Pathfinding during spinal tract formation in the chick-quail chimera analysed by species-specific monoclonal antibodies

Tanaka, Hideaki; Kinutani, Masayuki; Agata, Akemi; Takashima, Yoichiro; Obata, Kunihiko

doi:10.1242/dev.110.2.565

Cited by 51 publications

(2 citation statements)

References 22 publications

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“…To visualize the grafted quail cells in the chick otic epithelium and in both ganglia, mAb QN and QCPN monoclonal antibodies were used. QN immunoreaction binds to some membrane molecules of quail nervous tissues, but not to chick tissues, with this method being an excellent marker for tracing neural processes in chick/quail chimeric embryos [ 48 , 69 ] ( Figure 1 a–e). QN staining helps to identify quail grafted neurons in the acoustic and vestibular ganglia from non-neuronal cells [ 71 ], and QCPN immunoreaction staining is useful to visualize the nuclei of quail transplanted cells in the developing central nervous system and inner ear [ 5 , 70 , 72 ].…”

Section: Resultsmentioning

confidence: 99%

“…To localize the cell body of quail transplanted cells in the chimeric embryos, QCPN (DSHB; 1/100) mAb antibodies were used, while QN (1/10, a kind gift from Dr. Tanaka; [ 69 ]) was used to visualize the neuronal processes. These antibodies were visualized using sheep anti-mouse (1/100; Jackson ImmunoResearch) and mouse-PAP (1/200; Jackson ImmunoResearch) antibodies.…”

Section: Methodsmentioning

confidence: 99%

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Origin of Neuroblasts in the Avian Otic Placode and Their Distributions in the Acoustic and Vestibular Ganglia

Hidalgo‐Sánchez

Callejas-Marín

Puelles

et al. 2023

Biology

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The inner ear is a complex three-dimensional sensorial structure with auditory and vestibular functions. This intricate sensory organ originates from the otic placode, which generates the sensory elements of the membranous labyrinth, as well as all the ganglionic neuronal precursors. How auditory and vestibular neurons establish their fate identities remains to be determined. Their topological origin in the incipient otic placode could provide positional information before they migrate, to later segregate in specific portions of the acoustic and vestibular ganglia. To address this question, transplants of small portions of the avian otic placode were performed according to our previous fate map study, using the quail/chick chimeric graft model. All grafts taking small areas of the neurogenic placodal domain contributed neuroblasts to both acoustic and vestibular ganglia. A differential distribution of otic neurons in the anterior and posterior lobes of the vestibular ganglion, as well as in the proximal, intermediate, and distal portions of the acoustic ganglion, was found. Our results clearly show that, in birds, there does not seem to be a strict segregation of acoustic and vestibular neurons in the incipient otic placode.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Origin of Neuroblasts in the Avian Otic Placode and Their Distributions in the Acoustic and Vestibular Ganglia

Hidalgo‐Sánchez

Callejas-Marín

Puelles

et al. 2023

Biology

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Pelvic plexus contributes ganglion cells to the hindgut enteric nervous system

Nagy¹,

Brewer²,

Mwizerwa³

et al. 2006

Developmental Dynamics

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The hindgut enteric nervous system (ENS) contains cells originating from vagal and sacral neural crest. In avians, the sacral crest gives rise to the nerve of Remak (NoR) and pelvic plexus. Whereas the NoR has been suggested to serve as the source of sacral crest-derived cells to the gut, the contribution of the pelvic ganglia is unknown. The purpose of this study was to test the hypothesis that the pelvic ganglia contribute ganglion cells to the hindgut ENS. We observed that the quail pelvic plexus develops from neural crest-derived cells that aggregate around the cloaca at embryonic day 5. Using chick-quail tissue recombinations, we found that hindgut grafts did not contain enteric ganglia unless the pelvic plexus was included. Neurofibers extended from the NoR into the intestine, but no ganglion cell contribution from the NoR was identified. These results demonstrate that the pelvic plexus, and not the NoR, serves as the staging area for sacral crest-derived cells to enter the avian hindgut, confirming the evolutionary conservation of this important embryologic process. Developmental Dynamics 236:73-83, 2007.

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Immunophenotypic characterization of enteric neural crest cells in the developing avian colorectum

Nagy¹,

Burns²,

Goldstein³

2012

Developmental Dynamics

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Background The enteric nervous system (ENS) develops from neural crest-derived cells that migrate along the intestine to form two plexuses of neurons and glia. While the major features of ENS development are conserved across species, minor differences exist, especially in the colorectum. Given the embryologic and disease-related importance of the distal ENS, the aim of this study was to characterize the migration and differentiation of enteric neural crest cells (ENCCs) in the colorectum of avian embryos. Results Using normal chick embryos and vagal neural tube transplants from GFP-transgenic chick embryos, we find ENCCs entering the colon at embryonic day (E) 6.5, with colonization complete by E8. Undifferentiated ENCCs at the wavefront express HNK-1, N-cadherin, Sox10, p75, and L1CAM. By E7, differentiation begins in the proximal colon, with L1CAM and Sox10 becoming restricted to neuronal and glial lineages, respectively. By E8, multiple markers of differentiation are expressed along the entire colorectum. Conclusions Our results establish the pattern of ENCC migration and differentiation in the chick colorectum, demonstrate the conservation of marker expression across species, highlight a range of markers, including neuronal cell adhesion molecules, which label cells at the wavefront, and provide a framework for future studies in avian ENS development.

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Pathfinding during spinal tract formation in the chick-quail chimera analysed by species-specific monoclonal antibodies

Cited by 51 publications

References 22 publications

Origin of Neuroblasts in the Avian Otic Placode and Their Distributions in the Acoustic and Vestibular Ganglia

Origin of Neuroblasts in the Avian Otic Placode and Their Distributions in the Acoustic and Vestibular Ganglia

Pelvic plexus contributes ganglion cells to the hindgut enteric nervous system

Immunophenotypic characterization of enteric neural crest cells in the developing avian colorectum

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