Paternal
            <scp>RNA</scp>
            contributions in the
            <i>Caenorhabditis elegans</i>
            zygote

Stoeckius, Marlon; Grün, Dominic; Rajewsky, Nikolaus

doi:10.15252/embj.201488117

Cited by 64 publications

(55 citation statements)

References 56 publications

(75 reference statements)

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“…This study, as well as previous studies (Alcazar et al, 2008;Ashe et al, 2012;Grishok et al, 2000;Luteijn et al, 2012;Shirayama et al, 2012;Stoeckius et al, 2014), clearly demonstrates that mutator-dependent silencing information inherits through both the paternal and maternal lineages. It is, however, not clear how this memory is precisely transmitted.…”

Section: Parental Memory Of Rnaesupporting

confidence: 83%

Maternal piRNAs Are Essential for Germline Development following De Novo Establishment of Endo-siRNAs in Caenorhabditis elegans

2015

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The Piwi-piRNA pathway represents a germline-specific transposon-defense system. C. elegans Piwi, prg-1, is a non-essential gene and triggers a secondary RNAi response that depends on mutator genes, endo-siRNAs (22G-RNAs), and the 22G-RNA-binding Argonaute protein HRDE-1. Interestingly, silencing of PRG-1 targets can become PRG-1 independent. This state, known as RNAe, is heritable and depends on mutator genes and HRDE-1. We studied how the transgenerational memory of RNAe and the piRNA pathway interact. We find that maternally provided PRG-1 is required for de novo establishment of 22G-RNA populations, especially those targeting transposons. Strikingly, attempts to re-establish 22G-RNAs in absence of both PRG-1 and RNAe memory result in severe germline proliferation defects. This is accompanied by a disturbed balance between gene-activating and -repressing 22G-RNA pathways. We propose a model in which CSR-1 prevents the loading of HRDE-1 and in which both PRG-1 and HRDE-1 help to keep mutator activity focused on the proper targets.

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Section: Parental Memory Of Rnaesupporting

confidence: 83%

Maternal piRNAs Are Essential for Germline Development following De Novo Establishment of Endo-siRNAs in Caenorhabditis elegans

2015

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“…Work in model organism Caenorhabditis elegans has identified hundreds of paternally derived mRNA present in the 1-cell embryo, before initiation of transcription (Stoeckius et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Paternal obesity in a rodent model affects placental gene expression in a sex-specific manner

et al. 2015

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Fetal growth restriction (FGR) is a major obstetric complication stemming from poor placental development. We have previously demonstrated that paternal obesity in mice is associated with impaired embryo development and significantly reduced fetal and placental weights. We hypothesised that the FGR observed in our rodent model of paternal diet-induced obesity is associated with alterations in metabolic, cell signalling and stress pathways. Male C57BL/6 mice were fed either a normal or high-fat diet for 10 weeks before sperm collection for IVF and subsequent embryo transfer. On embryonic day 14, placentas were collected and RNA extracted from both male and female placentas to assess mRNA expression of 24 target genes using custom RT-qPCR arrays. Peroxisome proliferator-activated receptor alpha (Ppara) and caspase-12 (Casp12) expression were significantly altered in male placentas from obese fathers compared with normal (P!0.05), but not female placentas. PPARA and CASP12 proteins were localised within the placenta to trophoblast giant cells by immunohistochemistry, and relative protein abundance was determined by western blot analysis. DNA was also extracted from the same placentas to determine methylation status. Global DNA methylation was significantly increased in female placentas from obese fathers compared with normal (P!0.05), but not male placentas. In this study, we demonstrate for the first time that paternal obesity is associated with changes in gene expression and methylation status of extraembryonic tissue in a sex-specific manner. These findings reinforce the negative consequences of paternal obesity before conception, and emphasise the need for more lifestyle advice for prospective fathers.

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“…However, both coding and non-coding RNAs have been well characterized in sperm (Jodar et al 2013), and it has been revealed that sperm can carry diverse RNA species (including mRNAs and small RNAs, some of which are sperm specific) to the fertilized zygote (Ostermeier et al 2004, Stoeckius et al 2014, indicating that these RNAs may play a unique paternal role in the development of zygotes.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, pseudogene-derived piRNAs complementary to protein-coding genes, which may regulate the expression of their parent gene in the male germ line, have been identified in the human sperm (Krawetz et al 2011, Pantano et al 2015. Moreover, Stoeckius and coworkers found that approximately half of all assayed endogenous piRNAs and siRNAs of 1-cell embryos in Caenorhabditis elegans are of sperm origin (Stoeckius et al 2014). The enrichment of tRNA-derived small RNAs (tsRNAs) in the sperm was first described in mice (Peng et al 2012), and then extended to humans, rats and rabbits .…”

Section: Introductionmentioning

confidence: 99%

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Cai¹,

Wu²,

Shen³

et al. 2017

Reproduction

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The similarities and differences of small RNAs in seminal plasma, epididymal sperm and ejaculated sperm remain largely undefined. We conducted a systematic comparative analysis of small RNA profiles in pig ejaculated sperm, epididymal sperm and seminal plasma and found that the diversity distribution of small RNA species was generally similar, whereas the abundance of small RNAs is dramatically different across the three libraries; miRNAs and small RNAs derived from rRNA, tRNA, small nuclear RNA, 7SK RNA, NRON RNA and cis-regulatory RNA were enriched in the three libraries, but piRNA was absent. A large population of small RNAs from ejaculated sperm are ejaculated sperm specific, and only 8-30% of small RNAs overlapped with those of epididymal sperm or seminal plasma and a small proportion (5-18%) of small RNAs were shared in the three libraries, suggesting that, in addition to the testes, sperm RNAs may also originate from seminal plasma, epididymis as well as other resources. Most miRNAs were co-distributed but differentially expressed across the three libraries, with epididymal sperm exhibiting the highest abundance, followed by ejaculated sperm and seminal plasma. The prediction of target genes of the top 10 highly expressed miRNAs across the three libraries revealed that these miRNAs may be involved in spermatogenesis, zygote development and the interaction between the environment and animals. Our study provides the first description of the similarities and differences of small RNA profiles in ejaculated sperm, epididymal sperm and seminal plasma and indicates that sperm RNA may have origins other than the testes.Reproduction (2017) 153 [785][786][787][788][789][790][791][792][793][794][795][796]

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Paternal RNA contributions in the Caenorhabditis elegans zygote

Cited by 64 publications

References 56 publications

Maternal piRNAs Are Essential for Germline Development following De Novo Establishment of Endo-siRNAs in Caenorhabditis elegans

Maternal piRNAs Are Essential for Germline Development following De Novo Establishment of Endo-siRNAs in Caenorhabditis elegans

Paternal obesity in a rodent model affects placental gene expression in a sex-specific manner

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

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