Pathogenicity and Virulence of Purpureocillium lilacinum (Hypocreales: Ophiocordycipitaceae) on Mexican Fruit Fly Adults

Toledo-Hernández, Ricardo Alberto; Toledo, Jorge; Valle, Javier; Holguín-Meléndez, Francisco; Liedo, Pablo; Huerta‐Palacios, Graciela

doi:10.1653/024.102.0204

Cited by 23 publications

(14 citation statements)

References 43 publications

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“…The failure of baculovirus and protozoa to cause disease against olive fruit flies [ 24 ] has prompted researchers to focus on entomopathogenic nematodes [ 17 ] and entomopathogenic fungi (EPF). A number of studies have evaluated EPF against Mediterranean fruit fly Ceratitis capitata (Wiedemann) [ 25 , 26 , 27 ], Mexican fruit fly Anastrepha ludens (Loew) [ 28 , 29 , 30 ], European cherry fruit fly, Rhagoletis cerasi (L.) [ 31 ], western cherry fruit fly R. indifferens Curran [ 32 ], B. oleae (Rossi) [ 33 ], and R. pomonella (Walsh) [ 18 ]. However, very little work utilizing EPF isolates has been conducted on B. zonata [ 34 , 35 , 36 ] B.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Locally Isolated Entomopathogenic Fungi against Multiple Life Stages of Bactrocera zonata and Bactrocera dorsalis (Diptera: Tephritidae): Laboratory and Field Study

et al. 2021

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Fruit flies including Bactrocera zonata and B. dorsalis (Diptera: Tephritidae) are considered major pests of orchard systems in Pakistan. This study evaluated the laboratory virulence, sub-lethal effects, horizontal transmission, greenhouse, and field-cage efficacy of locally isolated entomopathogenic fungi (EPF) against B. zonata and B. dorsalis. In virulence assays against third instars and adults, all 21 EPF isolates (Beauveria bassiana and Metarhizium anisopliae) tested were pathogenic and caused varying levels of mortality to the fruit flies. Based on the initial screening, four isolates (B. bassiana WG-21 and WG-18 and M. anisopliae WG-07 and WG-02) were selected for further study. The isolate WG-18 was the most virulent against larvae and adults of B. zonata and B. dorsalis followed by WG-21, WG-02, and WG-07. In both species, adults were more susceptible than larvae to all isolates, and pupae were the least susceptible. Isolates WG-18 and WG-21 strongly decreased female fecundity and fertility, the highest adult and larval mortality, and longest developmental time of larvae and pupae. Fungal conidia were disseminated passively from infected to healthy adults and induced significant mortality, particularly from infected males to non-infected females. In greenhouse and field-cage experiments, WG-18 and WG-21 were the most effective isolates in reducing adult emergence when applied to larvae and pupae of both fruit fly species. Our results indicate that B. bassiana isolates WG-18 and WG-21 were the most virulent against multiple life stages of B. zonata and B. dorsalis, and also exerted the strongest sub-lethal effects.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Locally Isolated Entomopathogenic Fungi against Multiple Life Stages of Bactrocera zonata and Bactrocera dorsalis (Diptera: Tephritidae): Laboratory and Field Study

et al. 2021

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“…The appressoria structure and the enzymes secreted by fungi are considered as the crucial virulence factors. P. lilacinum had also been known to be both nematophagous and entomopathogenic [ 37 , 38 , 39 ]. The results from our previous [ 21 ] and this study proved that P. taiwanensis and Samsoniella sp.…”

