Participation of Cultured Mesenchymal Multipotent Stromal Cells in Regeneration of a Large Persisting Defect of Rabbit Radius Bone

Abstract

Abstract:The aim of this work was to characterize the impact of autologous cultured mesenchymal multipotent stromal cells (MMSCs) on the repair of large persisting defect in rabbit radius bone. MMSCs were obtained from femoral bone marrow aspirate, cultivated during 2-3 passages and marked by lentiviral vectors containing genes encoding green or cherry fluorescent proteins 4-6 days before implantation. The implantation of MMSCs resulted in new bone formation within 12 weeks. Cultured MMSC differentiated into bone, cartilage and other connective tissues in vivo. Mineral matrix used as a carrier of MMSС was completely resorbed in 10 weeks. Implanted MMSCs bearing marking gene maintained newly formed bone and cartilage tissues for at least one year. Thus, cultured MMSCs preserve the ability to differentiate and maintain bone tissue for long-term period in vivo, suggesting their high proliferative potential. Therefore, they could be used in tissue engineering in the healing of large bone defects.