Paternal factors in recurrent pregnancy loss: an insight through analysis of non-synonymous single-nucleotide polymorphism in human testis-specific chaperone HSPA2 gene

Mohanty, Gayatri; Jena, Srikanta; Kar, S.; Samanta, Luna

doi:10.1007/s11356-021-17799-3

Cited by 6 publications

(3 citation statements)

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“…The most relevant studies in human samples have reported reduced levels of HSPA2 expression in samples from patients suffering varicocele [41,42], elevated ROS (reactive oxygen species) levels [43], infertility [44], or disability to bind to the ZP [45]. Moreover, a recent report demonstrates the association between lower HSPA2 expression with recurrent pregnancy loss, indicating the potential usefulness of HSPA2 in the diagnosis and prognosis of paternal effects [46].…”

Section: Introductionmentioning

confidence: 99%

Molecular Chaperone HSPA2 Distribution During Hyaluronic Acid Selection in Human Sperm

et al. 2022

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During fertilization, sperm hyaluronidase activity is essential for spermatozoa to successfully penetrate the hyaluronic acid-enriched extracellular matrix of the cumulus cells. Since molecular chaperones, as the heat shock protein A2, are typically involved in bringing hyaluronic acid receptors to the cell surface, here we evaluated the presence and spatial location of HSPA2 on human spermatozoa based on its hyaluronic acid binding capacity. This study included 16 normozoospermic sperm samples from volunteering donors. The location of HSPA2 was studied in cells before and after 1-h incubation under capacitating conditions, as well as in spermatozoa selected according to their ability of binding to hyaluronic acid. Our results showed no significant differences in HSPA2 immunofluorescent cells before and after 1 h of incubation in capacitating conditions. Nevertheless, after hyaluronic acid selection, the percentage of HSPA2-labelled cells increased significantly, indicating that the interaction with hyaluronic acid may induce the unmasking of HSPA2 epitopes. Furthermore, after swim-up and hyaluronic acid selection, spermatozoa presented a highly immunostained equatorial band with a homogeneous fluorescence throughout the acrosomal region. This distribution has been previously suggested to have important implications in male fertility. Noteworthy, a homogeneous fluorescence among the acrosomal region with a more intense labelling at the apical region was observed only in hyaluronic acid bound sperm cells, which may be associated with primary gamete recognition. Our findings suggest that the hyaluronic acid selection technique and HSPA2 biomarker should be considered candidates to complement the classic seminal analysis before recommending an appropriate assisted reproduction technique.

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Section: Introductionmentioning

confidence: 99%

Molecular Chaperone HSPA2 Distribution During Hyaluronic Acid Selection in Human Sperm

et al. 2022

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“…The genes whose mutations have been associated with the reduction of male fertility include methylenetetrahydrofolate reductase (MTHFR), which is important in DNA methylation in stem cells ( 294 ), steroid 5-alpha reductase responsible for the conversion of testosterone into dihydrotestosterone (DHT), i.e. a more active metabolite ( 295 ), protamines replacing histone proteins during the condensation of the nuclear chromatin of sperm ( 296 ), heat shock protein A2 (HSPA2) responsible for the protection of cells from exposure to stress factors ( 269 ).…”

Section: Testesmentioning

confidence: 99%

Endocrine disorders and fertility and pregnancy: An update

et al. 2023

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It is estimated that more and more couples suffer from fertility and pregnancy maintenance disorders. It is associated with impaired androgen secretion, which is influenced by many factors, ranging from genetic to environmental. It is also important to remember that fertility disorders can also result from abnormal anatomy of the reproductive male and female organ (congenital uterine anomalies – septate, unicornuate, bicornuate uterus; acquired defects of the uterus structure – fibroids, polyps, hypertrophy), disturbed hormonal cycle and obstruction of the fallopian tubes resulting from the presence of adhesions due to inflammation, endometriosis, and surgery, abnormal rhythm of menstrual bleeding, the abnormal concentration of hormones. There are many relationships between the endocrine organs, leading to a chain reaction when one of them fails to function properly. Conditions in which the immune system is involved, including infections and autoimmune diseases, also affect fertility. The form of treatment depends on infertility duration and the patient’s age. It includes ovulation stimulation with clomiphene citrate or gonadotropins, metformin use, and weight loss interventions. Since so many different factors affect fertility, it is important to correctly diagnose what is causing the problem and to modify the treatment regimen if necessary. This review describes disturbances in the hormone secretion of individual endocrine organs in the context of fertility and the maintenance of pregnancy.

