Pathological myopia-induced antioxidative proteins in the vitreous humor

Abstract

Background: This study aimed to investigate differentially expressed proteins in the vitreous humor (VH) of pathological myopia (PM) and normal eyes.Methods: VH samples were collected from patients undergoing surgical treatment for rhegmatogenous retinal detachment (RRD), idiopathic epiretinal membrane (ERM), myopic retinoschisis (MRS) or macular hole (MH). A label-free quantitative proteomic analysis was performed to detect the differentially expressed proteins, and expression of three differentially expressed proteins was confirmed by ELISA. Results:In PM patients (MH-PM, MRS-PM and RRD-PM), the expression of prostaglandin-H2 D-isomerase (PGDS) and glutathione peroxidase 3 (GPX3) was significantly lower than in controls

Lipid Peroxidation in Subretinal Fluid: Some Light on the Prognosis Factors

“…Along the same lines, the inhibition of certain mitochondrial pathways mediated by oxidative stress preserves photoreceptors after retinal detachment [ 39 ]. This would be particularly important in the case of highly myopic eyes due to the impaired oxidative stress situation in the eyes of these patients [ 28 , 40 , 41 ] where it has also been reported that there is a decrease in the antioxidant proteins in the vitreous humor of pathological myopia patients undergoing retinal surgical treatment (among others for rhegmatogenous retinal detachment) compared with in controls [ 42 ].…”

Omics in Myopia

“…VH seems to be the best, relatively less invasively accessible surrogate material for research on human chorioretinal omics. In a study conducted by Wei et al [10], patients subjected to PPV were classified based on the lack of PM signs (AL < 26.5 mm), presence thereof (26.5 mm < AL <29.0 mm) or occurrence of high PM (>29.0 mm), and then subdivided according to a specific pathology: MH, ERM, RRD or MRS. Expression of prostaglandin-H2 D-isomerase (PGDS) and glutathione peroxidase 3 (GPX3) in the PM groups was shown to be significantly lower than in the controls.…”

“…The evidence from in vitro studies suggests that L-PGDS can potentially prevent oxidative stress and apoptosis-related neurodegenerative diseases. In turn, GPX3 is known to catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H 2 O 2 ) to protect cells from oxidative damage [10].…”

“…Animal specimens are easier to obtain than clinical samples, and the amount of analyte is larger. While proteomic analyses of human specimens were limited to vitreous [10,32] and aqueous humor [19,26,28], other materials, such as sclera [22,23], retina [17] and cornea [12], were also included in animal studies. Both studies in humans and those involving animal models can be divided into non-targeted and targeted analyses.…”

“…Thus, the methods for protein quantification have been used increasingly nowadays, with the most popular being the label-free technique and chemical labeling. The LF method was used, among others, to analyze the vitreous and retina from both humans [10,32] and animals [15,21,27]. In turn, chemical tags found application in the analysis of the proteomic profiles of the retina, vitreous and aqueous humor.…”