Pathogenic Characterization of European Genotype Porcine Reproductive and Respiratory Syndrome Virus Recently Isolated in Mainland China

Sun, Ming; Yongying, Ma; Liu, Bohua; Lü, Huiying; Deng, Xiaoyu; Liu, Qiaorong; Qiao, Mu; Chen, Xi; Yang, Xinyan; Chen, Xizhao

doi:10.2174/1874357901711010083

Cited by 16 publications

(21 citation statements)

References 20 publications

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“…These two strains are pathogenic to piglets and can lead to classic PRRSV-specific lesions. The infectious pigs showed mild clinical signs and no pigs died, which indicated they are less virulent [ 58 , 63 ]. Interestingly, both of them belong to subgroup I—the Amervac PRRSV vaccine-related strains.…”

Section: The Epidemic Of Prrsv-1 In Chinamentioning

confidence: 99%

“…Interestingly, both of them belong to subgroup I—the Amervac PRRSV vaccine-related strains. HLJB1 and GZ11-G1 show 91.64% and 96.3% genome homology with the Amervac PRRSV virus, respectively [ 58 , 63 ]. Additionally, recombination analyses showed that HLJB1 is a recombinant from the Amervac vaccine and the BJEU06-1 isolate [ 64 ].…”

Section: The Epidemic Of Prrsv-1 In Chinamentioning

confidence: 99%

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The prevalent status and genetic diversity of porcine reproductive and respiratory syndrome virus in China: a molecular epidemiological perspective

Guo

Chen

et al. 2018

Virol J

189

148

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Porcine reproductive and respiratory syndrome virus (PRRSV) has been epidemic more than 30 years in America and 20 years in China. It is still one of the most important causative agents to the worldwide swine industry. Here, we systematically analyzed the prevalence status of PRRSV in China by a molecular epidemiological perspective. Now both PRRSV-1 and PRRSV-2 are circulating and approximately more than 80% of pig farms are seropositive for PRRSV. For PRRSV-2, there are four lineages (lineage 1, lineage 3, lineage 5, lineage 8) circulating in the fields. Lineage 8 (CH-1a-like) and lineage 5 (BJ-4-like) appeared almost at the same time during 1995-1996. Notably, BJ-4 shares 99.6% and 99.8% identity with VR2332 and RespPRRS MLV, respectively. It means that lineage 5 is likely to be imported from America. Now highly pathogenic PRRSV (HP-PRRSV) which was considered to be evolved from local diversity of lineage 8 strains is predominant with different variants. Lineage 3 appeared in 2010 which is mainly sporadic in south of China. Lineage 1, also known as NADC30-like strains in China, has been prevalent since 2013 and leads to PRRS pandemic again. For PRRSV-1, although sporadic at present, more than 9 provinces/regions have been reported. All the circulating strains belong to subtype I. It should be paid more attention since there are no vaccines available. Our analysis would help to deeply understand the prevalent status of PRRSV in China and provide useful information for prevention and control of porcine reproductive and respiratory syndrome (PRRS).

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Section: The Epidemic Of Prrsv-1 In Chinamentioning

confidence: 99%

Section: The Epidemic Of Prrsv-1 In Chinamentioning

confidence: 99%

The prevalent status and genetic diversity of porcine reproductive and respiratory syndrome virus in China: a molecular epidemiological perspective

Guo

Chen

et al. 2018

Virol J

189

148

View full text Add to dashboard Cite

show abstract

“…Different PRRSV isolates have distinct pathogenicity. In general, HP-PRRSV isolates have the highest virulence followed by NADC30-like and classical PRRSV2 isolates [11,17,19,41], while current Chinese PRRSV1 isolates are low virulent [8,42]. Retrospective study showed that our XJ17-5 and JSTZ1712-12 isolates were obtained from clinically diseased and healthy pigs, respectively.…”

Section: Discussionmentioning

confidence: 98%

Identification of Two Porcine Reproductive and Respiratory Syndrome Virus Variants Sharing High Genomic Homology but with Distinct Virulence

