Pathology of Invasive and Intraepithelial Penile Neoplasia

Cañete‐Portillo, Sofía; Sánchez, Diego F.; Cubilla, Antonio L.

doi:10.1016/j.euf.2019.06.013

Cited by 18 publications

(22 citation statements)

References 8 publications

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“…The cohort of Frankfurt penile carcinomas and carcinomas in situ was associated with HPV infection in 61% (40/66 evaluable samples; 6 Carcinoma in situ, 34 invasive carcinomas) of the cases as defined by LCD-Array technique, which is in line with other studies [ 2 , 15 ] and higher than in a recent review [ 29 ]. Basal cell carcinoma was negative for HPV infection using PCR-based techniques and via p16 staining.…”

Section: Discussionsupporting

confidence: 89%

The routine use of LCD-Array hybridisation technique for HPV subtyping in the diagnosis of penile carcinoma compared to other methods

et al. 2022

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Background Routine human papillomavirus (HPV) testing is performed in cervival cancer and is required for classification of some head and neck cancers. In penile cancer a statement on HPV association of the carcinoma is required. In most cases p16 immunohistochemistry as a surrogate marker is applied in this setting. Since differing clinical outcomes for HPV positive and HPV negative tumors are described we await HPV testing to be requested more frequently by clinicians, also in the context of HPV vaccination, where other HPV subtypes are expected to emerge. Method Therefore, a cohort of archived, formalin-fixed paraffin embedded (FFPE) penile neoplasias was stained for p16 and thereafter tested for HPV infection status via PCR based methods. Additionally to Sanger sequencing, we chose LCD-Array technique (HPV 3.5 LCD-Array Kit, Chipron; LCD-Array) for the detection of HPV in our probes expecting a less time consuming and sensitive HPV test for our probes. Results We found that LCD-Array is a sensitive and feasible method for HPV testing in routine diagnostics applicable to FFPE material in our cohort. Our cohort of penile carcinomas and carcinomas in situ was associated with HPV infection in 61% of cases. We detected no significant association between HPV infection status and histomorphological tumor characteristics as well as overall survival. Conclusions We showed usability of molecular HPV testing on a cohort of archived penile carcinomas. To the best of our knowledge, this is the first study investigating LCD-Array technique on a cohort of penile neoplasias.

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Section: Discussionsupporting

confidence: 89%

The routine use of LCD-Array hybridisation technique for HPV subtyping in the diagnosis of penile carcinoma compared to other methods

et al. 2022

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“…Our analyses also demonstrated that men with tumors located at the penis glans had a reduced risk of mortality compared to those with prepuce/penis corpus/overlapping lesions. About 70% of penile SCCs originate in the mucosal epithelium of the glans 17 and typically tumors at the glans are diagnosed at earlier stages, while corpus is diagnosed at later stages. 18 Men diagnosed with poorly differentiated tumors are associated with having advanced stage and nodal metastasis.…”

Section: Discussionmentioning

confidence: 99%

Novel Prognostic Models for Patients With Penile Carcinoma

et al. 2020

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Although penile carcinoma is a rare malignancy, there is still an unmet need to identify prognostic factors associated with poor survival. In this study, we utilized demographic and clinical information to identify the most informative variables associated with overall survival in patients with penile cancer. From a full model including all covariates found to be statistically significant in univariable analyses, we identified a parsimonious reduced model containing tumor site (penis glans: hazard ratio [HR] = 0.48; 95% CI: 0.28-0.85 and penis not otherwise specified: HR = 0.45; 95% CI: 0.25-0.84), undetermined tumor differentiation (HR = 0.48; 95% CI: 0.27-0.86), and TNM stage III/IV (HR = 2.83; 95% CI: 1.68-4.75). When all of the covariates from the full model were subjected to classification and regression tree analysis, we identified 6 novel risk groups. Of particular interest, we found marriage was associated with substantial improvement in survival among men with the same stage and disease site. Specifically, among single/widowed/divorced men with TNM stage 0-II and prepuce/penis corpus/overlapping lesions had worse survival (5-year survival = 18.2%) versus married men (5-year survival = 62.5%). Since marital status is linked to social support, these findings warrant a deeper investigation into the relationships between disease prognosis and social support in patients with penile carcinoma.

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“…Similarly, in humans, penile SCCs can be caused by high-risk HPV, even though the percentage of PV-positive tumors seems to be lower than in horses [5]. Different studies provided evidence of similarities among human and equine penile squamous cell carcinomas, suggesting that the horse could represent a good preclinical model for the human counterpart [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

PD-L1/PD-1 and CTLA-4 Expression in Equine Penile Squamous Cell Carcinomas

Porcellato

Mecocci

Brachelente

et al. 2021

Animals

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In horses, penile squamous cell carcinomas (epSCCs) are among the most common cutaneous neoplastic lesions. These tumors usually arise in benign lesions such as viral plaques and papillomas frequently induced by Equus caballus papillomavirus type 2 (EcPV2) infection. In the last decade, the introduction of immune checkpoint inhibitors (ICI) for the treatment of human cancers has demonstrated promising results. Among the most commonly targeted pathways, there is PD-1/PD-L1 and CTLA-4. The aim of this study is to investigate the expression of the PD-1/PD-L1 pathway and CTLA-4 in the tumor microenvironment of epSCCs to assess the feasibility of an immunotherapeutic approach. Twenty equine epithelial tumors were retrospectively selected and submitted to RT-qPCR for PD-1 and PD-L1 genes. After testing antibodies cross-reactivity by western blotting, immunohistochemistry for PD-L1 and CTLA-4 was performed. Results from RT-qPCR demonstrated that 3/20 cases expressed the PD-L1 gene, whereas the PD-1 gene was not detected. Immunohistochemical positivity for PD-L1 was found only in one case. CTLA-4-positive cells were observe in all cases but were few (Mdn = 4.8; IQR = 2.3–7.1 cells/HPF). In this study group, PD-1/PD-L1 and CTLA-4 do not appear to be highly expressed and therefore the use of ICI in epSCCs may not have promising rates of response.

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Pathology of Invasive and Intraepithelial Penile Neoplasia

Cited by 18 publications

References 8 publications

The routine use of LCD-Array hybridisation technique for HPV subtyping in the diagnosis of penile carcinoma compared to other methods

The routine use of LCD-Array hybridisation technique for HPV subtyping in the diagnosis of penile carcinoma compared to other methods

Novel Prognostic Models for Patients With Penile Carcinoma

PD-L1/PD-1 and CTLA-4 Expression in Equine Penile Squamous Cell Carcinomas

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