Patchouli virus X, a new potexvirus from <i>Pogostemon clabin</i>

Filho, P. E. Meissner; Resende, Renato O.; Lima, M. I.; Kitajima, E. W.

doi:10.1111/j.1744-7348.2002.tb00218.x

Cited by 10 publications

(5 citation statements)

References 15 publications

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“…Earlier preliminary work has been done on viral diseases of patchouli except in three reports; Patchouli Virus X [3] , Patchouli mild mosaic virus ( PaMMV ) [4] and PStV [5] where the virus was identified at the species level and partially or fully sequenced. The remaining studies were disease reports, three from India [11] , [12] , [13] .…”

Section: Discussionmentioning

confidence: 99%

“…Some viruses naturally infecting patchouli have already been completely or partially characterized ( Table 1 ). Patchouli virus X ( PatVX ) [3] , Patchouli mild mosaic virus ( PaMMV ) [4] and Peanut stripe virus ( PStV) [5] have been characterized and sequenced. There are other reports in which viruses either have been characterized at the preliminary level or published as disease reports; e.g., potexvirus [6] , [7] , patchouli mottle virus ( PaMoV ) [4] , Pepper ring spot virus ( PRV ) [8] , Patchouli mosaic virus ( PaMV ) [9] , Tobacco necrosis virus ( TNV ) - a presumed Rhabdovirus [10] and yellow mosaic of Patchouli [11] , [12] , [13] .…”

Section: Introductionmentioning

confidence: 99%

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Serological and Molecular Studies of a Novel Virus Isolate Causing Yellow Mosaic of Patchouli [Pogostemon cablin (Blanco) Benth]

et al. 2013

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Here we have identified and characterized a devastating virus capable of inducing yellow mosaic on the leaves of Patchouli [Pogostemon cablin (Blanco) Benth]. The diagnostic tools used were host range, transmission studies, cytopathology, electron microscopy, serology and partial coat protein (CP) gene sequencing. Evidence from biological, serological and sequence data suggested that the causal virus belonged to genus Potyvirus, family Potyviridae. The isolate, designated as Patchouli Yellow Mosaic Virus (PaYMV), was transmitted through grafting, sap and the insect Myzus persicae (Sulz.). Flexuous rod shaped particles with a mean length of 800 nm were consistently observed in leaf-dip preparations from natural as well as alternate hosts, and in purified preparation. Cytoplasmic cylindrical inclusions, pinwheels and laminar aggregates were observed in ultra-thin sections of infected patchouli leaves. The purified capsid protein has a relative mass of 43 kDa. Polyclonal antibodies were raised in rabbits against the coat protein separated on SDS – PAGE; which were used in ELISA and western blotting. Using specific antibodies in ELISA, PaYMV was frequently detected at patchouli plantations at Lucknow and Bengaluru. Potyvirus-specific degenerate primer pair (U335 and D335) had consistently amplified partial CP gene from crude preparations of infected tissues by reverse transcription polymerase chain reaction (RT-PCR). Comparison of the PCR product sequence (290 bp) with the corresponding regions of established potyviruses showed 78–82% and 91–95% sequence similarity at the nucleotide and amino acid levels, respectively. The results clearly established that the virus under study has close homology with watermelon mosaic virus (WMV) in the coat protein region and therefore could share a common ancestor family. Further studies are required to authenticate the identity of PaYMV as a distinct virus or as an isolate of WMV.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Serological and Molecular Studies of a Novel Virus Isolate Causing Yellow Mosaic of Patchouli [Pogostemon cablin (Blanco) Benth]

et al. 2013

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“…CMV pada tanaman nilam baru ditemukan di Indonesia. Di luar negeri, virus yang menginfeksi tanaman nilam adalah dari genus Potyvirus (Natsuaki et al 1994;Singh et al 2009;Zaim et al 2013), Fabavirus (Natsuaki et al 1994) dan Potexvirus (Filho et al 2002). Hasil penelitian ini merupakan laporan pertama karakterisasi molekuler CMV asal nilam, baik di Indonesia maupun di luar negeri.…”

Section: Perunutan Nukleotida Dan Analisis Sekuenunclassified

IDENTIFIKASI MOLEKULER CUCUMBER MOSAIC VIRUS (CMV) ASAL TANAMAN NILAM (Pogostemon cablin) / Molecular Identification of Cucumber Mosaic Virus Infecting Patchouli (Pogostemon cablin)

