus. In addition, one C. vespertilionis tick found inside a house in southern Sweden in 2019 was collected. Ticks were screened for Borrelia spp. using a genus-specific quantitative PCR assay. The Borrelia species of the positive specimens were determined by conventional PCR followed by DNA sequencing and phylogenetic analyses. A total of 24% (22 of 92) of the analyzed C. vespertilionis ticks were Borrelia-positive. Phylogenetic analyses indicate that the bacteria belong to the relapsing fever group of borreliae; some of them appear to be identical with Borrelia sp. CPB1, a spirochete only found twice before—in the United Kingdom and in France. Our results also indicate a temporal and spatial distribution of this Borrelia species. Since C. vespertilionis occasionally bites humans, and since it exhibits a high prevalence of Borrelia bacteria, it is possible that it presents a risk of human disease. Further studies are needed to characterize Borrelia sp. CPB1 to determine if it is human-pathogenic and to determine if C. vespertilionis is a vector and/or reservoir of this agent.

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“…Rostov region, Astrakhan region (Zabashta et al, 2019;present article); Dagestan (present article) Algeria (Bendjeddou et al, 2013(Bendjeddou et al, , 2017; Tunisia (Beaucournu and Kock, 1996); Morocco (Quetglas et al, 2014);…”

Section: Ischnopsyllus Octactenus (Kolenati 1856)mentioning

confidence: 90%

Ectoparasites and Pathogens of Kuhl’s Pipistrelle Pipistrellus kuhlii (Kuhl, 1817) (Chiroptera: Vespertilionidae): Our Own and Published Data Review

Orlova

Smirnov

Вехник

et al. 2020

Russ J Biol Invasions

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Here we report the results of our own survey and literary published data on the ectoparasite fauna and pathogens of the alien bat species, the Kuhl’s pipistrelle Pipistrellus kuhlii (Kuhl, 1817) (Chiroptera: Vespertilionidae). This bat is a host of 36 species of parasitic mites, ticks and insects (including accidental findings) and 13 species of pathogens (protozoa, bacteria, viruses). The flea Ischnopsyllus variabilis is recorded on this host for the first time. We have found that outside of the host ancestral range, the core of the bat parasite fauna is significantly different due to the loss of host species-specific ectoparasites. Particularly, in Russia, only 6 species of parasitic arthropods have been recorded for Kuhl’s pipistrelle and all of them are host genus-specific. At the same time, the features of ecology and occasional finds of extrinsic parasites allow to suggest that P. kuhlii has wide contacts with animals which are the reservoirs of zoonotic infections, that in combination with the fact of isolation of several pathogens from this species (including two coronaviruses) points to a possible medical importance of Kuhl’s pipistrelle.

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Participation of Bats (Chiroptera, Mammalia) and Their Ectoparasites in Circulation of Pathogens of Natural Focal Infections in the South of Russia

Cited by 12 publications

References 19 publications

Molecular Survey of Anaplasmataceae Agents and Coxiellaceae in Non-Hematophagous Bats and Associated Ectoparasites from Brazil

Molecular Survey of Anaplasmataceae Agents and Coxiellaceae in Non-Hematophagous Bats and Associated Ectoparasites from Brazil

First Record of a Suspected Human-Pathogenic Borrelia Species in Populations of the Bat Tick Carios vespertilionis in Sweden

Ectoparasites and Pathogens of Kuhl’s Pipistrelle Pipistrellus kuhlii (Kuhl, 1817) (Chiroptera: Vespertilionidae): Our Own and Published Data Review

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