Particle designs for the stabilization and controlled-delivery of protein drugs by biopolymers: A case study on insulin

Lim, Hui Peng; Tey, Beng Ti; Chan, Eng Seng

doi:10.1016/j.jconrel.2014.04.042

Cited by 55 publications

(30 citation statements)

References 90 publications

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“…[34] In the absence of a macromolecular carrier, protein drugs are susceptible to low bioavailability and degradation by proteases in the bloodstream. [35] Consequently, a number of polymer-based hydrogels have been proposed for the effective encapsulation and release of protein molecules upon interaction with a targeted stimulus. [36] Utilising a hydrogel that incorporates a biopolymer as the gelator, in the absence of oil-derived and non-biodegradable polymers, has clear environmental benefits, particularly for use in an application that seeks to be environmentally beneficial, and for employment in vivo.…”

Section: Introductionmentioning

confidence: 99%

Chitosan hydrogels for targeted dye and protein adsorption

Boardman

Lad

Green

et al. 2017

J of Applied Polymer Sci

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There is an urgent requirement to devise and develop highly-effective materials for the purification of industrial wastewater. Coloured effluents are particularly problematic due to their toxicity in plants, aquatic organisms and animals, dictating that polymeric adsorbents are highly sought for their capture.Chitosan hydrogels are a cost-effective substrate for the adsorption of dye molecules as they are contain a biopolymeric gelator that can form hydrogels with a polymer content as low as 3% by mass. Such materials are also highly suited for use within a biomedical context as carrier vehicles for the encapsulation and delivery of protein macromolecules, due to their hydrophilic nature. We disclose the capability chitosan-based hydrogels to non-covalently adsorb both reactive and disperse dye molecules, and a model anionic protein from aqueous solution. The materials generated offer both a platform for dye removal from industrial wastewater, and for the encapsulation and pH-mediated release of protein macromolecules.

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Section: Introductionmentioning

confidence: 99%

Chitosan hydrogels for targeted dye and protein adsorption

Boardman

Lad

Green

et al. 2017

J of Applied Polymer Sci

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“…Insulin is commonly administered by daily subcutaneous (sc) injections, leading to low patient compliance and unsatisfactory metabolic regulation. 2 Oral insulin administration is more convenient, less invasive, and mimics physiological uptake via the hepatic route; 3 therefore, various oral insulindelivery systems such as liposomes, 4 emulsions, 5 microspheres, 6 and polymer-based nanoparticles (NPs) 7,8 have been developed. However, the bioavailability of orally administered insulin preparations is not satisfactory because of their inherent instabilities in the gastrointestinal tract and inefficient absorption due to low permeability across biological membranes.…”

Section: Introductionmentioning

confidence: 99%

Self-assembled lecithin/chitosan nanoparticles for oral insulin delivery: preparation and functional evaluation

Liu¹,

Zhou²,

Xia³

et al. 2016

IJN

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Purpose Here, we investigated the formation and functional properties of self-assembled lecithin/chitosan nanoparticles (L/C NPs) loaded with insulin following insulin–phospholipid complex preparation, with the aim of developing a method for oral insulin delivery. Methods Using a modified solvent-injection method, insulin-loaded L/C NPs were obtained by combining insulin–phospholipid complexes with L/C NPs. The nanoparticle size distribution was determined by dynamic light scattering, and morphologies were analyzed by cryogenic transmission electron microscopy. Fourier transform infrared spectroscopy analysis was used to disclose the molecular mechanism of prepared insulin-loaded L/C NPs. Fast ultrafiltration and a reversed-phase high-performance liquid chromatography assay were used to separate free insulin from insulin entrapped in the L/C NPs, as well as to measure the insulin-entrapment and drug-loading efficiencies. The in vitro release profile was obtained, and in vivo hypoglycemic effects were evaluated in streptozotocin-induced diabetic rats. Results Our results indicated that insulin-containing L/C NPs had a mean size of 180 nm, an insulin-entrapment efficiency of 94%, and an insulin-loading efficiency of 4.5%. Cryogenic transmission electron microscopy observations of insulin-loaded L/C NPs revealed multilamellar structures with a hollow core, encircled by several bilayers. In vitro analysis revealed that insulin release from L/C NPs depended on the L/C ratio. Insulin-loaded L/C NPs orally administered to streptozotocin-induced diabetic rats exerted a significant hypoglycemic effect. The relative pharmacological bioavailability following oral administration of L/C NPs was 6.01%. Conclusion With the aid of phospholipid-complexation techniques, some hydrophilic peptides, such as insulin, can be successfully entrapped into L/C NPs, which could improve oral bioavailability, time-dependent release, and therapeutic activity.

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“…In the review of George et al the application and limitation of chitosan and alginate on protein delivery by intestinal route was discussed (George and Abraham 2006). Except for as matrix material, chitosan was also widely used for particle coating (Lim et al 2014). Salmon calcitonin loaded lipid nanoparticles was coated by chitosan.…”

Section: Chitosan For Protein Deliverymentioning

confidence: 99%

Drug Delivery Applications of Chitosan and its Derivatives

Zhang

Mao

2015

Excipient Applications in Formulation Design and Drug Delivery

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The development of drug delivery systems always goes hand in hand with the advancement of material science. The novel synthetic or natural functional materials provide opportunities to design optimal drug delivery systems. Among these, chitosan and its derivatives exhibit excellent application future because of its prominent characteristics. In this chapter, research progress of chitosan and its derivatives for drug delivery, from structure to properties, from properties to its application were overviewed. The physicochemical properties, biological properties, biodegradability, safety evaluation and structure modification of chitosan were introduced. Furthermore, applications of chitosan and its derivatives in drug delivery including as the carrier of nanoparticles and microparticles, as coating material, as matrix of hydrogels, films and sustained release tablets, as stabilizers, as absorption enhancers, were discussed. Finally, the applications of chitosan and its derivatives in different administration routes, and their advantages for gene, protein and vaccine delivery, were reviewed. The numerous successful studies on exploitation and application of chitosan and its derivatives showed promising application future of these materials in drug delivery.

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Particle designs for the stabilization and controlled-delivery of protein drugs by biopolymers: A case study on insulin

Cited by 55 publications

References 90 publications

Chitosan hydrogels for targeted dye and protein adsorption

Chitosan hydrogels for targeted dye and protein adsorption

Self-assembled lecithin/chitosan nanoparticles for oral insulin delivery: preparation and functional evaluation

Drug Delivery Applications of Chitosan and its Derivatives

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