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“…Targeting structural proteins and interactions shows promise but requires inhibitor development. Although progress has been made in identifying biomarkers for infertility (Mohanty et al, 2020;Jena et al, 2021;Mohanty et al, 2021;Nayak et al, 2023), druggable targets remain elusive. This special volume covers recent OMICS-based advancements in understanding reproduction at molecular level not only in human but also in farm animals including the application of artificial intelligence in infertility diagnostics.Karanwal et al studied the significance of water buffalo (Bubalus bubalis) in India's dairy sector and the economic losses from failed pregnancies after artificial insemination (AI), often due to low-quality bull semen.…”

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confidence: 99%

Editorial: Omics for infertility and contraception: two sides of same coin

Jena,

Mohanty,

Kesari

et al. 2023

Front. Cell Dev. Biol.

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Editorial on the Research Topic Omics for infertility and contraception: two sides of same coinThe Research Topic entitles "Omics for infertility and contraception: two sides of same coin" is focused on the intertwined issues of contraception and infertility. Contraception is essential for population stability, while infertility poses emotional and psychological challenges for the workingage population. There is a significant global need for family planning (57% of women), but millions lack access (United Nations et al., 2019). Conversely, about 15% of couples face infertility (Eshre, 2017). Assisted reproductive technologies (ART) offer hope but have limitations due to our limited understanding of reproductive physiology. Addressing these challenges requires prioritizing nonhormonal contraceptives (non-HPG) for both genders to avoid side effects, such as cancer risks associated with hormonal contraceptives (Hemmerling et al., 2020). It is also crucial to identify specific molecules responsible for reproductive functions. These interconnected issues benefit from advancements in each other. Additionally, OMICS technologies and bioinformatics enable comprehensive study of cellular processes from DNA to metabolites, offering strategies for non-HPG contraceptives (Johnston and Goldberg, 2020). Targeting structural proteins and interactions shows promise but requires inhibitor development. Although progress has been made in identifying biomarkers for infertility (Mohanty et al., 2020;Jena et al., 2021;Mohanty et al., 2021;Nayak et al., 2023), druggable targets remain elusive. This special volume covers recent OMICS-based advancements in understanding reproduction at molecular level not only in human but also in farm animals including the application of artificial intelligence in infertility diagnostics.Karanwal et al. studied the significance of water buffalo (Bubalus bubalis) in India's dairy sector and the economic losses from failed pregnancies after artificial insemination (AI), often due to low-quality bull semen. They used LC-MS/MS to analyze high fertile (HF) and low fertile (LF) buffalo sperm proteins. They found 1,385 proteins, with 1,002 shared and 288 unique to HF, and 95 to LF. HF had 211 significantly more abundant proteins, linked to vital sperm functions. In contrast, LF had 342 less abundant proteins associated with processes like glycolysis and inflammation. Fertility-related proteins (e.g., AKAP3, Sp17, and DLD) were validated, offering potential markers for buffalo fertility prediction, aiding the farming sector's economic stability.

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Paternal factors in recurrent pregnancy loss: an insight through analysis of non-synonymous single-nucleotide polymorphism in human testis-specific chaperone HSPA2 gene

Cited by 6 publications

References 46 publications

Molecular Chaperone HSPA2 Distribution During Hyaluronic Acid Selection in Human Sperm

Molecular Chaperone HSPA2 Distribution During Hyaluronic Acid Selection in Human Sperm

Endocrine disorders and fertility and pregnancy: An update

Editorial: Omics for infertility and contraception: two sides of same coin

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