Particle-labelled immunoassays: A review

Gribnau, T.C.J.; Leuvering, Jan H.W.; Hell, H. van

doi:10.1016/s0378-4347(00)80835-x

Cited by 42 publications

(16 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This new immunoassay method showed promise since the technique was homogeneous, i.e., did not need a separation of the free antigen from bound complexes [3]. Unfortunately, the LSPR effect proved very sensitive to non-specific interactions and was barely applicable to crude serum or urine samples [79]. However when a suitable separation of the possible interfering components in the sample could be effected, the technique proved useful as shown later by its application to the determination of the affinity of monoclonal antibodies [80].…”

Section: Surface Plasmon Resonance With Colloidal Gold In Solutionmentioning

confidence: 99%

Surface plasmon resonance: principles, methods and applications in biomedical sciences

Englebienne

Hoonacker

Verhas

2003

Journal of Spectroscopy

297

140

View full text Add to dashboard Cite

Surface plasmon resonance (SPR) is a phenomenon occuring at metal surfaces (typically gold and silver) when an incident light beam strikes the surface at a particular angle. Depending on the thickness of a molecular layer at the metal surface, the SPR phenomenon results in a graded reduction in intensity of the reflected light. Biomedical applications take advantage of the exquisite sensitivity of SPR to the refractive index of the medium next to the metal surface, which makes it possible to measure accurately the adsorption of molecules on the metal surface and their eventual interactions with specific ligands. The last ten years have seen a tremendous development of SPR use in biomedical applications. The technique is applied not only to the measurement in real-time of the kinetics of ligand–receptor interactions and to the screening of lead compounds in the pharmaceutical industry, but also to the measurement of DNA hybridization, enzyme–substrate interactions, in polyclonal antibody characterization, epitope mapping, protein conformation studies and label-free immunoassays. Conventional SPR is applied in specialized biosensing instruments. These instruments use expensive sensor chips of limited reuse capacity and require complex chemistry for ligand or protein immobilization. Our laboratory has successfully applied SPR with colloidal gold particles in buffered solution. This application offers many advantages over conventional SPR. The support is cheap, easily synthesized, and can be coated with various proteins or protein–ligand complexes by charge adsorption. With colloidal gold, the SPR phenomenon can be monitored in any UV-vis spectrophotometer. For high‒throughput applications, we have adapted the technology in an automated clinical chemistry analyzer. This simple technology finds application in label-free quantitative immunoassay techniques for proteins and small analytes, in conformational studies with proteins as well as in the real-time association-dissociation measurements of receptor–ligand interactions, for high-throughput screening and lead optimization.

show abstract

Section: Surface Plasmon Resonance With Colloidal Gold In Solutionmentioning

confidence: 99%

Surface plasmon resonance: principles, methods and applications in biomedical sciences

Englebienne

Hoonacker

Verhas

2003

Journal of Spectroscopy

297

140

View full text Add to dashboard Cite

show abstract

“…Analytical applications of paper in science date to the early 17th century with the use of cellulose papers for chromatographic purposes [6] and pH sensing [7,8]. A paper-based dipstick for quantifying glucose in urine was first introduced to the scientific community in the 1950s, followed by commercialization of the product for a diabetes test one decade later [9].…”

Section: Introductionmentioning

confidence: 99%

Paper and Fiber-Based Bio-Diagnostic Platforms: Current Challenges and Future Needs

2017

View full text Add to dashboard Cite

Abstract:In this perspective article, some of the latest paper and fiber-based bio-analytical platforms are summarized, along with their fabrication strategies, the processing behind the product development, and the embedded systems in which paper or fiber materials were integrated. The article also reviews bio-recognition applications of paper/fiber-based devices, the detected analytes of interest, applied detection techniques, the related evaluation parameters, the type and duration of the assays, as well as the advantages and disadvantages of each technique. Moreover, some of the existing challenges of utilizing paper and/or fiber materials are discussed. These include control over the physical characteristics (porosity, permeability, wettability) and the chemical properties (surface functionality) of paper/fiber materials are discussed. Other aspects of the review focus on shelf life, the multi-functionality of the platforms, readout strategies, and other challenges that have to be addressed in order to obtain reliable detection outcomes.

show abstract

“…The development of this type of novel family of fiber-based materials requires understanding of the possible mechanisms for detection of a given liquid when it invades the pores of the fibrous material. In many applications, when dealing with a minute amount of liquid, fiber-based colorimetric biosensors seem to be the most popular [2][3][4][5]. However, in other situations, when the probe and sensor are hidden in an internal part of the material, making the color change impossible to detect, another mechanism of liquid detection is needed.…”

Section: Introductionmentioning

confidence: 99%

Elastocapillarity: Stress transfer through fibrous probes in wicking experiments

Monaenkova

Kornev

2010

Journal of Colloid and Interface Science

View full text Add to dashboard Cite

Particle-labelled immunoassays: A review

Cited by 42 publications

References 24 publications

Surface plasmon resonance: principles, methods and applications in biomedical sciences

Surface plasmon resonance: principles, methods and applications in biomedical sciences

Paper and Fiber-Based Bio-Diagnostic Platforms: Current Challenges and Future Needs

Elastocapillarity: Stress transfer through fibrous probes in wicking experiments

Contact Info

Product

Resources

About