Section: Discussionmentioning

confidence: 99%

The Evaluation of Egg-Parasitic Fungi Paraboeremia taiwanensis and Samsoniella sp. for the Biological Control of Meloidogyne enterolobii on Chinese Cabbage

et al. 2020

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Meloidogyne enterolobii, an aggressive plant-parasitic nematode, has been causing great yield loss worldwide in recent years. With no resistant Chinese cabbage cultivar available currently, a biological control strategy is needed to offer an eco-friendly option for sustainable farming. In this study, the nematode suppression efficacy of two newly isolated fungi, Paraboeremia taiwanensis and Samsoniella sp., were evaluated against M. enterolobii and compared to the known biological control agents Hyalorbilia oviparasitica strain DoUCR50 and Purpureocillium lilacinum strain 251 (PL251). Both P. taiwanensis and Samsoniella sp. reduced 29–63% disease severity as effectively as the commercial product PL251 on Chinese cabbage in greenhouse trails. The in vitro egg infection rate was 47.83% by P. taiwanensis and 47.50% for Samsoniella sp., respectively. A special protocol for scanning electron microscope observation of the fungi-infected nematodes was established in this study, and the egg parasitism of the four fungi against M. enterolobii was further confirmed. For all fungi examined in this study, fungal hyphae were seen apparently penetrating into M. enterolobii eggs without destructive damage of the overall outer eggshell and the hyphae continued to grow within eggs after 6 days of infection. The results of this study imply a similar egg-parasitism mechanism for P. taiwanensis, Samsoniella sp., H. oviparasitica DoUCR50, and P. lilacinum PL251. It further enlightens the application potential of nematophagous fungi as biocontrol agents against plant-parasitic nematodes in vegetable crop management.

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“…Sharda et al (2019) found that Talaromyces contains high levels of indoleacetic acid (IAA) and phosphate dissolving activity, which is very important for promoting plant growth. Hypocreales are the fungus that controls nematodes and is reported as an insect pathogen (Toledo et al 2019). As a whole, the abundance of bene cial bacteria in is higher than that of harmful bacteria.…”

Section: Discussionmentioning

confidence: 99%

Comparison of rhizosphere soil microbial diversity of different introduced alfalfa varieties

Ma¹,

Qi²,

Yu³

et al. 2020

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Alfalfa (Medicago sativa L.) is an important forage legume in farming and animal husbandry systems. In this study, MiSeq high-throughput sequencing was applied to assess the relationship between bacterial and fungal community structures and alfalfa growth characteristics and soil physical and chemical properties induced by different cultivars alfalfa (Victoria, Kangsai, Aohan) in the grey desert soil. The results showed that the diversity of bacterial and fungal in Victoria was higher, and the bacterial diversity was significantly lower for alfalfa with Aohan than for the others, and the fungal diversity was lower for alfalfa with Kangsai than for the others. Heatmap showed that total nitrogen, fresh weight, pH and organic have significantly affect fungal community structure, whereas pH and organic carbon also significant effects on bacterial community structure. LefSe analysis showed that the growth adaptability of introduced alfalfa is mainly related to fungal and bacterial species, and the beneficial microorganisms with significant differences and relative high abundance are significantly enriched in Victoria. Pathogens with high relative abundance are mainly concentrated in Aohan alfalfa soil. Based on our findings, Victoria is the high-yield alfalfa suitable for planting in gray desert soil, while planting Kangsai and Aohan alfalfa needs probiotic for adjuvant.

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Pathogenicity and Virulence of Purpureocillium lilacinum (Hypocreales: Ophiocordycipitaceae) on Mexican Fruit Fly Adults

Cited by 23 publications

References 43 publications

Evaluation of Locally Isolated Entomopathogenic Fungi against Multiple Life Stages of Bactrocera zonata and Bactrocera dorsalis (Diptera: Tephritidae): Laboratory and Field Study

Evaluation of Locally Isolated Entomopathogenic Fungi against Multiple Life Stages of Bactrocera zonata and Bactrocera dorsalis (Diptera: Tephritidae): Laboratory and Field Study

The Evaluation of Egg-Parasitic Fungi Paraboeremia taiwanensis and Samsoniella sp. for the Biological Control of Meloidogyne enterolobii on Chinese Cabbage

Comparison of rhizosphere soil microbial diversity of different introduced alfalfa varieties

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