Pathogenesis-related gene expression in rice is correlated with developmentally controlled Xa21-mediated resistance against Xanthomonas oryzae pv. oryzae

Ponciano, Grisel; Yoshikawa, Masayasu; Lee, Jamie L.; Ronald, Pamela C.; Whalen, Maureen C.

doi:10.1016/j.pmpp.2007.03.002

Cited by 34 publications

(23 citation statements)

References 44 publications

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“…Moreover, high levels of transcripts are noted only during maximum tillering and panicle initiation stages implying that it is likely developmentally controlled. The same transcriptional characteristic has been reported for the model Xa21-Xoo pathosystem wherein Xa21-mediated resistance to Xoo is not fully expressed in early stages of development but showed increasing magnitude of resistance as the plant matures (Ponciano et al 2007). Expression of OsCYP71 peaked at 4 to 8 hours after infection of Xoo which indicates crucial involvement during early response.…”

Section: Discussionsupporting

confidence: 74%

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Overexpression of rice premnaspirodiene oxygenase reduces the infection rate ofXanthomonas oryzaepv.oryzae

et al. 2016

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract Plants utilize cytochrome P450, a large superfamily of heme-containing mono-oxygenases, in the synthesis of lignins, UV protectants, pigments, defense compounds, fatty acids, hormones, and signaling molecules. Despite the overwhelming assortment of rice P450 accession numbers in the database, their functional studies are lacking. So far, there is no evidence involving rice P450 in disease immunity. Most of our understanding has been based on other plant systems that are mostly dicot. In this study, we isolated the cytochrome P450 (OsCYP71) in rice, and screened the gene using gain-of-function technique. The full-length cDNA of OsCYP71 was constitutively overexpressed using the 35S promoter. We then explored the functions of OsCYP71 in the rice -Xanthomonas oryzae pv. oryzae pathosystem. Using the gene expression assays, we demonstrate the interesting correlation of PR gene activation and the magnitude of resistance in P450-mediated immunity.

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Section: Discussionsupporting

confidence: 74%

“…PR gene expression in rice is correlated with developmentally controlled Xa21-mediatd resistance against Xoo (Ponciano et al 2007). It was also reported to respond against Magnaporthe grisea (Jwa et al 2001).…”

Section: Introductionmentioning

confidence: 98%

Overexpression of rice premnaspirodiene oxygenase reduces the infection rate ofXanthomonas oryzaepv.oryzae

et al. 2016

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“…PR1 is a defense-related marker gene which is known to be induced during the resistant response in Arabidopsis, rice, and other plant species (Kinkema et al 2000;Liu et al 2007). We have previously reported that OsPR1 transcripts significantly accumulate following the XA21-mediated immune response (Park et al 2010c;Ponciano et al 2007). RT-PCR was performed with primers targeting Myc-Xa21 and OsPR1a (Fig.…”

Section: Establishment Of a Synchronized Xa21-mediated Immune Responsementioning

confidence: 99%

Establishment of Glucocorticoid-Mediated Transcriptional Induction of the Rice XA21 Pattern Recognition Receptor

2011

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The rice pattern recognition receptor, XA21, confers robust resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo). We have generated transgenic plants that express Xa21 only in the presence of the glucocorticoid hormone, dexamethasone (DEX). DEX-mediated transcriptional induction of Xa21 is accompanied by upregulation of pathogenesis-related 1 gene expression and restriction of Xoo multiplication. The DEXinducible system can be used to synchronize the XA21-mediated response to infection throughout the entire leaf, facilitating the study of innate immunity.

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“…oryzae (Xoo) Mitsuhara et al 2008;Ponciano et al 2007;Chittoor et al 1997), and rice dwarf virus (Shimizu et al 2007), environmental stresses (salt, wounding, cold, and drought) (Kim et al 2008;Hashimoto et al 2004), and chemical treatments (probenazole, jasmonic acid, salicylic acid, and H 2 O 2 ) (Kim et al 2008;Mahmood et al 2007;Nakashita et al 2001). Oschib1 encodes a class III chitinase and a high level of its mRNA was detected after inoculation with M. grisea or Xoo (Park et al 2004).…”

Section: Introductionmentioning

confidence: 99%

“…The transcription of RPR10a was induced within 12 h after inoculation with M. grisea (McGee et al 2001). In the Xa21-mediated resistance response to Xoo, the transcriptions of OsPR1a, OsPR1b, and OsPR10a had increased by 3 days after inoculation (Ponciano et al 2007). In transgenic rice plants, the activation of PR proteins in the absence of induction by chemical or pathogen treatment were demonstrated in the over-expression of a rice thaumatin-like protein (PR-5) (Datta et al 1999), NH1, a rice NPR1 homology (Chern et al 2005), OsRir1b (Schaffrath et al 2000), OsWR-KY03 (Liu et al 2005), and even PRm, a PR gene cloned from maize (Gomez-Ariza et al 2007), and in most cases, the transgenic rice plants showed enhanced resistance to pathogens.…”

Section: Introductionmentioning

confidence: 99%

Characteristic expression of rice pathogenesis-related proteins in rice leaves during interactions with Xanthomonas oryzae pv. oryzae

Hou

Bai

et al. 2011

Plant Cell Rep

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Pathogenesis-related (PR) proteins play an important role in the disease resistance response. To better understand the function of rice PR proteins, we examined the expressions of ten PR proteins in rice leaves at different developmental stages with or without the interaction between rice and Xanthomonas oryzae pv. oryzae (Xoo). The results showed that most of the PR proteins were expressed in rice leaves in normal growth conditions, suggesting that they play a role in rice growth. Six out of ten PR proteins (PR1, PR2, PR3, PR4b, PR8, and PR-pha) showed enhanced expression in Xa21-mediated resistance responses at late stages after inoculation with Xoo. The remaining four PR proteins (PR5, PR6, PR15, and PR16) did not show changes in expression in the resistance response. The expressions of PR proteins in the resistance reaction were further compared with those in the susceptible reaction and a mock treatment. Interestingly, several of the PR proteins were expressed at the highest levels in the susceptible reaction and at the lowest levels in the mock treatment. Among the other four PR proteins, PR5 and PR16 showed changes in the abundance only in the susceptible response, while PR6 and PR15 showed no detectable difference in expression. These data provide fundamental knowledge about the expression of PR proteins in the interaction between rice and Xoo.

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Pathogenesis-related gene expression in rice is correlated with developmentally controlled Xa21-mediated resistance against Xanthomonas oryzae pv. oryzae

Cited by 34 publications

References 44 publications

Overexpression of rice premnaspirodiene oxygenase reduces the infection rate ofXanthomonas oryzaepv.oryzae

Overexpression of rice premnaspirodiene oxygenase reduces the infection rate ofXanthomonas oryzaepv.oryzae

Establishment of Glucocorticoid-Mediated Transcriptional Induction of the Rice XA21 Pattern Recognition Receptor

Characteristic expression of rice pathogenesis-related proteins in rice leaves during interactions with Xanthomonas oryzae pv. oryzae

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