Particles Sorting in Micro Channel Using Designed Micro Electromagnets of Magnetic Field Gradient

Chung, Yung-Chiang; Wu, Chen-Ming; Lin, Shiuan-Huei

doi:10.1016/j.jmmm.2016.01.075

Cited by 26 publications

(11 citation statements)

References 20 publications

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“…Acoustic waves radiated from two opposite directions interfere with each other and generate standing waves that create pressure points at specific points depending on the wavelength inside the linear microchannel [ 124 ]. As in acoustic-based resonators and electric field manipulators [ 7 , 125 ], magnetic field-based manipulators [ 126 , 127 ] and optical field-based transducers [ 8 , 128 ] could generate gradients inside the microchannel to manipulate microparticles or nanoparticles for focus in a specific point, sorting in a specific path, and purifying to increase the density inside a content to be used, Figure 10 .…”

Section: Microfluidicsmentioning

confidence: 99%

Biomedical Applications of Microfluidic Devices: A Review

et al. 2022

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Both passive and active microfluidic chips are used in many biomedical and chemical applications to support fluid mixing, particle manipulations, and signal detection. Passive microfluidic devices are geometry-dependent, and their uses are rather limited. Active microfluidic devices include sensors or detectors that transduce chemical, biological, and physical changes into electrical or optical signals. Also, they are transduction devices that detect biological and chemical changes in biomedical applications, and they are highly versatile microfluidic tools for disease diagnosis and organ modeling. This review provides a comprehensive overview of the significant advances that have been made in the development of microfluidics devices. We will discuss the function of microfluidic devices as micromixers or as sorters of cells and substances (e.g., microfiltration, flow or displacement, and trapping). Microfluidic devices are fabricated using a range of techniques, including molding, etching, three-dimensional printing, and nanofabrication. Their broad utility lies in the detection of diagnostic biomarkers and organ-on-chip approaches that permit disease modeling in cancer, as well as uses in neurological, cardiovascular, hepatic, and pulmonary diseases. Biosensor applications allow for point-of-care testing, using assays based on enzymes, nanozymes, antibodies, or nucleic acids (DNA or RNA). An anticipated development in the field includes the optimization of techniques for the fabrication of microfluidic devices using biocompatible materials. These developments will increase biomedical versatility, reduce diagnostic costs, and accelerate diagnosis time of microfluidics technology.

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Section: Microfluidicsmentioning

confidence: 99%

Biomedical Applications of Microfluidic Devices: A Review

et al. 2022

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“…Small spherical magnetic beads—usually consisting of a paramagnetic core embedded in a non-magnetic matrix, such as a polymer or quartz [1,2]—are widely used in bioanalysis and medical applications [2,3,4,5,6]. These applications include separating target molecules from background molecules [7,8,9], investigating molecular interactions inside living cells [5,10,11], directing neuronal migration and growth [12], detecting specific proteins and DNA sequences in plasma [13,14,15,16], and reducing background in opto-chemical sensing [17]. Many of these applications require accurate manipulation of the magnetic beads on the nano and micro scales.…”

Section: Introductionmentioning

confidence: 99%

Configuration and Design of Electromagnets for Rapid and Precise Manipulation of Magnetic Beads in Biosensing Applications

2019

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Rapid and precise manipulation of magnetic beads on the nano and micro scales is essential in many biosensing applications, such as separating target molecules from background molecules and detecting specific proteins and DNA sequences in plasma. Accurately moving magnetic beads back and forth requires at least two adjustable magnetic field gradients. Unlike permanent magnets, electromagnets are easy to design and can produce strong and adjustable magnetic field gradients without mechanical motion, making them desirable for use in robust and safe medical devices. However, using multiple magnetic field sources to manipulate magnetic beads presents several challenges, including overlapping magnetic fields, added bulk, increased cost, and reduced durability. Here, we provide a thorough analysis, including analytical calculations, numerical simulations, and experimental measurements, of using two electromagnets to manipulate magnetic beads inside a miniature glass cell. We analyze and experimentally demonstrate different aspects of the electromagnets’ design, such as their mutual influence, the advantages and disadvantages of different pole tip geometries, and the correlation between the electromagnets’ positions and the beads’ aggregation during movement. Finally, we have devised a protocol to maximize the magnetic forces acting on magnetic beads in a two-electromagnet setup while minimizing the electromagnets’ size. We used two such electromagnets in a small footprint magnetic modulation biosensing system and detected as little as 13 ng/L of recombinant Zika virus antibodies, which enables detection of Zika IgM antibodies as early as 5 days and as late as 180 days post symptoms onset, significantly extending the number of days that the antibodies are detectable.

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“…The counting of the tagged cells was repeated and consistent results within ±1% deviation was seen. When applied 1A electric current, they separated 2.8 µm magnetic beads from 6.0 µm polystyrene particles with efficiency of 95% [212]. Distinctive from these reports, we implemented microelectromagnets for live cell separation.…”

Section: Sorting Of Magnetically Tagged Hela Cellsmentioning

confidence: 99%

“…Chung et al fabricated a microelectromagnets by electroplating 5 µm copper on glass substrate. They used this microelectromagnets to separate 2.8 µm magnetic beads from polystyrene particles with separation efficiency of 95% [212]. However, these devices did not prove their feasibilities by separating live cells.…”

Section: Single Striplinementioning

confidence: 99%

“…On the other hand, the continuous-flow separation mode possesses many important merits, such as high throughput, the possibility of continuous monitoring and high potential for system integration[34]. Although there were a number of microfluidic cell manipulation devices that exploited microelectromagnets, only a few of them worked in continue-flow mode[210][211][212]. As the prominent advantage of microelectromagnetsbased cell sorter is that the magnetic field can be tuned to control cell isolation, it is necessary investigate the cell separation performance under various electric current.…”

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confidence: 99%

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Microfluidic devices for cell separation and sample concentration

Liu¹

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Microfluidic platforms for cell separation and downstream bio-sample analysis provide powerful tools for biomedical fundamental research and practical applications. Therefore, the overall goal of my dissertation is to develop such integrative platforms using novel techniques.We first develop a magnetofluidic device using microelectromagnets for magnetic field generation and applied it for cancer cell separation. The cell separation efficiency can reach up to 79 % using 1A electric current. This tunable device would be a promising platform to separate many types of cells. Next, we propose a hybrid size and affinity-based multiplexed cell sorter, consisting spiral channel, inertial alignment segment and magnetic separation part. This is the first platform integrating spiral inertial microfluidics and magnetophoretic separation technique in one single chip. Using blood cell sample, a good separation performance (>90% efficiency) is achieved. This novel hybrid platform offers a promising alternative to many existing cell separation technologies.Sample concentration technology is another critical aspect of biomedical innovation. Utilizing our developed fabrication techniques, we establish paper-based microfluidic platforms using ion concentration polarization effect for bio-sample concentration. The sample concentration performances are comparable with other reported results. Our paper-based platforms and fabrication techniques hold great promise for creating simple and inexpensive paper-based assays, which can be used for practical point-of-care (POC) diagnostics.

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Particles Sorting in Micro Channel Using Designed Micro Electromagnets of Magnetic Field Gradient

Cited by 26 publications

References 20 publications

Biomedical Applications of Microfluidic Devices: A Review

Biomedical Applications of Microfluidic Devices: A Review

Configuration and Design of Electromagnets for Rapid and Precise Manipulation of Magnetic Beads in Biosensing Applications

Microfluidic devices for cell separation and sample concentration

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