Passive Protection of Mice Against Lethal Francisella Tularensis (Live Tularemia Vaccine Strain) Infection by the Sera of Human Recipients of the Live Tularemia Vaccine

Drabick, Joseph J.; Narayanan, R. B.; Williams, Jim C.; LeDuc, James W.; Nacy, Carol A.

doi:10.1097/00000441-199408000-00003

Cited by 68 publications

(66 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our previous results have demonstrated that serum (10,35) or monoclonal anti-LVS antibodies (33) transfer only very weak protection against lethal LVS challenge under limited experiment circumstances. We believe that it is highly unlikely that LPS-stimulated protection is mediated by specific antibodies for a number of reasons.…”

Section: Discussionmentioning

confidence: 99%

Purified Lipopolysaccharide fromFrancisella tularensisLive Vaccine Strain (LVS) Induces Protective Immunity against LVS Infection That Requires B Cells and Gamma Interferon

2000

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Purified Lipopolysaccharide fromFrancisella tularensisLive Vaccine Strain (LVS) Induces Protective Immunity against LVS Infection That Requires B Cells and Gamma Interferon

2000

View full text Add to dashboard Cite

show abstract

“…injection of 100 l of xylazine (20 mg/ml) and ketamine (1 mg/ml). For sublethal infection, 10 3 CFU of LVS, and for lethal challenge, 10 4 CFU of LVS in 50 l of PBS, was delivered by the i.n. route.…”

Section: Mice and Bacteria Animal Inoculation Challenge And Adoptivmentioning

confidence: 99%

“…Cultures of LVS grown overnight in Mueller-Hinton broth were washed with PBS and adjusted to 10 9 CFU/ml in RPMI 1640. A total of 10 8 CFU of LVS (100 l) was incubated with 50 l of heat-inactivated normal or immune serum for 30 min on ice.…”

Section: Macrophage Infection Assaymentioning

confidence: 99%

Prophylactic and Therapeutic Use of Antibodies for Protection against Respiratory Infection with Francisella tularensis

Kirimanjeswara

Golden

Bakshi

et al. 2007

The Journal of Immunology

103

142

View full text Add to dashboard Cite

The role of Abs in protection against respiratory infection with the intracellular bacterium Francisella tularensis is not clear. To investigate the ability of Abs to clear bacteria from the lungs and prevent systemic spread, immune serum was passively administered i.p. to naive mice before intranasal F. tularensis live vaccine strain infection. It was found that immune serum treatment provided 100% protection against lethal challenge while normal serum or Ig-depleted immune serum provided no protection. Protective efficacy was correlated with increased clearance of bacteria from the lung and required expression of FcγR on phagocytes, including macrophages and neutrophils. However, complement was not required for protection. In vitro experiments demonstrated that macrophages were more readily infected by Ab-opsonized bacteria but became highly efficient in killing upon activation by IFN-γ. Consistent with this finding, in vivo Ab-mediated protection was found to be dependent upon IFN-γ. SCID mice were not protected by passive Ab transfer, suggesting that T cells but not NK cells serve as the primary source for IFN-γ. These data suggest that a critical interaction of humoral and cellular immune responses is necessary to provide sterilizing immunity against F. tularensis. Of considerable interest was the finding that serum Abs were capable of conferring protection against lethal respiratory tularemia when given 24–48 h postexposure. Thus, this study provides the first evidence for the therapeutic use of Abs in Francisella-infected individuals.

show abstract

“…Much of the data regarding F. tularensis immunopathogenesis, as well as the mechanism of protection afforded by the vaccine, comes from murine models. Humoral immunity was previously believed to be important as passive protection against LVS challenge was demonstrated in mice given immune serum from LVS-vaccinated humans (Drabick et al, 1994). However, vaccine-induced humoral responses to LVS may play no role in protection against human pathogenic F. tularensis strains (Tarnvik, 1989).…”

Section: Subject Termsmentioning

confidence: 99%

Transcriptome analysis of human immune responses following live vaccine strain (LVS) Francisella tularensis vaccination

Fuller

Brittingham

Porter³

et al. 2007

Molecular Immunology

View full text Add to dashboard Cite

The live vaccine strain (LVS) of Francisella tularensis is the only vaccine against tularemia available for humans, yet its mechanism of protection remains unclear. We probed human immunological responses to LVS vaccination with transcriptome analysis using PBMC samples from volunteers at time points pre-and post-vaccination. Gene modulation was highly uniform across all time points, implying commonality of vaccine responses. Principal components analysis revealed three highly distinct principal groupings: pre-vaccination (−144 h), early (+18 and +48 h), and late postvaccination (+192 and +336 h). The most significant changes in gene expression occurred at early post-vaccination time points (≤48 h), specifically in the induction of pro-inflammatory and innate immunity-related genes. Evidence supporting modulation of innate effector function, specifically antigen processing and presentation by dendritic cells, was especially apparent. Our data indicate that the LVS strain of F. tularensis invokes a strong early response upon vaccination. This pattern of gene regulation may provide insightful information regarding both vaccine efficacy and immunopathogenesis that may provide insight into infection with virulent strains of F. tularensis. Additionally, we obtained valuable information that should prove useful in evaluation of vaccine lots as well as efficacy testing of new anti-F. tularensis vaccines.

show abstract

Passive Protection of Mice Against Lethal Francisella Tularensis (Live Tularemia Vaccine Strain) Infection by the Sera of Human Recipients of the Live Tularemia Vaccine

Cited by 68 publications

References 8 publications

Purified Lipopolysaccharide fromFrancisella tularensisLive Vaccine Strain (LVS) Induces Protective Immunity against LVS Infection That Requires B Cells and Gamma Interferon

Purified Lipopolysaccharide fromFrancisella tularensisLive Vaccine Strain (LVS) Induces Protective Immunity against LVS Infection That Requires B Cells and Gamma Interferon

Prophylactic and Therapeutic Use of Antibodies for Protection against Respiratory Infection with Francisella tularensis

Transcriptome analysis of human immune responses following live vaccine strain (LVS) Francisella tularensis vaccination

Contact Info

Product

Resources

About