Participation of Fos protein at the nucleus tractus solitarius in inhibitory modulation of baroreceptor reflex response in the rat

Shih, Cheng-Dean; Chan, Samuel H.H.; Chan, Julie Y.H.

doi:10.1016/0006-8993(96)00771-8

Cited by 38 publications

(31 citation statements)

References 28 publications

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“…The c-fos AS-ODN used in our experiments has been shown previously to successfully inhibit c-Fos expression in rat brain and spinal cord following topical application (Chiasson et al 1992;Dragunow et al 1993;Heilig et al 1993;Sommer et al 1993;Hooper et al 1994;Merchant 1994;M6ller et al 1994;Hunter et al 1995;Quercia and Chang 1996;Shih et al 1996). Similarly, using an intrahippocampal application protocol in the present study, we observed an efficient suppression of cFos IR in hippocampal and cortical regions ipsilateral to the injection site of c-fos AS-ODN compared with the contralateral, control ODN-injected hemisphere of the same rat after brightness discrimination training (Fig.…”

Section: Discussionmentioning

confidence: 78%

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Suppression of c-fos induction in rat brain impairs retention of a brightness discrimination reaction.

Grimm¹,

Schicknick²,

Riede³

et al. 1997

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IntroductionRecently, the induction of transcription factor-encoding immediate-early genes such as c-fos was observed in distinct brain regions of rats trained to acquire a footshock-motivated brightness discrimination in a Y-maze. The functional relevance of inducible transcription factors for learning and memory formation is, however, not clear. To address this question in the present study, we have used a synthetic antisense phosphorothioate oligodeoxynucleotide to suppress in vivo the expression of c-fos in rat brain.

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Section: Discussionmentioning

confidence: 78%

“…A significant suppression of c-Fos IR within a period of 3 hr following intracerebral administration of c-fos AS-ODN has been demonstrated recently (Shih et al 1996).…”

Section: Intrahippocampal Injectionsmentioning

confidence: 87%

Suppression of c-fos induction in rat brain impairs retention of a brightness discrimination reaction.

Grimm¹,

Schicknick²,

Riede³

et al. 1997

Learn. Mem.

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“…Three 15-mer phosphorothioated c-fos oligonucleotides (Quality System, Taipei, Taiwan) were used, [22][23][24] including a key antisense oligonucleotide (ASON1) that targets against a region of the c-fos mRNA that flanks the initiation codon (5Ј-129 to 143-3Ј) and a corresponding sense (SON) and a scrambled (ASON2) c-fos oligonucleotide. Each oligonucleotide was microinjected bilaterally, at a volume of 50 nL, into the NTS (nϭ25 to 30 animals per group) under pentobarbital anesthesia (50 mg/kg IP) 24 hours before the experimental sessions.…”

Section: Microinjection Of Test Agentsmentioning

confidence: 99%

“…[21][22][23][24] A rabbit polyclonal anti-Fos antiserum (sc-52, 1:4000; Santa Cruz) was used, and Fos-like immunoreactivity (Fos-LI) was visualized by a Vectastain ABC kit (Vector). As a routine, sections from both SHR and WKY that received the same treatment schedule were processed together.…”

Section: Immunohistochemical Stainingmentioning

confidence: 99%

“…The criterion for identification of Fos-LI was a distinctly stained nucleus, and the number of NTS neurons that displayed Fos-LI was quantified as reported previously. [21][22][23][24] Double immunohistochemical staining for Fos-LI and AT1R or AT2R was processed in some experiments as described. 29 In brief, sections were similarly processed for Fos-LI, using a polyclonal goat anti-Fos antiserum (sc-52G, 1:4000; Santa Cruz) and visualized by a Vectastain ABC peroxidase kit with nickel intensification.…”

Section: Immunohistochemical Stainingmentioning

confidence: 99%

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Augmented Upregulation by c- fos of Angiotensin Subtype 1 Receptor in Nucleus Tractus Solitarii of Spontaneously Hypertensive Rats

Chan

Ling-lin²,

Lee³

et al. 2002

Hypertension

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Abstract-Our laboratory demonstrated previously that spontaneously hypertensive rats (SHR) exhibited an elevated basal Fos expression in the nucleus tractus solitarii (NTS), the terminal site for primary baroreceptor afferents, and that Fos protein is required for the re-expression of angiotensin subtype 1 receptor (AT1R) mRNA in the NTS after baroreceptor activation. The present study evaluated the hypothesis that this re-expression of AT1R is augmented in SHR and is promoted by the heightened Fos expression. Reverse transcription-polymerase chain reaction analysis revealed that baroreceptor activation via sustained increase in systemic arterial pressure resulted in a discernible reduction in the expression of AT1R mRNA at the dorsomedial medulla of SHR and normotensive Wistar-Kyoto rats. However, SHR manifested an appreciably larger magnitude of decline, followed by a faster time course of re-expression in AT1R mRNA. Parallel findings were obtained from the pressor response induced by microinjection unilaterally of angiotensin II (40 pmol) into the NTS. Whereas the re-expression of AT1R at both transcriptional and functional expression levels after baroreceptor activation was discernibly blunted by prior bilateral application into the NTS of an antisense c-fos oligonucleotide (50 pmol), the suppression in SHR was again significantly more intense. Control pretreatment with the corresponding sense or scrambled c-fos oligonucleotide was ineffective. We conclude that the heightened Fos expression in SHR is causatively related to the augmented re-expression of AT1R in the NTS at both transcriptional and functional levels.

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Preclinical safety assessments of nano‐sized constructs on cardiovascular system toxicity: A case for telemetry

Cheah

Kiew

Lee

et al. 2017

J of Applied Toxicology

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While nano-sized construct (NSC) use in medicine has grown significantly in recent years, reported unwanted side effects have raised safety concerns. However, the toxicity of NSCs to the cardiovascular system (CVS) and the relative merits of the associated evaluation methods have not been thoroughly studied. This review discusses the toxicological profiles of selected NSCs and provides an overview of the assessment methods, including in silico, in vitro, ex vivo and in vivo models and how they are related to CVS toxicity. We conclude the review by outlining the merits of telemetry coupled with spectral analysis, baroreceptor reflex sensitivity analysis and echocardiography as an appropriate integrated strategy for the assessment of the acute and chronic impact of NSCs on the CVS.

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Participation of Fos protein at the nucleus tractus solitarius in inhibitory modulation of baroreceptor reflex response in the rat

Cited by 38 publications

References 28 publications

Suppression of c-fos induction in rat brain impairs retention of a brightness discrimination reaction.

Suppression of c-fos induction in rat brain impairs retention of a brightness discrimination reaction.

Augmented Upregulation by c- fos of Angiotensin Subtype 1 Receptor in Nucleus Tractus Solitarii of Spontaneously Hypertensive Rats

Preclinical safety assessments of nano‐sized constructs on cardiovascular system toxicity: A case for telemetry

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