Chen

Huang

et al. 2019

Viruses

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Porcine reproductive and respiratory syndrome virus (PRRSV) causes huge economic loss to the global swine industry. Even though several control strategies have been applied, PRRS is still not effectively controlled due to the continuous emergence of new variants and limited cross-protection by current vaccines. During the routine epidemiological investigation in 2017, two PRRSV variants were identified from a severe abortion farm and a clinically healthy farm, respectively. The viruses were isolated and denominated as XJ17-5 and JSTZ1712-12. Genomic sequencing indicated that their genomes are both 14,960 bp in length sharing 99.45% nucleotide identity. Sequence alignments identified a discontinuous 30-amino-acid deletion and a continuous 120-amino-acid deletion in nsp2 of both isolates. Genome-based phylogenetic analysis confirmed that XJ17-5 and JSTZ1712-12 belong to the HP-PRRSV subtype but form a new branch with other isolates containing the same 150-amino-acid deletion in nsp2. Pathogenic analysis showed that XJ17-5 is highly virulent causing 60% mortality, while JSTZ1712-12 is avirulent for piglets. Furthermore, fragment comparisons identified 34-amino-acid differences between XJ17-5 and JSTZ1712-12 that might be associated with the distinct virulence. The identification of highly homologous HP-PRRSV variants with new genetic feature and distinct virulence contributes to further analyze the pathogenesis and evolution of PRRSV in the field.

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“…However, compared with the large number of PRRSV-2 infections in China, the number of PRRSV-1 infections detected was relatively low (Lin et al, 2020;Zhai et al, 2018). We speculated that the reason might be that PRRSV-1 is not given much attention because it is not the main epidemic strain in China, and there is evidence that most PRRSV-1-infected pigs in China exhibit mild clinical symptoms (Ming et al, 2017;X. Wang et al, 2016).…”

Section: Introductionmentioning

confidence: 98%

Emergence of a novel PRRSV-1 strain in mainland China: a recombinant strain derived from the two commercial modified live viruses Amervac and DV

Sun

et al. 2022

Preprint

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Porcine reproductive and respiratory syndrome virus 1 (PRRSV-1) is one of the main pathogens causing porcine reproductive and respiratory syndrome (PRRS). In recent years, the detection number of PRRSV-1 in China has gradually increased, and the PRRSV-1 strains reported in China belong to subtype Ⅰ (Global; Clade A-L). In the present study, a novel PRRSV-1 strain, TZJ2134, was found during epidemiological surveillance of PRRSV-1 in Shandong Province in China. We obtained two fragments of TZJ2134: TZJ2134-L12 (located at nt 1672-nt 2112 in the partial Nsp2 gene) and TZJ2134-(A+B) (located at nt 7463-nt 11272 in the partial Nsp9, complete Nsp10 and partial Nsp11 genes). Phylogenetic and recombination analyses based on the two sequences showed that TZJ2134 is a recombinant strain derived from two commercial PRRSV-1 modified live vaccine (MLV) strains (the Amervac vaccine and DV vaccine strains), forming a new recombinant subgroup of DV+Amervac-like isolates with other strains. However, PRRSV-1 MLV is not currently allowed for use in China. We speculated that TZJ2134 might have been introduced from Europe. This study is the first to provide information on two recombinant PRRSV-1 MLV strains in China and provides new data for the epidemiological study of PRRSV-1 in China.

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Pathogenic Characterization of European Genotype Porcine Reproductive and Respiratory Syndrome Virus Recently Isolated in Mainland China

Abstract: Abstract:Background:

Cited by 16 publications

References 20 publications

The prevalent status and genetic diversity of porcine reproductive and respiratory syndrome virus in China: a molecular epidemiological perspective

The prevalent status and genetic diversity of porcine reproductive and respiratory syndrome virus in China: a molecular epidemiological perspective

Identification of Two Porcine Reproductive and Respiratory Syndrome Virus Variants Sharing High Genomic Homology but with Distinct Virulence

Emergence of a novel PRRSV-1 strain in mainland China: a recombinant strain derived from the two commercial modified live viruses Amervac and DV

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