The study aimed to determine the effects of red clover (Trifolium pratense) silage with high phyto-oestrogen content on ewe performance during their first breeding season. Red clover silage containing formononetin, biochanin A, genistein, and daidzein was fed to 10 nulliparous ewes of the prolific Finnish Landrace breed before, during and after the breeding season, for a total of 5 months. A control group of 10 ewes was fed with grass silage. The mean numbers of foetuses per pregnancy were 2.1 ± 0.7 and 2.2 ± 0.8 for the red clover and control groups, respectively. The total mass of the uterus with its contents was significantly greater in ewes of the red clover group compared with those of the control group. This difference was mainly explained by the greater volume of foetal fluids. Serum progesterone concentration in the red clover group was significantly lower over the entire period analysed than in the control group. In conclusion, the fecundity of the ewes was not reduced by red clover feed with high phyto-oestrogen concentrations. The volume of foetal fluids increased that could increase the risk for vaginal prolapse before the term.Keywords: ewe, red clover, phyto-oestrogen, formononetin, equol
ImplicationsThe symbiotic rhizobia bacteria in red clover can fix atmospheric nitrogen, which promotes the growth of red clover itself and subsequent crops. This ability makes red clover valuable crops, especially in organic agriculture. Red clover is also a valuable ruminant feed, for example, lambs fed with red clover grow very well. Red clover is a rich source of polyphenols such as isoflavones, also referred to as phyto-oestrogens. Phyto-oestrogens can cause infertility problems in animals. As organic farming and the use of red clover is ever increasing, it is important to study what effect red clover feeding has on ewes' reproduction.