The Mobile Enterprise is a new form of enterprise in the contemporary mobile era. Although several well-known enterprise architecture frameworks are used by enterprises, it is apparent that there is no industry standard available to enable an enterprise to transform its business processes to incorporate mobile technologies to advantage. This article presents a conceptual Mobile Enterprise Architecture Framework and supporting methodology and process model which can aid enterprise decision makers to evaluate the business values, and analyze the risks and other critical business and technical factors for enterprise mobile initiatives and mobile transitions. The framework covers both the enterprise and mobile enterprise architecture domains that represent the Enterprise, Business, and Mobile Adoption levels. The goal at the Enterprise Level is to obtain a mobile enterprise and the technologies adopted at the Mobile Adoption Level are the different mobile technologies to be incorporated. Each level contains some important components impacting the mobile enterprise transformation. The methodology and process model cover the Strategy, Analysis, Design, Implementation, and Maintenance stages for each mobile initiative, and were validated in a research project against some Ontario Government mobile initiatives.