Pathogenesis of mucosal biofilm infections: challenges and progress

Dongari-Bagtzoglou, Anna

doi:10.1586/14787210.6.2.201

Cited by 96 publications

(89 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Indeed, their in vitro model for biofilm development by Pneumocystis carinii and Pneumocystis murina enabled prolonged growth of the organisms in vitro compared with planktonic culture conditions (Cushion et al 2009). Finally, mucosal infections, such as oropharyngeal candidiasis or vaginitis, are caused by true biofilms in which tissue, rather than a device, serves as the substrate for biofilm growth (Dongari-Bagtzoglou 2008;. In these situations, the biofilm-associated phenotype of drug resistance has not been shown.…”

mentioning

confidence: 99%

Fungal Biofilms, Drug Resistance, and Recurrent Infection

Desai

Mitchell

Andes

2014

Cold Spring Harbor Perspectives in Medicine

208

148

View full text Add to dashboard Cite

A biofilm is a surface-associated microbial community. Diverse fungi are capable of biofilm growth. The significance of this growth form for infection biology is that biofilm formation on implanted devices is a major cause of recurrent infection. Biofilms also have limited drug susceptibility, making device-associated infection extremely difficult to treat. Biofilm-like growth can occur during many kinds of infection, even when an implanted device is not present. Here we summarize the current understanding of fungal biofilm formation, its genetic control, and the basis for biofilm drug resistance.

show abstract

mentioning

confidence: 99%

Fungal Biofilms, Drug Resistance, and Recurrent Infection

Desai

Mitchell

Andes

2014

Cold Spring Harbor Perspectives in Medicine

208

148

View full text Add to dashboard Cite

show abstract

“…Biofilm can be defined as a microbial community well organized in the EPS that adheres to living or inanimate surfaces (1). Several gram-positive bacteria, gram-negative bacteria, and fungi of clinical significance can form biofilms.…”

Section: Discussionmentioning

confidence: 99%

“…A biofilm can be defined as a well-organized microbial community in the extracellular polymeric substance (EPS) that adheres to living or inanimate surfaces (1). Owing to their resistance to antibiotics and antiphagocytic effects, biofilms can frequently cause persistent chronic infections that are difficult to treat.…”

Section: Introductionmentioning

confidence: 99%

The comparison of various disinfectants’ efficacy on Staphylococcus aureus and Pseudomonas aeruginosa biofilm layers

Köse¹,

Yapar²

2017

Turk J Med Sci

View full text Add to dashboard Cite

IntroductionA biofilm can be defined as a well-organized microbial community in the extracellular polymeric substance (EPS) that adheres to living or inanimate surfaces (1). Owing to their resistance to antibiotics and antiphagocytic effects, biofilms can frequently cause persistent chronic infections that are difficult to treat. Biofilms are significant causes of morbidity and mortality (2,3). At least 65% of all bacterial infections are associated with biofilm (2,3). Infections such as natural valve endocarditis, otitis media, chronic bacterial prostatitis, cystic fibrosis, and periodontitis develop as a result of biofilms that form on living surfaces. The medical devices on which biofilms can develop include prosthetic heart valves, central venous catheters, urinary catheters, contact lenses, and intrauterine devices (4). Due to the high rates of mortality and morbidity associated with biofilms, several studies have been conducted on antimicrobials and particularly on the effectiveness of antibiotics against biofilms. It has been demonstrated that antibiotics show limited efficacy against biofilms as the biofilm layer persists. For this reason, it should be borne in mind that disinfectants serve as a significant alternative against human mucosa and biofilms on the surfaces of medical device (5). This study aims at investigating the effectiveness of orthophthalaldehyde, peracetic acid, hydrogen peroxide, and sodium hypochlorite against Pseudomonas aeruginosa and Staphylococcus aureus biofilm layers and the live microbial cells on the biofilm layers. Material and methods Bacterial strainsS. aureus (ATCC 6538) and P. aeruginosa (PA01) bacterial strains that are known to produce biofilms were selected (5,6). Bacterial cells stocked at -80 °C were passaged to a tryptic soy agar (TSA) plate (Merck). After being incubated for 24 h at 35-37 °C, a bacterial solution was Background/aim: Because biofilms are resistant to antibiotics and biocides, they usually cause chronic persistent infections, which are arduous to cure and have high mortality and morbidity. Our study aimed to investigate the efficiency of orthophthalaldehyde, peracetic acid, hydrogen peroxide, and sodium hypochlorite on Pseudomonas aeruginosa and Staphylococcus aureus biofilm layers and live microbial cells.Materials and methods: Biofilm layers were determined by crystal violet assay and live microbial cells were determined using a resazurin assay.Results: For Pseudomonas aeruginosa, sodium hypochlorite showed the most influential disinfection because it diminished 83.6% of the biofilm layer and decreased 99.7% of live microbial cells. For Staphylococcus aureus, hydrogen peroxide was determined the most active disinfectant with 80.3% reduction of the biofilm layer. Sodium hypochlorite was also determined to be the most efficient disinfectant with 99.8% reduction of live microbial cells. Sodium hypochlorite was the most influential disinfectant on biofilm layers and live microbial cells of both microorganisms. Conclusion:We concluded that if we use sodi...

show abstract

“…[1,3,4] Although the function of biofilms is unknown, their presence in necrotic bowel must have implications for the pathogenesis of bowel necrosis and for its medical and surgical management.…”

Section: Discussionmentioning

confidence: 99%

“…[3] Although biofilms have been detected in several mucosal locations, their ability to trigger human disease is still a matter of active investigation. [4] The pathogenic role of biofilms has been established for oral infections, chronic wounds, indwelling medical and surgical devices, and implants. [1,3,5] Currently, the link between gastrointestinal infections and biofilms in infants is less clear.…”

mentioning

confidence: 99%

Biofilms associated with bowel necrosis: A newly recognised phenomenon in infants

Brisighelli

Theron

et al. 2016

S Afr Med J

View full text Add to dashboard Cite

Background.A biofilm is defined as a collection of organisms attached to a surface and surrounded by a matrix. Objective. To present three cases in which bowel necrosis coexisted with biofilm. Methods. The medical records, bacteriological findings and tissue biopsies from three infants with bowel necrosis who subsequently died from sepsis were analysed. Tissue sent for histological evaluation was prepared for light microscopy. Haematoxylin and eosin (H&E), Sandiford and Alcian blue/periodic acid Schiff (ABPAS) stains were performed. Tissue samples were ex-waxed for electron microscopy in one case. Results. The three patients described all had necrotic bowel at laparotomy, all cultured Klebsiella pneumoniae from peritoneal pus swabs, and all died despite appropriate antibiotics. All specimens showed varying degrees of bowel necrosis and an organising acute peritoneal reaction. In addition, all showed colonies of Gram-negative bacteria within a mucopolysaccharide matrix. Conclusions. The identification of biofilms in necrotic bowel has raised questions regarding their clinical implications. Further studies are needed to evaluate all resected necrotic bowel for biofilms and the clinical implications of this finding.

show abstract

Pathogenesis of mucosal biofilm infections: challenges and progress

Cited by 96 publications

References 61 publications

Fungal Biofilms, Drug Resistance, and Recurrent Infection

Fungal Biofilms, Drug Resistance, and Recurrent Infection

The comparison of various disinfectants’ efficacy on Staphylococcus aureus and Pseudomonas aeruginosa biofilm layers

Biofilms associated with bowel necrosis: A newly recognised phenomenon in infants

Contact Info

Product

Resources

About