Pathogenesis of urinary tract infections — Amoxicillin induces genital escherichia coli colonization

Herthelius, B M; Hedström, K.-G.; Möllby, R.; Nord, Carl Erik; Pettersson, L; Winberg, Jan

doi:10.1007/bf01645066

Cited by 27 publications

(11 citation statements)

References 21 publications

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“…This can be explained by the defense mechanism of these boys not being sufficiently developed to prevent recolonization. It is also possible that antibiotic treatment can decrease the host colonization resistance by disturbing the ecological balance in the periurethral region (18). The pattern was different among girls.…”

Section: Discussionmentioning

confidence: 99%

Spontaneous Clearance of Asymptomatic Bacteriuria in Infants

Wettergren

Jodal

1990

Acta Paediatrica

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In an unselected population of 3,581 infants, 14 girls and 36 boys were found to have bacteriuria verified by suprapubic aspiration. Among the bacteriuric infants, one girl and one boy developed symptoms of pyelonephritis close to the time of detection; the others remained asymptomatic. Eleven girls and 34 boys were left untreated. One of these girls and 7 of the boys became abacteriuric after treatment for respiratory tract infections. Ten girls and 26 boys became spontaneously abacteriuric, with a median persistence of bacteriuria of 2 months in girls and 1.5 months in boys. Recurrences were observed among boys only in those who had been treated with antibiotics.

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Section: Discussionmentioning

confidence: 99%

Spontaneous Clearance of Asymptomatic Bacteriuria in Infants

Wettergren

Jodal

1990

Acta Paediatrica

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“…All 16 monkeys were outbred. The procedure has been described in detail elsewhere (17,21,22). In summary, a catheter was introduced into the vagina, and 3 ml of an E. coli suspension (10 9 CFU/ml) with either DS17, DS17-8, or DS17-1 was flushed over the mucous membranes.…”

Section: Methodsmentioning

confidence: 99%

Induction of protective immunity after escherichia coli bladder infection in primates. Dependence of the globoside-specific P-fimbrial tip adhesin and its cognate receptor.

Söderhäll¹,

Normark²,

Ishikawa³

et al. 1997

J. Clin. Invest.

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Clinical observations suggest that immune mechanisms affect etiology and course of recurrent cystitis. A primate infection model was used to show that primary bladder infection with a uropathogenic P-fimbriated strain (binding to globoside present in the bladder wall) protects against rechallenge with homologous as well as heterologous Escherichia coli strains for up to 5-6 mo. In contrast, mutant derivatives producing P-fimbriae either lacking the tip adhesin protein or carrying an adhesin for which no bladder receptor was present, were unable to induce protection, even though they generated bladder infections of similar duration as the wild type. Therefore, the protective effect mediated by the adhesin seemed to depend upon the presence of its cognate receptor. Since the wild strain also mediated protection against mutants that lacked the adhesin, our data suggest that the globoside-binding PapG adhesin acts as an adjuvant during infection to enhance a specific response against other bacterial antigens. In fact, the globoside-binding strain DS17, but not the mutant DS17-1, unable to bind to membrane-bound globoside,

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“…This can be the reason for vaginal pH modifications and a subsequent alteration of the composition of the vaginal microflora in those women. 16) Some other studies have reported on modification of the normal microflora due to hormone treatment or influences: Herthelius et al 17) have suggested that the estrogens in monkeys and humans produce changes which would favor lactobacilli colonization of the vaginal tract. Marrie et al 18) have shown that the percentage of Lactobacillus colonization in women increases during menarche and pregnancy, and decreases after menopause.…”

Section: Discussionmentioning

confidence: 99%

Experimental Administration of Estradiol on the Colonization of Lactobacillus fermentum and Escherichia coli in the Urogenital Tract of Mice.

Ruíz

Rey

Holgado

et al. 2001

Biological & Pharmaceutical Bulletin

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The physiological condition of the urogenital tract changes during the life of different animals or humans, altering the normal microbial flora.1) The number and species of bacterial populations depends on the stage of human life: premenarche, menarche, menstrual cycle, active reproductive cycle, or menopause. There is not much knowledge about the microbiological implications of the steroid-hormonal effect on the urogenital tract. Different characteristics can be influenced by hormones involved in the maintenance of the micro ecosystem.2) The first is the vaginal epithelium that represents a system with constant cellular renovation. Cellular maturation, width of surface layer, and cell desquamation depend on the estrogenic stimulation period 1) in which the cells are divided. The second one is the microbial flora itself. The concept that the urogenital tract is colonized only by lactobacilli has been modified, particularly by those researchers who isolated anaerobic microorganisms, describing a diversity of microbial species.3,4) The third aspect to be considered is the complexity of interactions between host-microorganisms and endogenous and exogenous species which can be, in turn, antagonistic or synergistic. 1)In a previous paper, an experimental model to study the protective and preventive effect of lactobacilli in the urogenital tract of mice was set up, 5) and the antibiotic effect on the colonization kinetics of both L. fementum and uropathogenic E. coli was studied. 6) L. fermentum was intraurethrally inoculated as agarose beads, and E. coli as a suspension. The aim of the present work was to study the effect of estradiol on the kinetics of colonization of both groups of microorganisms, and the histological modifications produced by them. MATERIALS AND METHODSMicroorganisms L. fermentum CRL 1058 was isolated from the vagina of BALB/c mice, as described before.7) E. coli was isolated from the infected urinary tract of adult women and identified by biochemical tests according to Orskov.8) The latter strain was uropathogenic, because it possessed type P fimbriae and produced hemolysins, and also showed pyelonephritogenic effects when subjected to a hemagglutination test, as tested in mice. 9)Culture Media and Growth Conditions Media, lactobacilli culture conditions, and the preparation of microscopic agarose beads for intra-urethral administration were described previously.10) Uropathogenic strains were grown in either Brain Heart Infusion (BHI) supplemented with 0.5% yeast extract, or LAPTg broth.11) Not more than three-fold subcultures were performed in order to avoid changes in adhesion properties. Microorganisms were harvested by centrifugation, washed twice with phosphate buffer saline (PBS) pH 7.0 and suspended in 0.5% peptone-water. For intra-urethral inoculation of pathogens, suspensions of microorganisms were prepared in 0.5% peptone-water. Microorganism concentrations used are indicated in each experiment.Animals Two-month-old female BALB/c mice from the inbreed colony of the Instituto de Microbio...

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Pathogenesis of urinary tract infections — Amoxicillin induces genital escherichia coli colonization

Cited by 27 publications

References 21 publications

Spontaneous Clearance of Asymptomatic Bacteriuria in Infants

Spontaneous Clearance of Asymptomatic Bacteriuria in Infants

Induction of protective immunity after escherichia coli bladder infection in primates. Dependence of the globoside-specific P-fimbrial tip adhesin and its cognate receptor.

Experimental Administration of Estradiol on the Colonization of Lactobacillus fermentum and Escherichia coli in the Urogenital Tract of Mice.

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