Pathology and immunohistochemical evaluation of Vibrio alginolyticus infection in Macrobrachium rosenbergii

Ajadi, Abdullateef; Sabri, M. Y.; Atata, J.A.; Daodu, Oluwafemi Babatunde; Emikpe, Benjamin Obukowho

doi:10.1007/s00580-018-2881-4

Cited by 8 publications

(10 citation statements)

References 19 publications

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“…Vibrio infections have caused vibriosis that has contributed to mass mortality and severe economic losses in India, Thailand, Philippines, Japan, Ecuador, Peru, Colombia and Central America (Lightner, 1996). Under the host–pathogen interactions, earlier studies have demonstrated alterations in the host ( M. rosenbergii ) at the level of morphology, biochemical profile and physiology (Ajadi et al., 2019; Shekhar & Ponniah, 2015; Tiruvayipati & Bhassu, 2016), but fewer reports have studied the innate immune regulatory mechanisms and fitness phenotypes associated with Vibrio infections in the host. Identification and quantification of molecular mechanisms of pathogenicity and fitness traits attributed to physiology of M. rosenbergii against Vibrio species would therefore provide interesting insights to host defence mechanisms.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, 14 strains of V. harveyi have similar disease symptoms such as inflamed hepatopancreas with nuclear deformation and lipid breakdown leading to 100% mortality in post‐larval juveniles of M. rosenbergii (Siripornadulsil et al., 2013). The V. alginolyticus induced Vibriosis in M. rosenbergii included lesions in hepatopancreas such as haemocytic infiltration into the interstitial space of the tubules and loss of epithelial layer and necrosis, and enlargement of lamellar sinus of the gill (Ajadi et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome analysis of Macrobrachium rosenbergii hepatopancreas in response to Vibrio harveyi infection

Baliarsingh¹,

Chung

Sahoo

et al. 2020

Aquaculture Research

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The giant freshwater prawn, Macrobrachium rosenbergii, is mass cultivated with production exceeding 200,000 tons per year since 2002. Lately, bacterial and viral infections have resulted in mortalities significantly affecting production. Vibrio harveyi causes hepatopancreatic nuclear deformation and lipid breakdown in post‐larval juvenile prawns. To facilitate the understanding of the molecular factors underlying host defence against the pathogen, we investigated the transcriptome and differentially expressed gene (DEG) profiles of the hepatopancreas from M. rosenbergii infected with V. harveyi. A total of 1,884 and 6,809 unigenes in control (PBS‐treated) and Vibrio‐infected groups, respectively, were assembled from the transcriptome. A significant proportion of unigenes (76.56%) annotated to homologous proteins in the Protostome database. KOG analysis identified the distribution of unigenes under translation, ribosomal structure and biogenesis, while GO and KEGG analysis indicated genes involved in metabolism and genetic information processing, respectively. The DEG analysis produced a total of 1,911 aberrantly expressed sequences, with 1,805 and 106 sequences significantly down‐ or upregulated (based on log2[FC]), respectively. Among the downregulated sequences, we identified ferritin, haemolysin C, chitin synthase, zinc metalloprotease and glycerol kinase. This study has provided insights to access a greater number of traits to study bacterial pathogenicity in the host, thus contributing to the defences of giant freshwater prawn.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of Macrobrachium rosenbergii hepatopancreas in response to Vibrio harveyi infection

Baliarsingh¹,

Chung

Sahoo

et al. 2020

Aquaculture Research

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“…parahaemolyticus and V. cholerae, which both cause red discolouration of infected prawns, [98][99][100] and V. alginolyticus causing mortalities in adult prawns. 101 Larvae appear to be particularly susceptible to Vibrio spp. in hatchery settings.…”

Section: Notementioning

confidence: 99%

Diseases of the giant river prawnMacrobrachium rosenbergii: A review for a growing industry

