Participation of GABA-ergic system in epileptogenic activity induced by teicoplanin in mice

Takechi, Kenshi; Fujiwara, Akinori; Watanabe, Yusuke; Kamei, Chiaki

doi:10.1016/j.eplepsyres.2009.01.006

Cited by 6 publications

(5 citation statements)

References 19 publications

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Section: Discussionsupporting

confidence: 53%

“…In agreement with these results, the intracerebroventricular administration of 5 μg (31,055 pmol) TPMPA to mice also failed to induce seizures (Takechi et al 2009). Seizures developed when 10 μg of TPMPA had been injected (Takechi et al 2009). However, under such high dosage conditions, it may be possible that in addition to blocking GABA A ρ receptors TPMPA had blocked conventional GABA A receptors, in this way eliciting convulsive behavior.…”

Section: Discussionsupporting

confidence: 53%

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GABAA ρ receptor mechanisms in the rat amygdala and its role in the modulation of fear and anxiety

et al. 2010

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Section: Discussionsupporting

confidence: 53%

Section: Discussionsupporting

confidence: 53%

GABAA ρ receptor mechanisms in the rat amygdala and its role in the modulation of fear and anxiety

et al. 2010

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“…Normal saline was applied for dilution of Dic and morphine as positive controls. The selected concentrations of drugs were based on the previous literature and the outcomes of our initial experiments ( 10 16 17 18 19 20 21 ). Furthermore, because normal saline or DMSO administrations have shown the same results without any acute or chronic toxicity, we decided to use DMSO as a control or vehicle in all experiments.…”

Section: Methodsmentioning

confidence: 99%

Effects of intrathecal and intracerebroventricular microinjection of kaempferol on pain

Jabbari

Bananej

Zarei

et al. 2021

Research in Pharmaceutical Sciences

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Background and purpose: Kaempferol (KM), a flavonoid, has an anti-inflammatory and anticancer effect and prevents many metabolic diseases. Nonetheless, very few studies have been done on the antinociceptive effects of KM. This research aimed at assessing the involvement of opioids, gamma-aminobutyric acid (GABA) receptors, and inflammatory mediators in the antinociceptive effects of KM in male Wistar rats. Experimental approach: The intracerebroventricular and/or intrathecal administration of the compounds was done for examining their central impacts on the thermal and chemical pain by the tail-flick and formalin paw tests. For assessing the role of opioid and GABA receptors in the possible antinociceptive effects of KM, several antagonists were used. Also, a rotarod test was carried out for assessing motor performance. Findings/Results: The intracerebroventricular and/or intrathecal microinjections of KM (40 μg/rat) had partially antinociceptive effects in the tail-flick test in rats ( P < 0.05). In the formalin paw model, the intrathecal microinjection of KM had antinociceptive effects in phase 1 (20 and 40 μg/rat; P < 0.05 and P < 0.01, respectively) and phase 2 (20 and 40 μg/rat; P < 0.01 and P < 0.001, respectively). Using naloxonazine and/or bicuculline approved the involvement of opioid and GABA receptors in the central antinociceptive effects of KM, respectively. Moreover, KM reduced the expression levels of caspase 6, interleukin-1β, tumor necrosis factor-α, and interleukin-6. The antinociceptive effects of KM were not linked to variations in the locomotor activity. Conclusion and implications: It can be concluded that KM has remarkable antinociceptive effects at a spinal level, which is associated with the presence of the inflammatory state. These impacts were undetectable following injections in the lateral ventricle. The possible mechanisms of KM antinociception are possibly linked to various modulatory pathways, including opioid and GABA receptors.

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“…The solutions were prepared immediately prior to testing. The selected concentrations of KM and all drugs were based on previous studies and outcomes of the initial experiments of the authors of the present study [9,[15][16][17][18][19][20]. Furthermore, since NS or DMSO administration has shown the same results without any acute or chronic toxicity, it was decided to use DMSO as a control or vehicle (Veh) group in all the experiments.…”

Section: Drugs and Chemical Compoundsmentioning

confidence: 99%

Possible Involvement of Serotonergic Mechanism(s) in the Antinociceptive Effects of kaempferol

Jabbari

Bananej

Zarei

et al. 2020

ajnpp

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Background and Objectives: A flavonoid kaempferol (KM) exerts an anti-inflammatory effect and is reportedly capable of preventing metabolic diseases. Nonetheless, a limited number of studies have been carried out on the antinociceptive effects of kaempferol. Objectives: The present study aimed to investigate the involvement of serotonin receptors in the antinociceptive-like activity of KM in male Wistar rats using the tail-flick test. Materials and Methods: The compounds (i.e., KM, morphine, and diclofenac) were intracerebroventricularly administered to rats for the examination of central effects on the thermal pain using the tail-flick test. For the evaluation of the involvement of serotonin receptors in the possible antinociceptive effects of kaempferol, several antagonists (i.e., tropisetron, ketanserin, GR113808, WAY 100635, and penbutolol) were used. Additionally, locomotor activity and motor responses were investigated by the rotarod test after KM treatment. Results: The intracerebroventricular microinjections of KM showed antinociceptive effects using the tail-flick test. The pretreatment with tropisetron as a 5-HT3 receptor antagonist at 1 and 10 mg completely reversed the KM-related antinociception. Furthermore, ketanserin (5-HT2A receptor antagonist) and GR113808 (5-HT4 receptor antagonist) both at 10 mg reduced KM-related antinociception; however, 5-HT1A receptor antagonist WAY 100635 and 5-HT1B antagonist penbutolol did not decrease KM-related antinociception. All KM doses were not observed with a significant effect on locomotor activity or motor reactions. Conclusion: The results of the current study suggested that serotonergic receptors (i.e., 5-HT2A, 5-HT3, and 5-HT4) are effective in the KM antinociceptive activity in male rats.

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Participation of GABA-ergic system in epileptogenic activity induced by teicoplanin in mice

Cited by 6 publications

References 19 publications

GABAA ρ receptor mechanisms in the rat amygdala and its role in the modulation of fear and anxiety

GABAA ρ receptor mechanisms in the rat amygdala and its role in the modulation of fear and anxiety

Effects of intrathecal and intracerebroventricular microinjection of kaempferol on pain

Possible Involvement of Serotonergic Mechanism(s) in the Antinociceptive Effects of kaempferol

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