pasilla, the Drosophila Homologue of the Human Nova-1 and Nova-2 Proteins, Is Required for Normal Secretion in the Salivary Gland

Seshaiah, Partha; Miller, Bret; Myat, Monn Monn; Andrew, Deborah J.

doi:10.1006/dbio.2001.0429

Cited by 40 publications

(47 citation statements)

References 39 publications

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“…jim is also involved in chromatin silencing (Mugat et al 2015) and PIWIinteracting RNA processes in follicle cells (Saito et al 2009). Finally, ps is spliced sex specifically (Telonis-Scott et al 2009) and is a splicing factor (Seshaiah et al 2001) and Vmat is involved in transmembrane transport as a splice variant that is involved in dopamine, serotonin, and octopamine transport (Greer et al 2005). Both dopamine signaling and octopamine signaling are implicated in mediating the postmating response (Rezaval et al 2014;Landis et al 2015).…”

Section: Cis-and Trans-effectsmentioning

confidence: 99%

Buffering of Genetic Regulatory Networks inDrosophila melanogaster

et al. 2016

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Regulatory variation in gene expression can be described by cis-and trans-genetic components. Here we used RNA-seq data from a population panel of Drosophila melanogaster test crosses to compare allelic imbalance (AI) in female head tissue between mated and virgin flies, an environmental change known to affect transcription. Indeed, 3048 exons (1610 genes) are differentially expressed in this study. A Bayesian model for AI, with an intersection test, controls type I error. There are 200 genes with AI exclusively in mated or virgin flies, indicating an environmental component of expression regulation. On average 34% of genes within a cross and 54% of all genes show evidence for genetic regulation of transcription. Nearly all differentially regulated genes are affected in cis, with an average of 63% of expression variation explained by the cis-effects. Trans-effects explain 8% of the variance in AI on average and the interaction between cis and trans explains an average of 11% of the total variance in AI. In both environments cis-and trans-effects are compensatory in their overall effect, with a negative association between cis-and trans-effects in 85% of the exons examined. We hypothesize that the gene expression level perturbed by cis-regulatory mutations is compensated through trans-regulatory mechanisms, e. g., trans and cis by trans-factors buffering cis-mutations. In addition, when AI is detected in both environments, cis-mated, cis-virgin, and trans-mated-trans-virgin estimates are highly concordant with 99% of all exons positively correlated with a median correlation of 0.83 for cis and 0.95 for trans. We conclude that the gene regulatory networks (GRNs) are robust and that trans-buffering explains robustness.

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Section: Cis-and Trans-effectsmentioning

confidence: 99%

Buffering of Genetic Regulatory Networks inDrosophila melanogaster

et al. 2016

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“…α-PH4α-SG1 (E.W.A. and D.J.A., unpublished), α-Pasilla (Seshaiah et al, 2001) and α-DSC73 (D.J.A., unpublished) are polyclonal antisera made in rat. α-En is a polyclonal antibody made in rabbit that cross-reacts with unknown secretory products in the salivary gland (Myat and Andrew, 2002).…”

Section: In Situ Hybridization and Antibody Stainingmentioning

confidence: 99%

CrebA regulates secretory activity in theDrosophilasalivary gland and epidermis

2005

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Understanding how organs acquire the capacity to perform their respective functions is important for both cell and developmental biology. Here, we have examined the role of early-expressed transcription factors in activating genes crucial for secretory function in the Drosophila salivary gland. We show that expression of genes encoding proteins required for ER targeting and translocation, and proteins that mediate transport between the ER and Golgi is very high in the early salivary gland. This high level expression requires two early salivary gland transcription factors; CrebA is required throughout embryogenesis and Fkh is required only during late embryonic stages. As Fkh is required to maintain late CrebA expression in the salivary gland, Fkh probably works through CrebA to affect secretory pathway gene expression. In support of these regulatory interactions, we show that CrebA is important for elevated secretion in the salivary gland. Additionally, CrebA is required for the expression of the secretory pathway genes in the embryonic epidermis, where CrebA had previously been shown to be essential for cuticle development. We show that zygotic mutations in several individual secretory pathway genes result in larval cuticle phenotypes nearly identical to those of CrebA mutants. Thus, CrebA activity is linked to secretory function in multiple tissues.

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“…As embryogenesis proceeds, the salivary gland lumen continues to fill with electron-dense secreted products and lumen width increases uniformly throughout the length of the lumen (Myat and Andrew, 2002;Seshaiah et al, 2001). Secretory function of salivary gland cells is controlled by pasilla (ps) which encodes a Drosophila homologue of the human Nova family RNA-binding proteins that function in RNA splicing (Jensen et al, 2000;Seshaiah et al, 2001), and by PH4 SG1 and PH4 SG2, which encode homologues of the -subunit of resident endoplasmic reticulum enzymes that hydroxylate proline in select secreted proteins (Abrams et al, 2006;Kivirikko and Pihlajaniemi, 1998 ). In ps mutant salivary glands, secretory contents within the lumen and apical vesicles is reduced, and the lumen fails to expand uniformly (Seshaiah et al, 2001).…”

Section: Control Of Salivary Gland Lumen Size Through Secretory Activitymentioning

confidence: 99%

“…Secretory function of salivary gland cells is controlled by pasilla (ps) which encodes a Drosophila homologue of the human Nova family RNA-binding proteins that function in RNA splicing (Jensen et al, 2000;Seshaiah et al, 2001), and by PH4 SG1 and PH4 SG2, which encode homologues of the -subunit of resident endoplasmic reticulum enzymes that hydroxylate proline in select secreted proteins (Abrams et al, 2006;Kivirikko and Pihlajaniemi, 1998 ). In ps mutant salivary glands, secretory contents within the lumen and apical vesicles is reduced, and the lumen fails to expand uniformly (Seshaiah et al, 2001). Similar to ps mutant embryos, PH4 SG1 and PH4 SG2 mutant embryos have reduced secretory products in the salivary gland lumens and are characterized by abnormally shaped lumens with regions of expansion, constriction and closure (Abrams et al, 2006).…”

Section: Control Of Salivary Gland Lumen Size Through Secretory Activitymentioning

confidence: 99%