Hooper

Debnath

Stentiford

et al. 2022

Reviews in Aquaculture

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The giant river prawn, Macrobrachium rosenbergii, is a major focus of aquaculture in tropical and sub-tropical regions around the globe. Over the last 30 years, culture of M. rosenbergii has increased exponentially as demand has risen both for domestic consumption and for international export trade. As with many aquaculture species increases in production have been accompanied by the emergence of diseases affecting yield, profit and trading potential. Disease-causing agents include pathogens infecting other crustaceans, such as Decapod Iridescent Virus (DIV1), as well as pathogens only known from M. rosenbergii such as White Tail Disease caused by Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV). Here, we review the pathogenic agents associated with the culture of M. rosenbergii since commercial culture began in earnest during the 1970s. Particular emphasis is given to pathogens first identified in other aquaculture host species, but which have subsequently been shown to infect and cause disease in M. rosenbergii. As polyculture of M. rosenbergii with other aquaculture species is common practice, including culture with other decapods, crabs and fish, increased pathogen transfer among these farmed species may occur as M. rosenbergii aquaculture increases in the future.aquaculture, emerging disease, giant river prawn, polyculture | INTRODUCTIONAquaculture is the fastest-growing farmed food sector, with the proportion of cultured to caught seafood increasing year-on-year. 1,2 Global aquaculture production for fish, crustaceans and molluscs surpassed 87 million tonnes in 2020, just under half of all world production. 2 Crustacean production by aquaculture far surpasses that by capture, with more than 11,000,000 tonnes in 2020 from aquaculture compared to 6,000,000 tonnes by capture. 2 Despite these considerable production figures, it is estimated that up to 40% of tropical shrimp aquaculture production is lost annually, 3 equating to losses of over 3 billion USD, and this is primarily due to infection with viral agents. 4 Losses on this scale threaten global food security, with most of the consumption of cultured crustaceans being outside of producing countries, making management and mitigation of disease of key importance to the future success of crustacean aquaculture. 4 Stentiford et al. (2012) outlines a holistic strategy involving both producer and consumer nations to improve husbandry and farm management, better understand pathogens and their spread (either by movement of animals or alternative hosts) and learn lessons from previous disease outbreaks. This review focuses on these points to outline the pathogenic agents in Macrobrachium rosenbergii culture, identify other hosts

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“…Due to faster growth rate, better defense against pathogens, and tolerance to wide range of temperatures, the production of this species has increased substantially with greater distributional ranges (Banu and Christianus, 2016). The post-larval prawns have been found susceptible to bacterial pathogen Vibrio harveyi and M. rosenbergii nodavirus (MrNV) due to semi-intensive and intensive farming in most developing countries (Sahul Hameed et al, 2004;Murwatonko et al, 2016;Ajadi et al, 2019). A transcriptome analysis of M. rosenbergii hematopoietic tissue (hepatopancreas) was conducted before and after V. harveyi challenge using Illumina HiSeq platform to study the differential expression of genes and screen the immunity-related transcripts (Baliarsingh et al, 2020).…”

Section: O N L I N E C O P ÿmentioning

confidence: 99%

Identification and in silico characterization of hemocyanin γ-type subunit protein in Vibrio harveyi infected freshwater prawn, Macrobrachium rosenbergii

Patnaik¹,

Baliarsingh²,

Sahoo³

et al. 2021

JEB

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Aim: Identification of full-length ORF of hemocyanin subunit-1 (Mr_HC_1) from the hepatopancreas transcriptome of freshwater prawn, Macrobrachium rosenbergii infected with Vibrio harveyi and characterization of its sequence and structure by in silico tools and softwares. Methodology: Illumina HiSeq and de novo assembled unigenes were scanned against PANM-DB to screen Mr_HC_1. FGENESH gene prediction and SMART programs were used to predict the ORF region. Subsequently, Clustal X2 and MEGA in-silico tools were used to understand the sequence relatedness and evolutionary status of Mr_HC_1. Structural prediction was performed by SWISS-MODEL and Ramachandran plot modeling programs Results: The full-length ORF was 1983 bp in length encoding a polypeptide of 661 amino acid residues. Mr_HC_1 showed a putative signal peptide of 21 amino acid residues at the N-terminus and three hemocyanin domains. Homology analysis of Mr_HC_1 amino acid sequence confirms maximum identity to M. nipponense hemocyanin subunit-1 (Mn_HC_1). Phylogenetic analysis showed that Mr_HC_1 is more closely related to the hemocyanin γ-type subunit of freshwater shrimps. Homology modeling of Mr_HC_1 showed homo-hexameric protein containing 12 copper ions. With a QMEAN score of -3.33 and model-template sequence identity of 59.15%, the predicted model of Mr_HC_1 is convincing Interpretation: This study characterizes the hemocyanin γ-type subunit protein of freshwater prawn, M. rosenbergii for future studies on host defense mechanisms.

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Pathology and immunohistochemical evaluation of Vibrio alginolyticus infection in Macrobrachium rosenbergii

Cited by 8 publications

References 19 publications

Transcriptome analysis of Macrobrachium rosenbergii hepatopancreas in response to Vibrio harveyi infection

Transcriptome analysis of Macrobrachium rosenbergii hepatopancreas in response to Vibrio harveyi infection

Diseases of the giant river prawnMacrobrachium rosenbergii: A review for a growing industry

Identification and in silico characterization of hemocyanin γ-type subunit protein in Vibrio harveyi infected freshwater prawn, Macrobrachium rosenbergii

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