Parvicella tangerina gen. nov., sp. nov. (Parvicellaceae fam. nov., Flavobacteriales), first cultured representative of the marine clade UBA10066, and Lysobacter luteus sp. nov., from activated sludge of a seawater-processing wastewater treatment plant

Abstract

Two strains isolated from a sample of activated sludge that was obtained from a seawater-based wastewater treatment plant on the southeastern Mediterranean coast of Spain have been characterized to achieve their taxonomic classification, since preliminary data suggested they could represent novel taxa. Given the uniqueness of this habitat, as this sort of plants are rare in the world and this one used seawater to process an influent containing intermediate products from amoxicillin synthesis, we also explored their ecology and the annotations of their genomic sequences. Analysis of their 16S rRNA gene sequences revealed that one of them, which was orange-pigmented, was distantly related to Vicingus serpentipes (family Vicingaceae ) and to other representatives of neighbouring families in the order Flavobacteriales (class Flavobacteriia ) by 88–89 % similarities; while the other strain, which was yellow-pigmented, was a putative new species of Lysobacter (family Xanthomonadaceae , order Xanthomonadales , class Gammaproteobacteria ) with Lysobacter arseniciresistens as closest relative (97.3 % 16S rRNA sequence similarity to its type strain). Following a polyphasic taxonomic approach, including a genome-based phylogenetic analysis and a thorough phenotypic characterization, we propose the following novel taxa: Parvicella tangerina gen. nov., sp. nov. (whose type strain is AS29M-1T=CECT 30217T=LMG 32344T), Parvicellaceae fam. nov. (whose type genus is Parvicella), and Lysobacter luteus sp. nov. (whose type strain is AS29MT=CECT 30171T=LMG 32343T).

Meta-omic profiling reveals ubiquity of genes encoding for the nitrogen-rich biopolymer cyanophycin in activated sludge microbiomes

“…PHOS-HE28 have been identified in activated sludge and are related to bacteria isolated from saline environments, primarily seawater ( Bowman, 2020 ). PHOS-HE28 and other related bacteria possess a ppk1 gene, so it is possible that these bacteria can store phosphate like PAO ( Lucena et al, 2022 ), though more examination of ppk1 phylogeny and other phosphate transport genes is necessary to infer this function. Further investigation of PHOS-HE28 may be a promising avenue for integrating cyanophycin accumulation into existing treatment facilities given its ubiquity in activated sludge.…”

Meta-omic profiling reveals ubiquity of genes encoding for the high value nitrogen rich biopolymer cyanophycin in activated sludge microbiomes

“…PHOS-HE28 have been identified in activated sludge and are related to bacteria isolated from saline environments (Bowman, 2020). PHOS-HE28 and other related bacteria possess a ppk1 gene, so it is possible that these bacteria can store polyphosphate (Lucena et al, 2022), though more examination of ppk1 phylogeny and other phosphate transport genes is necessary to infer this function. Further investigation of PHOS-HE28 may be a promising avenue for integrating cyanophycin accumulation into existing treatment facilities given its ubiquity in numerous activated sludge microbiomes.…”

Coralliovum pocilloporae gen. nov., sp. nov. and Sanyastnella coralliicola gen. nov., sp. nov. isolated from coral tissue: proposal of two new families, Coralliovaceae fam. nov. and Sanyastnellaceae fam. nov

“…The order Flavobacteriales is a deep phylogenetic lineage of the class Flavobacteriia. At the time of writing, it consists of eight families, Blattabacteriaceae, Crocinitomicaceae, Cryomorphaceae, Flavobacteriaceae, Ichthyobacteriaceae, Parvicellaceae, Schleiferiaceae and Weeksellaceae [9][10][11][12][13][14][15]. The present study was designed to determine the taxonomic positions of two isolates recovered from tissue of the coral Pocillopora damicornis.…”