Partition of tocopheryl glucopyranoside into liposome membranes studied by fluorescence methods

Synergistic and antagonistic effects between alpha-tocopherol and phenolic acids in liposome system: spectroscopic study

“…Presence in vicinity of the other compound leads to changes in microenvironment of the DPH resulting in shortening of fluorescence lifetime proportional to degree of interactions between compounds and membrane structure. The shortest lifetime was noted for DPH in the presence of α-T (5.80 ns), which indicates that α-T incorporates near to hydrophobic part of PC membrane as shown in the other studies [18,19,24]. Among studied phenolic acids, the shortest fluorescence lifetime of DPH was obtained for sample containing FA.…”

“…3. It shows the highest PCF values for α-T very probably due to the fact that α-T was embedded into the membrane interior [18,19,24], where effectively protects unsaturated fatty acids against oxidation. The PCF values of α-T were directly proportional to α-T concentration.…”

“…According to our earlier studies [18,19], we assumed that α-T resides inside the hydrophilic phase of the membrane, whereas the phytyl chain is anchored in the hydrocarbon core. Such location of α-T has already been indicated also by other authors [20].…”

Liposomal delivery systems for anti-cancer analogues of vitamin E

“…Association of α-tocopheryl glucopyranoside (α-TOG) with phosphatidylcholine (PC) bilayers positioned the chromanol head of α-TOG into the hydrophilic area by electrostatic interaction with choline nitrogen. Bulky hydrophilic sugar unit protruding above PC bilayer was surrounded by water molecules of the aqueous phase [66]. Structural modification of α-TOH either with non-polar or polar groups generates VE analogues having different incorporation within PL bilayer.…”

Molecular organization of perylene derivatives in Langmuir–Blodgett multilayers

“…Steady-state fluorescence spectra of the LB films were recorded by a homemade spectrofluorimeter [32] with a single photon detection system based on H6240-02 photon counting head from Hamamatsu (Japan). Fluorescence lifetimes were measured using the time-correlated single photon counting technique carried out with TimeHarp 100 PC-board, as it was described in [33] with the exception that the excitation source was a sub-nanosecond pulsed LED diode with maximum emission centered at 450 nm, powered by a PDL 800-D driver. The emission was detected by a PMA 182 photon sensor head (all the instruments from PicoQuant, Germany).…”