Pathogenicity of newly emerged bacterial pathogens, Pseudomonas stutzeri and P. oleovorans, in the Red Sea seabream Diplodus noct

Emam, Arafah M.; Haridy, Mohie; Ahmed, Nahla Hossam Eldin

doi:10.1016/j.ejar.2022.02.001

Cited by 7 publications

(3 citation statements)

References 22 publications

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“…Moreover, ASV 154 matched Vibrio campbellii , which is a pathogen in shrimp hatcheries [ 59 ], while ASVs 15, 1921, and 3808 had highest similarity to Enterococcus faecalis , an opportunistic fish pathogen, which is one of the causative agents of haemorrhagic septicaemic disease in fish and associated with high mortalities [ 60 – 62 ]. Furthermore, ASV 96 was similar to Pseudomonas stutzeri , which is an immerging pathogen in Red Sea seabream ( Diplodus noct ) [ 63 ], while ASV 2691 matches Shewanella sp., which is also an opportunistic pathogen in fish [ 64 ]. Therefore, our hypothesis that the larval bacterial community was dominated by potentially detrimental bacteria on 22 dph and that this could be the reason for parts of the mortality, was supported also at the ASV level.…”

Section: Discussionmentioning

confidence: 99%

Exploring bacterial community composition and immune gene expression of European eel larvae (Anguilla anguilla) in relation to first-feeding diets

Benini¹,

Politis²,

Conceição³

et al. 2023

PLoS ONE

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European eel (Anguilla anguilla) is a commercially important species for fisheries and aquaculture in Europe and the attempt to close the lifecycle in captivity is still at pioneering stage. The first feeding stage of this species is characterized by a critical period between 20 to 24 days post hatch (dph), which is associated with mortalities, indicating the point of no return. We hypothesized that this critical period might also be associated with larvae-bacterial interactions and the larval immune status. To test this, bacterial community composition and expression of immune and stress-related genes of hatchery-produced larvae were explored from the end of endogenous feeding (9 dph) until 28 dph, in response to three experimental first-feeding diets (Diet 1, Diet 2 and Diet 3). Changes in the water bacterial community composition were also followed. Results revealed that the larval stress/repair mechanism was activated during this critical period, marked by an upregulated expression of the hsp90 gene, independent of the diet fed. At the same time, a shift towards a potentially detrimental larval bacterial community was observed in all dietary groups. Here, a significant reduction in evenness of the larval bacterial community was observed, and several amplicon sequence variants belonging to potentially harmful bacterial genera were more abundant. This indicates that detrimental larvae-bacteria interactions were likely involved in the mortality observed. Beyond the critical period, the highest survival was registered for larvae fed Diet 3. Interestingly, genes encoding for pathogen recognition receptor TLR18 and complement component C1QC were upregulated in this group, potentially indicating a higher immunocompetency that facilitated a more successful handling of the harmful bacteria that dominated the bacterial community of larvae on 22 dph, ultimately leading to better survival, compared to the other two groups.

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Section: Discussionmentioning

confidence: 99%

Exploring bacterial community composition and immune gene expression of European eel larvae (Anguilla anguilla) in relation to first-feeding diets

Benini¹,

Politis²,

Conceição³

et al. 2023

PLoS ONE

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“…Most of the bacteria isolated in this study have been associated with active pathogenic infections in aquatic species. 1,2,14,19,37,49,50,56,57,60,67,88 However, bacteria discounted as probable freshwater contaminants in this study population included Acinetobacter spp., Aeromonas spp., Pasteurella spp., Pseudomonas spp., and Rahnella aquatilis . Probable spoilage-associated environmental contaminants include Alcaligenes faecalis, Bergeyella zoohelcum/Weeksella virosa, Brevundimonas diminuta/vesicularis , and Shewanella algae .…”

Section: Discussionmentioning

confidence: 91%

A retrospective study of pathologic findings in cephalopods (extant subclasses: Coleoidea and Nautiloidea) under laboratory and aquarium management