Miftakhurohmah

Nyana

Damayanti

et al. 2017

j. penelitian tanam. industri

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Penyakit mosaik pada tanaman nilam di Indonesia berasosiasi dengan infeksi Telosma mosaic virus (TeMV), Broad bean wilt virus 2 (BBWV2), Cymbidium mosaic virus (CymMV) dan Cucumber mosaic virus (CMV). TeMV, BBWV2 dan CymMV sudah diidentifikasi secara molekuler, sedangkan CMV baru terdeteksi secara serologi. Karakte risasi molekuler setiap virus diperlukan sebagai salah satu dasar pengambilan tindakan pengendalian. Penelitian ini bertujuan untuk mengidentifikasi CMV asal tanaman nilam secara molekuler berdasarkan sekuen nukleotida gen CP. Tiga sampel daun nilam bergej ala terinfeksi virus diambil dari koleksi tanaman nilam di rumah kaca Balittro. Sampel daun diekstraksi asam nukleat totalnya (RNA+DNA). Asam nukleat total diamplifikasi dengan teknik one step reverse transcriptase polymerase chain reaction (RT-PCR) menggunakan primer spesifik gen coat protein (CP) CMV. Produk PCR berukuran 650 pb (pasang basa) dirunut sekuen nukleotidanya serta dianalisis homologi dan hubungan filogenetikanya dengan sekuen isolat-isolat CMV yang ada di GenBank. Sekuen nukleotida dan asam amino gen CP CMV asal nilam menunjukkan persentase kemiripan terbesar (97,1 dan 97,7%) dengan isolat um -Jepang. Analisis filogeni menunjukkan bahwa CMV asal nilam berkerabat sangat dekat dengan CMV isolat um-Jepang dengan nilai bootstrap 100%, dan berada dalam satu kelompok dengan isolat-isolat CMV subgrup IB. Keberadaan CMV subgrup IB pada tanaman nilam perlu diwaspadai karena subgrup I lebih virulen dibandingkan subgrup II. Penelitian ini merupakan laporan pertama karakterisasi molekuler CMV nilam, baik di Indonesia maupun di luar negeri, yang dapat digunakan sebagai salah satu dasar pengambilan tindakan pengendalian. Kata kunci: analisis homologi, pohon filogeni, nukleotida, asam amino ABSTRACT Mosaic disease on Indonesian patchouli associated with i nfection of TeMV, BBWV2, CymMV and CMV. TeMV, BBWV2 and CymMV has been identified molecularly, while CMV just was detected serologically. The objective of this study was to identify CMV from patchouli by molecular approach based on CP gene nucleotide sequence Leaf samples were collected from three mosaic symptomatic patchouli plants in greenhouse of Balittro. Leaf samples were extracted for the total nucleic acids (RNA + DNA). Nucleic acids were amplified using specific primer for CP gene of CMV by one step RT-PCR technique. The DNA of PCR product with the size of ~ 650 bp was directly sequenced and analyzed for its homology with sequences of CMV isolates extracted from Gene Bank. CMV CP gene from patchouli showed the highest of

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“…Genus Potexvirus pada nilam di Brazil diidentifikasi sebagai virus baru, yang dinamakan dengan PatVX (Filho et al, 2002). Namun demikian, peneliti dari Brazil tersebut hanya melakukan pembuatan antiserum, uji serologi baik dengan antiserum dan dengan uji protein, tanpa melakukan sekuensing.…”

Section: Cymbidium Mosaic Virusunclassified

Identifikasi Molekuler Broad Bean Wilt Virus 2 (Bbwv2) Dan Cymbidium Mosaic Virus (Cymmv) Asal Tanaman Nilam (Pogostemon Cablin Benth.)

Miftakhurohmah

Suastika

Damayanti

et al. 2016

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

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Molecular identification Broad Bean Wilt Virus 2 (BBWV2) and Cymbidium Mosaic Virus (CymMV) from patchouli plant (Pogostemon cablin Benth.). Several viruses have been reported to be associated with mosaic disease on patchouli plant in Indonesia. This study aims to identify the two viruses in patchouli cultivation in West Java by studying the molecular characterization. Mosaic symptomatic leaf samples taken from patchouli cultivation in Manoko (Bandung Barat District, West Java Province). RNA extraction was performed using Xprep Plant RNA mini kit. RNA amplification with RT-PCR technique using primers for the cp gene region of BBWV2 and CymMV. The PCR product was sent to PT. Science Genetics Indonesia to do sequencing, then analyzed nucleotide sequences. Results of RT-PCR were performed successfully obtained DNA bands with size accordance with the predictions of the primer design for BBWV2 and CymMV cp region. Further, based on nucleotide and amino acid sequence analyses, the two virus isolates were confirmed as BBWV2 and CymMV respectively. Phylogenetic analyses revealed that BBWV2 Manoko clustered with BBWV2 from Singapore (original host of Brazilian red-cloak), China (pepper) and South Korea (chili). Whereas, CymMV Manoko become one cluster with CymMV from India (Phaius sp.), Indonesia (Dendrobium), China (vanilla), Thailand (Oncidium), Hawai (Dendrobium) and South Korea (Cymbidium).

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Patchouli virus X, a new potexvirus from Pogostemon clabin

Cited by 10 publications

References 15 publications

Serological and Molecular Studies of a Novel Virus Isolate Causing Yellow Mosaic of Patchouli [Pogostemon cablin (Blanco) Benth]

Serological and Molecular Studies of a Novel Virus Isolate Causing Yellow Mosaic of Patchouli [Pogostemon cablin (Blanco) Benth]

IDENTIFIKASI MOLEKULER CUCUMBER MOSAIC VIRUS (CMV) ASAL TANAMAN NILAM (Pogostemon cablin) / Molecular Identification of Cucumber Mosaic Virus Infecting Patchouli (Pogostemon cablin)

Identifikasi Molekuler Broad Bean Wilt Virus 2 (Bbwv2) Dan Cymbidium Mosaic Virus (Cymmv) Asal Tanaman Nilam (Pogostemon Cablin Benth.)

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