Participation of the<i>Candida albicans</i>surface antigen in adhesion, the first phase of biofilm development

Bujdáková, Helena; Paulovičová, Ema; Paulovičová, Lucia; Šimová, Zuzana

doi:10.1111/j.1574-695x.2010.00713.x

Cited by 18 publications

(15 citation statements)

References 40 publications

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“…Taking into consideration the results summarizing the degree of attached cells and kinetic pattern of adherence to polystyrene, the first 30 min of interaction seems to be equally critical for the cell attachment of tested strains of both spp. The observation that the first 30 min are critical for adhesion confirmed results previously described by Bujdakova et al [32] in biofilm model with the C. albicans standard strain SC 5314 and other clinical isolate than tested in this study.…”

Section: Discussionsupporting

confidence: 79%

“…However, the degree of colonization of any surface is not only dependent on the adhesive properties of individual strains, but the duration and kinetics of adhesion can also be important. The context of the interactions between Candida cells and their adhesion to various surfaces has recently been investigated [32][33][34][35]. The results of our study do not indicate a significant difference in adhesion to a polystyrene surface between the tested clinical and nonclinical strains, with the exception of C. dubliniensis 539.…”

Section: Discussionsupporting

confidence: 58%

“…of tested strains with mainly the yeast form observed in C. dubliniensis, in contrast to C. albicans, where the mycelial form was more prevalent. Adhesion to unrelated substrates has already been noted as an important step not only in biofilm formation [30][31][32], but can also be essential for colonization [3]. However, the degree of colonization of any surface is not only dependent on the adhesive properties of individual strains, but the duration and kinetics of adhesion can also be important.…”

Section: Discussionmentioning

confidence: 99%

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Biofilm formation and adhesive/invasive properties of Candida dubliniensis in comparison with Candida albicans

et al. 2011

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Candida dubliniensis and Candida albicans are closely related spp. exhibiting differences in their virulence potency. This study compared clinical isolates of C. dubliniensis with C. albicans from HIV patients with oropharyngeal candidiasis (OPC) and standard strains in power to form biofilm and their adhesive and invasive properties. Members of both spp. were able to form strong biofilms. However, SEM microscopy confirmed that C. albicans undergoes the more effective yeast-to-hyphae transition than C. dubliniensis with prevalent yeast form and limited ability to form filaments. Kinetic patterns indicated that while the first 30 min are critical for sufficient attachment to a polystyrene surface, adhesion to human carcinoma cell lines (Caco-2 and TR 146) needs additional time with maximal saturation observed at 240 min for both spp. The invasion process was tested on 3D RHE (reconstituted human epithelium) with Caco-2 or TR 146 on the collagen surface. C. albicans rapidly produced hyphae that penetrated the tissue layer, demonstrating substantive invasion within 21 h. In contrast, C. dubliniensis attached to the tissue surface and proliferated, suggesting the formation of a biofilm-like structure. After 21 h, C. dubliniensis was able to penetrate the RHE layer and invade unusually, with a cluster of the yeast cells

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Section: Discussionsupporting

confidence: 79%

Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

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Biofilm formation and adhesive/invasive properties of Candida dubliniensis in comparison with Candida albicans

et al. 2011

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“…A polyclonal anti- C. albicans antibody produced in chicken egg yolk prevented the adherence and biofilm formation by multiple Candida species (94). Antibody blocking of the C. albicans complement receptor 3-related protein significantly reduced adhesion and subsequent biofilm formation (95). Martinez and colleagues studied the effect of combinations of conventional antifungals and specific monoclonal antibodies against C. neoformans biofilms; however, the antibodies displayed a mostly antagonistic effect in combination with the antifungal drugs (96, 97).…”

Section: Development Of Novel Strategies and Therapeutics To Combat Amentioning

confidence: 99%

From Biology to Drug Development: New Approaches to Combat the Threat of Fungal Biofilms

et al. 2015

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Fungal infections constitute a major threat to an escalating number of critically ill patients. Fungi are eukaryotic organisms and, as such, there is a limited armamentarium of antifungal drugs, leading to high mortality rates. Moreover, fungal infections are often associated with the formation of biofilms, which contribute to virulence and further complicate treatment due to the high level of antifungal drug resistance displayed by sessile cells within these microbial communities. Thus, the treatment of fungal infections associated with a biofilm aetiology represents a formidable and unmet clinical challenge. The increasing importance and awareness of fungal biofilms is reflected by the fact that this is now an area of very active research. Studies in the last decade have provided important insights into fungal biofilm biology, physiology and pathology, as well as into the molecular basis of biofilm resistance. Here we discuss how this accumulated knowledge may inform the development of new anti-biofilm strategies and therapeutics that are urgently needed.

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“…However, their applicability in treating biofilm-related infections is still in a preliminary state. In that framework, recent data showed that pretreatment of C. albicans cells with antibodies targeting the complement receptor 3-related protein led to reduced adhesion and biofilm formation in vitro [169]. In another study, anti- C. albicans antibodies from chicken egg yolk were employed as antiadherent molecules [170].…”

Section: Antibiofilm Strategies: Research and Developmentmentioning

confidence: 99%

CandidaBiofilms and the Host: Models and New Concepts for Eradication

Tournu

Dijck

2012

International Journal of Microbiology

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Biofilms define mono- or multispecies communities embedded in a self-produced protective matrix, which is strongly attached to surfaces. They often are considered a general threat not only in industry but also in medicine. They constitute a permanent source of contamination, and they can disturb the proper usage of the material onto which they develop. This paper relates to some of the most recent approaches that have been elaborated to eradicate Candida biofilms, based on the vast effort put in ever-improving models of biofilm formation in vitro and in vivo, including novel flow systems, high-throughput techniques and mucosal models. Mixed biofilms, sustaining antagonist or beneficial cooperation between species, and their interplay with the host immune system are also prevalent topics. Alternative strategies against biofilms include the lock therapy and immunotherapy approaches, and material coating and improvements. The host-biofilm interactions are also discussed, together with their potential applications in Candida biofilm elimination.

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Participation of theCandida albicanssurface antigen in adhesion, the first phase of biofilm development

Cited by 18 publications

References 40 publications

Biofilm formation and adhesive/invasive properties of Candida dubliniensis in comparison with Candida albicans

Biofilm formation and adhesive/invasive properties of Candida dubliniensis in comparison with Candida albicans

From Biology to Drug Development: New Approaches to Combat the Threat of Fungal Biofilms

CandidaBiofilms and the Host: Models and New Concepts for Eradication

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