Pathogenesis of Mycobacterium spp. in zebrafish (Danio rerio) from research facilities

Watral, Virginia; Kent, Michael L.

doi:10.1016/j.cbpc.2006.06.004

Cited by 66 publications

(104 citation statements)

References 27 publications

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“…However, if infectious agents were responsible for the observed lesions, then both males and females would be affected, but in our study pathological alterations occurred only in females. Further, it has been reported that granulomatous and inflammatory changes in mycobacterial-infected zebrafish occur mainly in organs other than the ovaries (Watral & Kent 2007), which is in contrast to our fishes, where lesions occurred in the ovaries. All things considered, it is not likely that infectious agents were the cause of the ovarian changes in the control zebrafish; rather, we consider non-infective factors to be responsible.…”

Section: Abstract: Zebrafish · Gonad · Histopathologycontrasting

confidence: 86%

“…Although the inability to detect parasites, fungi or acid-fast bacteria argues against a primary inflammatory process as the cause of the observed lesions, we cannot fully exclude the possibility that infectious agents are involved. For instance, Watral & Kent (2007) pointed out that staining of acid-fast bacteria may remain negative even when molecular methods or bacterial cultures succeed in demonstrating the presence of mycobacteria. However, if infectious agents were responsible for the observed lesions, then both males and females would be affected, but in our study pathological alterations occurred only in females.…”

Section: Abstract: Zebrafish · Gonad · Histopathologymentioning

confidence: 99%

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Background pathology of the ovary in a laboratory population of zebrafish Danio rerio

Rossteuscher¹,

Schmidt‐Posthaus²,

Schäfers³

et al. 2008

Dis. Aquat. Org.

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Adult zebrafish Danio rerio originating from one stock used as control animals in a toxicological study were examined histopathologically for the occurrence of spontaneous lesions in the gonads. While no histopathological changes were seen in the testes, the ovaries showed lesions consisting mainly of acute granulomatous inflammation with increased atresia and the presence of egg debris in the ovarian parenchyma and in the oviduct. Since infectious agents could not be detected and the fish were not exposed to toxicants, we consider these lesions as spontaneous alterations of the ovaries

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Section: Abstract: Zebrafish · Gonad · Histopathologycontrasting

confidence: 86%

Section: Abstract: Zebrafish · Gonad · Histopathologymentioning

confidence: 99%

Background pathology of the ovary in a laboratory population of zebrafish Danio rerio

Rossteuscher¹,

Schmidt‐Posthaus²,

Schäfers³

et al. 2008

Dis. Aquat. Org.

View full text Add to dashboard Cite

show abstract

“…al., 1997;Sanders and Swaim, 2001;Kent et. al., 2004;Watral and Kent, 2006). Monitoring for the presence of mycobacteria in populations of fish has proven to be difficult, and the potential for low-level background infections is a common risk-factor, especially in large research facilities.…”

Section: Discussionmentioning

confidence: 99%

Mycobacterium marinum produces long-term chronic infections in medaka: A new animal model for studying human tuberculosis

Broussard¹,

Ennis²

2007

Comparative Biochemistry and Physiology Part C: Toxicology &

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Human infection by Mycobacterium tuberculosis is endemic, with approximately 2 billion infected and is the most common cause of adult death due to an infectious agent. Because of the slow growth rate of M. tuberculosis and risk to researchers, other species of Mycobacterium have been employed as alternative model systems to study human tuberculosis (TB). Mycobacterium marinum may be a good surrogate pathogen, conferring TB-like chronic infections in some fish. Medaka (Oryzias latipes) has been established for over five decades as a laboratory fish model for: toxicology, genotoxicity, teratogenesis, carcinogenesis, classical genetics and embryology. We are investigating if medaka might also serve as a host for M. marinum in order to model human TB. We show that both acute and chronic infections are inducible in a dose dependent manner. Colonization of target organs and systemic granuloma formation has been demonstrated through the use of histology. M. marinum expressing green fluorescent protein (Gfp) was used to monitor bacterial colonization of these organs in fresh tissues as well as in intact animals. Moreover, we have employed the SeeThrough fish line, a variety of medaka devoid of major pigments, to monitor real-time disease progression, in living animals. We have also compared the susceptibility of another prominent fish model, zebrafish (Danio rerio), to our medaka-M. marinum model. We determined the course of infections in zebrafish is significantly more severe than in medaka. Together, these results indicate that the medaka-M. marinum model provides unique advantages for studying chronic mycobacteriosis.

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“…Zebrafish are susceptible to a number of mycobacterial pathogens, of which M. marinum is the most interesting candidate (Watral and Kent 2007). M. marinum naturally inhabits aquatic environments and is the causative agent of a tuberculosis-like disease in coldblooded animals (Tobin and Ramakrishnan 2008).…”

Section: General Properties Of Zebrafishmentioning

confidence: 99%

Animal Models of Tuberculosis: Zebrafish

Leeuwen

Sar

Bitter

2014

Cold Spring Harbor Perspectives in Medicine

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Correspondence: a.vandersar@vumc.nl Over the past decade the zebrafish (Danio rerio) has become an attractive new vertebrate model organism for studying mycobacterial pathogenesis. The combination of mediumthroughput screening and real-time in vivo visualization has allowed new ways to dissect host pathogenic interaction in a vertebrate host. Furthermore, genetic screens on the host and bacterial sides have elucidated new mechanisms involved in the initiation of granuloma formation and the importance of a balanced immune response for control of mycobacterial pathogens. This article will highlight the unique features of the zebrafish-Mycobacterium marinum infection model and its added value for tuberculosis research.

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Pathogenesis of Mycobacterium spp. in zebrafish (Danio rerio) from research facilities

Cited by 66 publications

References 27 publications

Background pathology of the ovary in a laboratory population of zebrafish Danio rerio

Background pathology of the ovary in a laboratory population of zebrafish Danio rerio

Mycobacterium marinum produces long-term chronic infections in medaka: A new animal model for studying human tuberculosis

Animal Models of Tuberculosis: Zebrafish

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