Partition Chromatography on Ion Exchange Resins. Separation of Sugars.

Samuelson, Olof; Swenson, Birgit; Lukkari, Sakari; Block-Bolten, A.; Toguri, J. M.; Flood, H.

doi:10.3891/acta.chem.scand.16-2056

Cited by 22 publications

(6 citation statements)

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“…On both cation-exchange and anion-exchange styrene-divinylbenzene resins, the retention of non-ionic polar compounds was observed to increase with increasing concentration of ethanol in ethanol-water mobile phases [40,41]. These early low-efficient, time-consuming, but surprisingly highly selective separations were later reviewed [42].…”

Section: Ion Exchange and Zwitterionic Stationary Phasesmentioning

confidence: 99%

Stationary phases for hydrophilic interaction chromatography, their characterization and implementation into multidimensional chromatography concepts

Jandera

2008

J of Separation Science

227

144

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Hydrophilic interaction chromatography (HILIC) is becoming increasingly popular for separation of polar samples on polar columns in aqueous-organic mobile phases rich in organic solvents (usually ACN). Silica gel with decreased surface concentration of silanol groups, or with chemically bonded amino-, amido-, cyano-, carbamate-, diol-, polyol-, or zwitterionic sulfobetaine ligands are used as the stationary phases for HILIC separations, in addition to the original poly(2-sulphoethyl aspartamide) strong cation-exchange HILIC material. The type of the stationary and the composition of the mobile phase play important roles in the mixed-mode HILIC retention mechanism and can be flexibly tuned to suit specific separation problems. Because of excellent mobile phase compatibility and complementary selectivity to RP chromatography, HILIC is ideally suited for highly orthogonal 2-D LC-LC separations of complex samples containing polar compounds, such as peptides, proteins, oligosaccharides, drugs, metabolites and natural compounds. This review attempts to present an overview of the HILIC separation systems, possibilities for their characterization and emerging HILIC applications in 2-D off-line and on-line LC-LC separations of various samples, in combination with RP and other separation modes.

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Section: Ion Exchange and Zwitterionic Stationary Phasesmentioning

confidence: 99%

Stationary phases for hydrophilic interaction chromatography, their characterization and implementation into multidimensional chromatography concepts

Jandera

2008

J of Separation Science

227

144

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“…Sugar stationary phases that were prepared using the click chemistry approach were reported as effective materials for HILIC mode separations 43–49. Other types of HILIC stationary phases consist of organic polymer materials such as both cation‐exchange and anion‐exchange styrene–divinylbenzene resins 50, 51; zwitterionic stationary phases such as sulfobetaine 23, 52–54 and phosphorycholine‐bonded phases 55, silica‐based monolithic columns 56–58 and organic polymer HILIC monolithic columns 59, 60 were also reported.…”

Section: Introductionmentioning

confidence: 99%

Sulfonated cyclofructan 6 based stationary phase for hydrophilic interaction chromatography

Padivitage

Armstrong

2011

J of Separation Science

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A stationary phase composed of silica-bonded sulfonated cyclofructan 6 (SCF6) was synthesized and evaluated for hydrophilic interaction chromatography (HILIC). The separation of a large variety of polar compounds was evaluated on different versions of the stationary phase and compared with the same separations obtained with commercially available HILIC columns. The new columns successfully separate polar and hydrophilic compounds including β blockers, xanthines, salicylic acid related compounds, nucleic acid bases, nucleosides, maltooligosaccharides, water soluble vitamins and amino acids. The separation conditions were optimized by changing the composition and the pH of the mobile phase. The dependence of analyte retention on temperature was studied using van't Hoff plots. The newly synthesized stationary phase showed broad applicability for HILIC mode separations.

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“…With the meter in a horizontal position to minimize needle oscillation due to rocking of the boat, six analyses of the sample gave a mean value of 92 ppb NH3-N with a standard deviation of 3 ppb. Other samples were acidified to pH [3][4] with 6M HC1 to stabilize the ammonia and analyzed the same day in the laboratory by the electrode and also by the phenolhypochlorite method (4). The results of triplicate analyses with both methods are listed in Table along with similar results for marine life display tank water analyses.…”

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confidence: 94%

“…They have been discussed by Harris and Habgood (7). One of these which is particularly adaptable for use with a digital computer is that proposed by Rowan (2,3). This method, however, suffers from the fact that it does not take proper account of the void volume of the column.…”

mentioning

confidence: 99%

“…A treatment is here presented which will take account of the void volume in substantially all cases when packed columns are used and many cases in which capillary columns are employed. It will at the same time allow the use of certain techniques of calculation reported previously (3) which are especially well suited to use with a digital computer. Of course, direct numerical integration, which is the simplest method, can (and generally must) be carried out by means of a computer.…”

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confidence: 99%

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Calculations in programmed temperature gas chromatography when void volume is not negligible. New approach

Rowan¹,

Leach²

1973

Anal. Chem.

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Partition Chromatography on Ion Exchange Resins. Separation of Sugars.

Cited by 22 publications

References 0 publications

Stationary phases for hydrophilic interaction chromatography, their characterization and implementation into multidimensional chromatography concepts

Stationary phases for hydrophilic interaction chromatography, their characterization and implementation into multidimensional chromatography concepts

Sulfonated cyclofructan 6 based stationary phase for hydrophilic interaction chromatography

Calculations in programmed temperature gas chromatography when void volume is not negligible. New approach

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