Participation of lipid peroxidation in the loss of hepatic drug-metabolizing activities in experimental fascioliasis in the rat

Facino, Roberto Maffei; Carini, Marina; Genchi, C.; Tofanetti, O.; Casciarri, I.

doi:10.1016/1043-6618(89)90196-5

Cited by 24 publications

(12 citation statements)

References 15 publications

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“…(Galtier et al 1987). The development of lipid peroxidation has previously been described in livers of rats infected by Fasciola (Fancino et al 1989;Galtier et al 1994). Such an oxidative cell injury has been suggested to occur in the course of fasciolosis as the consequence of tissue destruction produced by toxic secretions of thē ukes (Galtier et al 1987).…”

Section: Discussionmentioning

confidence: 87%

Oxidative stress and changes in liver antioxidant enzymes induced by experimental dicroceliosis in hamsters

Sánchez-Campos¹,

Tuñón²,

González³

et al. 1999

Parasitology Research

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The purpose of our study was to assess the effects of experimental dicroceliosis on the antioxidant defense capability of the liver in hamsters. Studies were carried out at 80 and 120 days after infection with an oral dose of 40 metacercariae of Dicrocoelium dendriticum. The parasitic pathology was ascertained by the presence of fluke eggs in feces, increased serum ALT and AST activities, and histological findings. The concentration of thiobarbituric acid-reactive substances (TBARS) and the ratio of oxidized to reduced glutathione (GSSG/GSH), measured as markers of oxidative stress, were significantly increased [TBARS: +40% and +84% at 80 and 120 days postinfection (p.i.), respectively; GSSG/GSH: +200% and +117%]. Dicroceliosis increased Se-dependent glutathione peroxidase (GPx) activity in both cytosol (+24% and +46%) and mitochondria (+73% and +41%). Superoxide dismutase activity was significantly reduced in cytosol (-19% and -38%) and mitochondria (-20% and -39%). No significant change was found in the activity of Se-independent GPx or catalase. The ratio of glutathione peroxidase/glutathione reductase at 80 and 120 days p.i. was increased by 25% and 63%, respectively. Gamma-glutamyl cysteinyl synthetase activity was increased by 27% and 20%, respectively. Our data indicate that although dicroceliosis courses with activation of antioxidant enzymes and glutathione synthesis, inefficient scavenging of reactive oxygen species takes place, resulting in oxidative liver damage.

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Section: Discussionmentioning

confidence: 87%

Oxidative stress and changes in liver antioxidant enzymes induced by experimental dicroceliosis in hamsters

Sánchez-Campos¹,

Tuñón²,

González³

et al. 1999

Parasitology Research

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“…Eventually, inflammation during the chronic phase of fasciolosis spreads to the bile ducts (Dawes 1963;Thorpe 1965;Kolodziejczyk 1994). This destructive process is invariably associated with chemical alterations in the cell, such as enhancement of membrane lipid peroxidation and marked suppression of the microsomal drug-metabolizing mono-oxygenase system (Maffei Facino et al 1989;. In murine liver infected with the fluke, augmented activities of some enzymes participating in the Krebs cycle and the respiratory chain herald changes in the rate of oxidative reactions (Kolodziejczyk 1996).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant potential of rat liver in experimental infection with Fasciola hepatica

2005

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The aim of this paper is to assess the antioxidant properties of rat liver in the course of acute and chronic fasciolosis. Wistar rats were infected per os with 30 metacercariae of Fasciola hepatica. Liver activities of antioxidant enzymes and concentrations of non-enzymatic antioxidants were determined at 4, 7, and 10 weeks post-infection. Activities of superoxide dismutase (Cu,Zn-SOD), glutathione peroxidase (GSH-Px), and glutathione reductase (GSSG-R) were decreased, catalase (CAT) activity was increased and non-enzymatic antioxidant concentrations (reduced glutathione, vitamins C, E and A) were reduced simultaneously with enhancement of lipid peroxidation processes as evidenced by increased levels of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE). Changes in the antioxidant abilities of the liver and in the phospholipid structure of the cell membrane were accompanied by rising activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) as markers of liver damage.

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“…6 Some oxidative parameters have been studied in few human and animal patients with F. hepatica. 6,7,8 In this study, we evaluated the oxidative status in patients with F. hepatica and in healthy controls via measurement of serum oxidative stress markers, including total antioxidant capacity (TAC), total oxidative status (TOS), oxidative stress index (OSI), and catalase.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of oxidative status in patients with <i>Fasciola hepatica</i> infection

Karsen

Sünnetçioğlu²,

Ceylan³

et al. 2011

Af Hlth Sci

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AbsractBackground: F. hepatica infection is rare and mostly subclinical. Migration of juvenile forms of F. hepatica into the host's liver is accompanied by an inflammatory reaction followed by fibrosis and cirrhosis. Objective: The aim of this study was to evaluate oxidative status by using a novel automated method in patients with Fasciola hepatica. Methods: Twenty two patients with a diagnosis of F. hepatica and 26 healthy volunteers were enrolled in the study. Their Total antioxidant capacity status (TAC), total oxidant status (TOS) and catalase were measured in them and oxidative stress index (OSI) was calculated. These measurements were also taken for the control group and the values were compared. Results: Plasma levels of total TOS and OSI were significantly increased in patients as compared with healthy controls (P<0.001, P<0.001, P=0.001, P=0.008) respectively. In contrast, TAC level was significantly lower in patients as compared with controls (p<0.05). There was no significant difference between the catalase results of the two groups (p>0.05). Conclusion: Total oxidative status and OSI were increased and total antioxidative status capacity was decreased in patients with F. hepatica infeciton. A high oxidative stress occurs during F. hepatica infection, which may cause severe damage in both the liver.

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Participation of lipid peroxidation in the loss of hepatic drug-metabolizing activities in experimental fascioliasis in the rat

Cited by 24 publications

References 15 publications

Oxidative stress and changes in liver antioxidant enzymes induced by experimental dicroceliosis in hamsters

Oxidative stress and changes in liver antioxidant enzymes induced by experimental dicroceliosis in hamsters

Antioxidant potential of rat liver in experimental infection with Fasciola hepatica

Evaluation of oxidative status in patients with <i>Fasciola hepatica</i> infection

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