Particles 3D tracking with large axial depth by using the 2π-DH-PSF

Li, Hangfeng; Wang, Famin; Wei, Tao; Miao, Xin; Cheng, Yuanda; Pang, Xinpei; Jiang, Keming; Huang, Wei; Zhang, Yunhai

doi:10.1364/ol.434981

Cited by 10 publications

(3 citation statements)

References 17 publications

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“…This framework aims to extract maximum physical information about the position of a single nanoscale object from an image. The research group of Zhang Yunhai at the Suzhou Institute of Biomedical Engineering Technology, Chinese Academy of Sciences, has proposed a 2π-DH-PSF using the Fresnel zone approach that can rotate 2π radians, showcasing its superior performance in the 3D localization imaging of nanoparticles [12].…”

Section: Introductionmentioning

confidence: 99%

Analytical Model of Point Spread Function under Defocused Degradation in Diffraction-Limited Systems: Confluent Hypergeometric Function

Song,

Chen,

Tang

et al. 2024

Photonics

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In recent years, optical systems near the diffraction limit have been widely used in high-end applications. Evidently, an analytical solution of the point spread function (PSF) will help to enhance both understanding and dealing with the imaging process. This paper analyzes the Fresnel diffraction of diffraction-limited optical systems in defocused conditions. For this work, an analytical solution of the defocused PSF was obtained using the series expansion of the confluent hypergeometric functions. The analytical expression of the defocused optical transfer function is also presented herein for comparison with the PSF. Additionally, some characteristic parameters for the PSF are provided, such as the equivalent bandwidth and the Strehl ratio. Comparing the PSF obtained using the fast Fourier transform algorithm of an optical system with known, detailed parameters to the analytical solution derived in this paper using only the typical parameters, the root mean square errors of the two methods were found to be less than 3% in the weak and medium defocus range. The attractive advantages of the universal model, which is independent of design details, objective types, and applications, are discussed.

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Section: Introductionmentioning

confidence: 99%

Analytical Model of Point Spread Function under Defocused Degradation in Diffraction-Limited Systems: Confluent Hypergeometric Function

Song,

Chen,

Tang

et al. 2024

Photonics

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“…Among these techniques, point spread function (PSF) engineering is a commonly employed approach. Some progress has been made in the research of 3D location in microregion by PSF engineering technology. − PSF engineering is a technique that encodes the fluorescence to be measured using devices such as a phase mask or spatial light modulator. The shape of PSF spots varies with the defocusing distance of the light source, along with conveying axial depth information .…”

Section: Introductionmentioning

confidence: 99%

Combining CdTe/CdS/ZnS Quantum Dot Fluorescence Temperature Measurements with Double-Helix Point Spread Function Axial Location for Intracellular 3D Thermograms

Jiang,

Zhu,

Yang

et al. 2023

ACS Appl. Nano Mater.

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Intracellular three-dimensional (3D) thermal mapping is a valuable technical tool for answering fundamental scientific questions about cellular thermal behavior and subcellular diagnostic therapy. The best method for achieving intracellular 3D thermal imaging is to collect intracellular temperature response while also obtaining a 3D position for the corresponding location. However, temperature measurement in cell space has not been accurately characterized due to the difficulty in accurately locating the 3D position in microregions. In this article, we developed a QD-based intracellular 3D thermal imaging system by combining QD fluorescence thermometry with double-helical point spread function (DH-PSF)-based axial localization. Liposome transfection was used to achieve the cellular uptake of CdTe/CdS/ZnS QDs in HOS cells. Five QDs’ temperatures with spatial distribution in living cells were realized in the axial range of 1.35 μm by collecting the thermosensitive fluorescence emission of discrete QDs and the axial imaging localization information, and their 3D thermal image distribution was mapped. Measurements and analysis show that the combination of QD fluorescence thermometry and axial localization of DH-PSF enables the acquisition of thermal image information in intracellular 3D microregions. Using this system, temperature variations at 3D locations in living cells exposed to ionomycin Ca2+ stress were also monitored. The method described in this article can be applied broadly to the study of intracellular space thermal response and thermal regulation.

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“…尽管基于DH-PSF的3D SPT具有很好的应用效果, 但成像深度仅有2 μm左右, 限制了其在完整细胞中的进一步应用, 需要设计新的大深度编码的PSF. 2013年, Li等 [19] 在经过优化的DH-PSF 的基础上利用同时具有DH-PSF调制与变形光栅成像功能的复合相位板, 进一步扩展了原有DH-PSF系统的探测深度, 构建了一套大景深无扫描纳米分辨三维多分子示踪成像系统(DDCM), 其三维定位精度达到5.3, 6.3和17.4 nm, 成像深度达到14 μm, 但该方法受光栅高阶衍射和色差的影响, 需要光栅色差补偿校正元件, 能量利用率低且系统相对复杂. 2021年, Li等 [20] (z-splitter prism) [21] And the effective DOF of the system is extended to 6 μm.…”

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Multifocus double-helix point spread function microscopy for 3D single particle tracking

Ma,

Gong,

Nie

et al. 2024

Acta Phys. Sin.

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Double-helix point spread function (DH-PSF) microscopy allows for nanoscale three-dimensional single particle tracking (3D SPT) and is commonly used in life sciences and other fields. However, its imaging depth-of-field (DOF) and localization accuracy are limited, which restricts its application in thick samples in vivo. To address this issue, this paper proposes a z-splitter prism-based multifocus DH-PSF microscopy (ZPMDM) method and system to improve the DOF and localization accuracy of DH-PSF microscopy without scanning. It solves the problem of large DOF detection of 3D SPT in whole living cells. By means of systematic calibration, the average 3D localization accuracies of the three channels of ZPMDM were determined to be σleft(x, y, z)=(4.4 nm, 4.6 nm, 10.5 nm), σmiddle(x, y, z)=(4.3 nm, 4.2 nm, 8.2 nm), and σright(x, y, z)=(4.8 nm, 4.4 nm, 10.3 nm). And the effective DOF of the system was extended to 6 μm. Furthermore, the ZPMDM system was used to track fluorescent microspheres in a glycerol-water mixture across a large depth-of-field range. The Brownian motion of the fluorescent microspheres in the mixture solution was also investigated. The experimental results demonstrate that the errors between the experimentally obtained diffusion coefficients and the theoretical diffusion coefficients are all within 10%. The reliability of the ZPMDM system in achieving single-particle 3D tracking imaging is verified by this study. The validity of the method was further verified by preliminarily investigating the phagocytosis phenomenon of live macrophages. This is significant for the development and application of nanoscale 3D SPT.

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Particles 3D tracking with large axial depth by using the 2π-DH-PSF

Cited by 10 publications

References 17 publications

Analytical Model of Point Spread Function under Defocused Degradation in Diffraction-Limited Systems: Confluent Hypergeometric Function

Analytical Model of Point Spread Function under Defocused Degradation in Diffraction-Limited Systems: Confluent Hypergeometric Function

Combining CdTe/CdS/ZnS Quantum Dot Fluorescence Temperature Measurements with Double-Helix Point Spread Function Axial Location for Intracellular 3D Thermograms

Multifocus double-helix point spread function microscopy for 3D single particle tracking

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