Pathogenicity of Czech Isolates of Infectious Bursal Disease Virus

Juranová, R.; Nga, Nguyen Thi Ngoc; Kulikova, L. N.; Jurajda, V.

doi:10.2754/avb200170040425

Cited by 9 publications

(7 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These observations are in accordance with those reported by Juranova et al . [ 22 ] and Khan et al . [ 23 ], while in the sixth farm most of the necropsied birds were found to have an enlarged and hemorrhagic bursa ( Figure-1 ).…”

Section: Resultsmentioning

confidence: 99%

Histopathological and immunohistochemical diagnosis of infectious bursal disease in poultry birds

Singh¹,

Banga²,

Brar³

et al. 2015

Vet World

View full text Add to dashboard Cite

Aim:The aim of the present study was to diagnose infectious bursal disease (IBD) using gross, histopathological, and immunopathological approaches and to compare efficacy of immunohistochemical techniques with conventional diagnostic techniques. Materials and Methods: A total of 33 samples were collected from the six different poultry farms from Ludhiana and the nearby districts. Upon gross analysis of the necropsied birds, the relevant tissue samples such as bursa, kidney, junction of proventriculus and gizzard, heart, and muscles were then processed for histopathological and immunohistochemical studies. Results: Varied macroscopic changes were noted in bursa, characterized as swollen, hemorrhages to atrophy in size. Nonetheless, hemorrhages over thigh muscles were rarely seen. Histologically, the bursa showed prominent fibrotic and atrophic changes. Rarefaction of bursal follicles with intermittent infiltration of lympho-mononuclear cells with chronic cystic changes was additional changes, considered to be paramount for IBD. Expression and localization of IBD specific viral antigens were noticed mainly intracellular to the rarefied areas of bursal follicle section(s), in conjunction to inner lining of the cystic cavities of affected follicles. In addition, the junction of proventriculus and gizzard, the heart muscle, respiratory ciliated epithelium, and proventriculus also revealed positive expression to IBD virus (IBDV) antigen. Advanced immunopathological techniques, i.e., immunofluorescence further testified the evidence of antigen as positive green signal within affected follicles. Further consideration to the reliability of various techniques employed, positive correlation (r=0.64623) was emerged out with conventional pathological scoring. Conclusion: It is concluded that the bursa acts as an organ of choice for demonstrating IBDV antigen for specific diagnosis of disease using immunohistochemistry (IHC), and IHC staining is a precise, specific, rapid, and reliable method to demonstrate the IBDV antigen in the altered tissues due to IBDV infection.

show abstract

Section: Resultsmentioning

confidence: 99%

Histopathological and immunohistochemical diagnosis of infectious bursal disease in poultry birds

Singh¹,

Banga²,

Brar³

et al. 2015

Vet World

View full text Add to dashboard Cite

show abstract

“…Acute bursal diseases cases were mainly reported from commercial broiler chicken feeding farms and laying broiler pullet farms, and were rare in non-commercial flocks (Juranová et al 2001).The basis for infectious bursal disease prevention is specific immunoprophylaxis. Inactivated oil vaccines and live apathogenic or attenuated vaccines of various virulence levels are used.…”

mentioning

confidence: 99%

Effects of Infectious Bursal Disease Vaccination Strains on the Immune System in Leghorn Chickens

Kulikova¹,

Jurajda²,

Juranová³

2004

Acta Vet. Brno

Self Cite

View full text Add to dashboard Cite

“…This reduction in haematological parameter may be due to diseases which were confirmed in the histological indices of the broilers chicken and may not have link to the feed. Available information indicates that haematological values of avian species are significantly influenced by poultry diseases including fowl typhoid [21], mycoplasmosis [22], avian coccidiosis [23], infectious bursal disease [24,25], Newcastle disease [26] and toxoplasmosis [27]. Mohammad and Oloyede [28] reported significant reductions in the haematological contents of the blood of broiler chicks fed A. niger-fermented T. catappa seed meal-based diet [28][29][30][31].…”

Section: Discussionmentioning

confidence: 99%

Rhizopus Oryzae May Reduce Toxic Effects of Raw Mango Seed Kernel Amendment in the Diet of Broilers

Ibrahim¹

2018

NFSIJ

View full text Add to dashboard Cite

Studies on the histological and haematological indices of broilers fed Rhizopus oryzae fermented mango (Mangnifera indica) seed kernel was conducted. Mango seed kernels were seeded with Rhizopus oryzae and allowed to ferment for ten days. Bird fed fermented mango seed kernels had a mean weight of 2.35kg before slaughtering and 2.2kg after slaughtering. The mean weight of the organs were 13.3g for lungs, 8.5g for heart, 4.1g for the intestine, 1.3g for spleen, 47.9g for liver, 5.4g for bursa and 11.2g for kidney. Histological observation revealed hemorrhages and necrosis in the lung and intestine of the birds and the bursa was inflammed. The mean haematological value was 25.5% for PCV, 2.10x10 6 M/µl for RBC and 21.65x10 3 M/µl for WBC. The full blood count revealed that the chicken had neutrophils value of 9%, lymphocytes 86%, monocytes 5% and eosinophils 1.0%. In conclusion, Rhizopus oryzae could be exploited to reduce the toxic effects of raw mango seed kernels and 50% of maize could be replaced with fermented mango seed kernel in the diet of broilers without adverse effect on growth, histological and hematological indices.

show abstract

Pathogenicity of Czech Isolates of Infectious Bursal Disease Virus

Cited by 9 publications

References 11 publications

Histopathological and immunohistochemical diagnosis of infectious bursal disease in poultry birds

Histopathological and immunohistochemical diagnosis of infectious bursal disease in poultry birds

Effects of Infectious Bursal Disease Vaccination Strains on the Immune System in Leghorn Chickens

Rhizopus Oryzae May Reduce Toxic Effects of Raw Mango Seed Kernel Amendment in the Diet of Broilers

Contact Info

Product

Resources

About