Rich

Denk²,

Sangster

et al. 2023

Vet Pathol

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This retrospective study examines pathologic findings in 593 captive cephalopods (340 octopuses, 130 cuttlefish, 33 squid, and 90 nautiluses; 22 species in total) submitted to International Zoo Veterinary Group Pathology between May 2003 and August 2022. Common octopus, European common cuttlefish, hummingbird bobtail squid, and chambered nautilus were the most numerous species from the included orders of Octopoda, Sepiida, Sepiolida, and Nautilida, respectively. Commonly identified conditions included coccidiosis and renal dicyemid mesozoan infection in octopuses, amebiasis in squid, bacterial infections in cuttlefish, and idiopathic multisystemic inflammatory disease in nautiluses. Coccidiosis was most frequent in common octopuses, giant Pacific octopuses, and California 2-spot octopuses (present in 68.4%, 46.3%, and 23.8% of these species, respectively) and was the attributed cause of death in 32.3%, 36.0%, and 60.0% of such cases, respectively. Ulcerative dermatitis (UD) was common, affecting squid, cuttlefish, and octopuses, while many nautiluses exhibited black shell disease and/or UD. Notable differences in the prevalence of UD were detected between laboratory and aquaria-housed decapodiforms; 52.2% for laboratory squid versus 20.0% in aquaria; 51.3% in laboratory cuttlefish versus 11.0% in aquaria. All octopuses and nautiluses in the study were derived from aquaria. Semelparity-associated death in Coleoidea species was identified in 22.4% of octopuses, 11.5% of cuttlefish, and 6.1% of squid. This report aims to provide an overview and reassessment of species-specific disease patterns under aquarium and laboratory management as a starting point for future developments in husbandry and disease investigation.

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“…The survival rate of Red Sea seabream was only 16.6% due to Pseudomonas infection. In addition, Pseudomonas stutzeri showed multi-antibiotic resistance to streptomycin, chloramphenicol, and ciprofloxacin (Emam et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Kidney and liver histopathology of sea bass Lates calcarifer infected with black body syndrome-associated bacteria

Nuryati,

Yanti,

Rahman

et al. 2023

Jurnal Akuakultur Indonesia

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Bacterial isolation was carried out on sea bass Lates calcarifer juvenile with black body syndrome (BBS) and isolates of Pseudomonas stutzeri L01, Vibrio harveyi, Bacillus cereus, Salinicoccus roseus, and Pseudomonas stutzeri L02 were obtained. Microscopic anatomical pathology of each bacterium needs to be done to determine its pathogenicity. This study aims to compare the degree of damage to sea bass juveniles' liver and kidney organs through histopathological observations due to bacterial infections that cause BBS. The degree of histopathological damage was determined by scoring histopathological changes in the tissue under a microscope with a magnification of 40×10 in five fields of view. The data obtained were analyzed using SPSS 24 software and further tested with Kruskal-Wallis and Post Hoc (Mann Whitney). The results showed that fish liver and kidney organs in all treatments experienced necrosis compared to the control treatment, but not significantly different between bacterial infection treatments. Histopathological observations of kidney and liver organs of sea bass juveniles infected with bacteria that cause BBS show necrosis damage that was classified as normal and mild. Keywords: bacteria, histopathology, Lates calcarifer ABSTRAK Isolasi bakteri telah dilakukan pada benih ikan kakap putih yang mengalami black body syndrome (BBS) dan diperoleh isolat bakteri Pseudomonas stutzeri L01, Vibrio harveyi, Bacillus cereus, Salinicoccus roseus, dan Pseudomonas stutzeri L02. Patologi anatomi mikroskopis dari masing-masing bakteri perlu dilakukan untuk mengevaluasi patogenitasnya. Penelitian ini bertujuan membandingkan tingkat kerusakan organ hati dan ginjal benih ikan kakap putih melalui pengamatan histopatologi akibat infeksi bakteri penyebab BBS. Derajat kerusakan histopatologi ditentukan dengan melakukan skoring perubahan histopatologi jaringan di bawah mikroskop dengan perbesaran 40×10 pada lima lapang pandang. Data dianalisis menggunakan software SPSS 24 dan diuji lanjut dengan Kruskal-Wallis dan Post Hoc (Mann Whitney). Hasil pengamatan menunjukkan bahwa organ hati dan ginjal ikan pada semua perlakuan infeksi mengalami nekrosis dibandingkan dengan perlakuan kontrol, tetapi tidak berbeda nyata antar perlakuan infeksi bakteri. Hasil pengamatan histopatologi organ ginjal dan hati benih ikan kakap putih yang diinfeksi dengan bakteri yang menyebabkan BBS menunjukkan kerusakan nekrosis yang tergolong pada derajat normal dan ringan. Kata kunci: bakteri, histopatologi, kakap putih

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Pathogenicity of newly emerged bacterial pathogens, Pseudomonas stutzeri and P. oleovorans, in the Red Sea seabream Diplodus noct

Cited by 7 publications

References 22 publications

Exploring bacterial community composition and immune gene expression of European eel larvae (Anguilla anguilla) in relation to first-feeding diets

Exploring bacterial community composition and immune gene expression of European eel larvae (Anguilla anguilla) in relation to first-feeding diets

A retrospective study of pathologic findings in cephalopods (extant subclasses: Coleoidea and Nautiloidea) under laboratory and aquarium management

Kidney and liver histopathology of sea bass Lates calcarifer infected with black body syndrome-associated bacteria

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