“…Examples of such software are AperioImageScope (Leica Biosystems), HCImage Hamamatsu software (Hamamatsu Photonics), and OptraASSAYS (OptraScan). On the other hand, there are closed-source platforms developed by third parties, e.g., Image-Pro (Media Cybernetics), APP Center (Visio Pharm), Aiforia, Pathobox, 30 and Halo image analysis platform (Indicalab). Since all of these are closed architectures, it is impossible to add functions as an external user, and most of them are too expensive for researchers, students, or small laboratories.…”