2013
DOI: 10.1021/jp404135g
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Partitioning of Oleic Acid into Phosphatidylcholine Membranes Is Amplified by Strain

Abstract: Partitioning of fatty acids into phospholipid membranes is studied on giant unilamellar vesicles (GUVs) utilizing phase-contrast microscopy. With use of a micropipet, an individual GUV is transferred from a vesicle suspension in a mixed glucose/sucrose solution into an isomolar glycerol solution with a small amount of oleic acid added. Oleic acid molecules intercalate into the phospholipid membrane and thus increase the membrane area, while glycerol permeates into the vesicle interior and thus via osmotic infl… Show more

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Cited by 24 publications
(20 citation statements)
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“…Two scenarios are forecast by our model: one where vesicles are spherical when extruded, and one where they are deflated by 5% when extruded, as generally observed experimentally2427. The Supplementary Material details the vesicle population mixing procedure used to initialise our theoretical model, adjusting it to realistic experimental conditions.…”
Section: Resultsmentioning
confidence: 90%
See 1 more Smart Citation
“…Two scenarios are forecast by our model: one where vesicles are spherical when extruded, and one where they are deflated by 5% when extruded, as generally observed experimentally2427. The Supplementary Material details the vesicle population mixing procedure used to initialise our theoretical model, adjusting it to realistic experimental conditions.…”
Section: Resultsmentioning
confidence: 90%
“…the concentration of lipid in the surrounding medium), the area of surface available for absorption S μ and function u , based on the dimensionless reduced surface The reduced surface encodes the surface area to volume ratio of a vesicle, when the latter internal volume is considered as a sphere: Φ = 1 denotes a vesicle perfectly spherical in shape, whereas Φ < 1 or Φ > 1 indicates a vesicle in osmotic tension or deflated respectively. Taking this into consideration, we define u as the following conditional function to denote that lipid uptake is only increased when the the bilayer is stressed24. Flaccid vesicles do not have extra enhancement of lipid uptake rate.…”
Section: Theoretical Model Of Vesicle Competitionmentioning
confidence: 99%
“…42 Furthermore, OA was added to modify the fluidity of LNCs and permeabilize cell membrane for enhanced LNCcell interaction and intestinal transport. 43,44 The reduction in heating-cooling range during LNC formation could be explained by OA-induced fluidization of the surfactant monolayer 45 and partial incorporation of some OA molecules into the surfactant film of the microemulsion phase. 46 MFS was incorporated for enhancing LNC structural integrity and imparting membrane activity.…”
Section: Discussionmentioning
confidence: 99%
“…30,50 Efficacy of MFS-LNCs against both mature and prepatent S. mansoni worms in S. mansoni-infected mice in a single oral 20 mg/kg dose was mainly attributed to facilitation of intestinal transport and interaction with the tegument surface, an effect significantly augmented by inclusion of OA as gut membrane permeabilizer 52 and/or fusogenic agent. 43 More recently, MFS was shown to unmask parasite surface antigens not normally exposed to the host during infection, enhancing their serological recognition. 53 In the present study, inclusion of MFS and OA in PZQ-OA-MFS-LNCs did not significantly enhance antischistosomal activity (Tables 2 and 3), probably because of the lower MFS dose used (10 mg/kg) and masking of OA effects by Span 80 (sorbitan monoleate), reported to exert a fusogenic effect on phospholipid vesicles.…”
Section: Discussionmentioning
confidence: 99%
“…In general the system's chemical potential depends on the membrane's elastic energy, which changes due to insertion of hydrophobic molecules. 10,34 In our case we have to take into account the elastic energy (eqn (1)) and gravitational energy (eqn (2)) and also (a) the entropic mixing of LPS molecules with POPC molecules, expressed in the approximation for low molar ratios of LPS in the membrane (the ideal gas limit), (b) the interactions between the intercalated LPS molecules and the lipid molecules in the membrane, and (c) the free energy of LPS molecules in the solution. 35 These contributions to the free energy (F) can be expressed by the sequence of terms as:…”
Section: Equilibrium Partitioning Of Lps Into the Membranementioning
confidence: 99%