Pathogenicity of Campylobacter jejuni for Turkeys and Chickens

Km, Lam; Aj, DaMassa; Morishita, TY; Hl, Shivaprasad; Aa, Bickford

doi:10.2307/1591513

Cited by 34 publications

(14 citation statements)

References 9 publications

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“…However, there have been some studies with C. jejuni . The embryonic mortality in C. coli contaminated eggs in this study is comparable to previously published results (12, 13), in which the inoculation of some strains of C. jejuni in the corioalantoid membrane was found to be lethal to embryos. Zaki e Reda (27) found 0.7% early mortality in chicken embryos positive for C. jejuni after inoculation; late embryonic mortality was 1.8% and 2.1% of chicks broke the shell but did not fully hatch.…”

Section: Resultssupporting

confidence: 91%

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Transmission of Campylobacter coli in chicken embryos

Rossi¹,

Fonseca²,

Melo³

et al. 2012

Braz. J. Microbiol.

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Campylobacter coli is an important species involved in human cases of enteritis, and chickens are carriers of the pathogen mainly in developing country. The current study aimed to evaluate the transmission of C. coli and its pathogenic effects in chicken embryos. Breeder hens were inoculated intra-esophageally with C. coli isolated from chickens, and their eggs and embryos were analyzed for the presence of bacteria using real-time PCR and plate culture. The viability of embryos was verified. In parallel, SPF eggs were inoculated with C. coli in the air sac; after incubation, the embryos were submitted to the same analysis as the embryos from breeder hens. In embryos and fertile eggs from breeder hens, the bacterium was only identified by molecular methods; in the SPF eggs, however, the bacterium was detected by both techniques. The results showed no relationship between embryo mortality and positivity for C. coli in the embryos from breeder hens. However, the presence of bacteria is a cause of precocious mortality for SPF embryos. This study revealed that although the vertical transmission is a possible event, the bacteria can not grow in embryonic field samples.

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Section: Resultssupporting

confidence: 91%

“…There were 17 viable embryos and negative in real-time PCR and 1 embryos precocity died, which was positive in real-time PCR. Most researchers claim that vertical transmission is not possible, but older works mention that, under experimental conditions, the bacteria caused embryonic mortality when inoculated in eggs (12, 13, 27). …”

Section: Resultsmentioning

confidence: 99%

Transmission of Campylobacter coli in chicken embryos

Rossi¹,

Fonseca²,

Melo³

et al. 2012

Braz. J. Microbiol.

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“…Campylobacter infection in poultry may be asymptomatic (45,64), although diarrhea, reduced weight gain, and mortality have been reported for infected birds (30,52,53). In our study, C. jejuni was recovered from the jejuna and ceca of infected chicks for up to 14 days postinfection, indicating that the intestinal tract was colonized.…”

Section: Discussionmentioning

confidence: 46%

Epidermal Growth Factor Inhibits Campylobacter jejuni -Induced Claudin-4 Disruption, Loss of Epithelial Barrier Function, and Escherichia coli Translocation

et al. 2008

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Campylobacter jejuni is a leading cause of acute bacterial enteritis in humans. Poultry serves as a major reservoir of C. jejuni and is thought to act as a principal vehicle of transmission to humans. Epidermal growth factor (EGF) is a small amino acid peptide that exerts a broad range of activities on the intestinal epithelium. The aims of this study were to determine the effect of EGF on C. jejuni intestinal colonization in newly hatched chicks and to characterize its effects on C. jejuni-induced intestinal epithelial barrier disruption. White Leghorn chicks were treated with EGF daily, starting 1 day prior to C. jejuni infection, and were compared to control and C. jejuni-infected, EGF-treated chicks. Infected chicks shed C. jejuni in their feces throughout the study period. C. jejuni colonized the small intestine and cecum, disseminated to extraintestinal organs, and caused jejunal villus atrophy. EGF reduced jejunal colonization and dissemination of C. jejuni to the liver and spleen. In EGF-treated C. jejuni-infected chicks, villus height was not significantly different from that in untreated C. jejuni-infected chicks or controls. In vitro, C. jejuni attached to and invaded intestinal epithelial cells, disrupted tight junctional claudin-4, and increased transepithelial permeability. C. jejuni also promoted the translocation of noninvasive Escherichia coli C25. These C. jejuni-induced epithelial abnormalities were abolished by pretreatment with EGF, and the effect was dependent upon activation of the EGF receptor. These findings highlight EGF's ability to alter colonization of C. jejuni in the intestinal tract and to protect against pathogen-induced barrier defects.

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“…This finding suggests that sequence variation of cmp may be exploited for molecular typing or phylogenetic analysis of C. jejuni. The turkey strain (Turk), which was isolated in California in the early 1990s (23), had an identical cmp gene to that of four human isolates isolated in Ohio (Fig. 4).…”

Section: Discussionmentioning

confidence: 96%

Sequence Polymorphism, Predicted Secondary Structures, and Surface-Exposed Conformational Epitopes of Campylobacter Major Outer Membrane Protein

et al. 2000

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The major outer membrane protein (MOMP), a putative porin and a multifunction surface protein of Campylobacter jejuni, may play an important role in the adaptation of the organism to various host environments. To begin to dissect the biological functions and antigenic features of this protein, the gene (designated cmp) encoding MOMP was identified and characterized from 22 strains of C. jejuni and one strain of C. coli. It was shown that the single-copy cmp locus encoded a protein with characteristics of bacterial outer membrane proteins. Prediction from deduced amino acid sequences suggested that each MOMP subunit consisted of 18 ␤-strands connected by short periplasmic turns and long irregular external loops. Alignment of the amino acid sequences of MOMP from different strains indicated that there were seven localized variable regions dispersed among highly conserved sequences. The variable regions were located in the putative external loop structures, while the predicted ␤-strands were formed by conserved sequences. The sequence homology of cmp appeared to reflect the phylogenetic proximity of C. jejuni strains, since strains with identical cmp sequences had indistinguishable or closely related macrorestriction fragment patterns. Using recombinant MOMP and antibodies recognizing linear or conformational epitopes of the protein, it was demonstrated that the surface-exposed epitopes of MOMP were predominantly conformational in nature. These findings are instrumental in the design of MOMP-based diagnostic tools and vaccines.

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Pathogenicity of Campylobacter jejuni for Turkeys and Chickens

Cited by 34 publications

References 9 publications

Transmission of Campylobacter coli in chicken embryos

Transmission of Campylobacter coli in chicken embryos

Epidermal Growth Factor Inhibits Campylobacter jejuni -Induced Claudin-4 Disruption, Loss of Epithelial Barrier Function, and Escherichia coli Translocation

Sequence Polymorphism, Predicted Secondary Structures, and Surface-Exposed Conformational Epitopes of Campylobacter Major Outer Membrane Protein

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