Pathology of melanocytic tumour in cattle

Bhadaniya, A.R.; Trangadia, B.J.; Prasad, Mahendra; Fefar, D. T.; Kalaria, V. A.; Vadaliya, J.V.; Modi, K.S.

doi:10.5958/0973-970x.2015.00060.7

Cited by 2 publications

(3 citation statements)

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“…Diagnosis of melanomas is made based on of their typical macroscopic and microscopic features (Brito et al, 2009). In this study, we observed that the tumoral mass was solitary, firm, hairless, ovalround shaped and quite large following the literature data (Babić et al, 2009;Bhadaniya et al, 2015;Vijayakumar et al, 2020). We found that the cross-sectional faces of melanomas are dark black in patches of brown as reported by Madheswaran et al, 2019.…”

Section: Discussionsupporting

confidence: 61%

“…Melanoma is the term used for all malignant melanocytic tumors, while melanocytoma refers to benign tumors (Madheswaran et al, 2019). Melanocytic neoplasms are common in dogs, gray horses and cats, they are rarely observed in large animals such as cattle (Bhadaniya et al, 2015;Vijayakumar et al, 2020). Melanocytomas, like in melanomas, is very rare in cattle (Madheswaran et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Immunohistochemical assessment of S100, Vimentin, PCNA, p53 and MMP-9 expressions in bovine melanomas

Karakurt

Aydin

Beytut

et al. 2021

Harran Üniversitesi Veteriner Fakültesi Dergisi

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This study aimed to evaluate the expression of PCNA, p53, MMP-9, Vimentin and S100 immunohistochemically and determine the aggressiveness in diagnosis of bovine melanomas. The material of this study consisted of melanoma biopsy samples taken from 10 cattle brought to our department. Tissue samples from cattle were fixed in 10% buffered formalin solution. After routine procedures paraffin blocks were cut sections of 5 μm thickness. For bleaching, some heavily pigmented tumour sections were deparaffinized, hydrated, and incubated in 10% solution of hydrogen peroxide (H2O2) for 5 hours at 65 o C until sections appeared clear and Hematoxylin & Eosin (H&E) staining was applied to the sections to detect histopathological changes. Sections were examined and photographed under a light microscope. Avidin-Biotin Peroxidase was used as the immunohistochemical method. We observed that the tumoral mass was solitary, firm, hairless, oval-round shaped and quite large. We detected spindle and epithelioid type tumoral cells containing a lot of large brownish-black granular melanin pigments in their cytoplasm. All melanoma cases were immune positive for S100, Vimentin, PCNA, p53 and MMP-9 expressions. In conclusion, we think that these immunohistochemical markers are quite convenient in evaluating the prognosis and diagnosis of bovine melanomas.

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Section: Discussionsupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Immunohistochemical assessment of S100, Vimentin, PCNA, p53 and MMP-9 expressions in bovine melanomas

Karakurt

Aydin

Beytut

et al. 2021

Harran Üniversitesi Veteriner Fakültesi Dergisi

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“…Whitening methods are widely used in human medicine for histopathological diagnosis of melanocytic lesions; however, these methods have not been used in most recent animal studies, even in cases with very pigmented lesions (Semin et al, 2011;Teixeira et al, 2013;Bhadaniya et al, 2015;Pandey et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Melanin bleaching with potassium permanganate for melanocytic lesions diagnosis in veterinary medicine

Moreira

Blanca²,

Gundim

et al. 2019

Arq. Bras. Med. Vet. Zootec.

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The aim of this study was to microscopically re-evaluate the melanocytic lesions diagnoses established by the Animal Pathology Laboratory of the Federal University of Uberlândia, in the state of Minas Gerais, Brazil, over a period of eleven years; in addition, to perform a comparative analysis between the conventional histopathological (CH) method and the use of the melanin bleaching (MB) technique with potassium permanganate, sulfuric acid, and oxalic acid solutions. The results of the MB method presented a disagreement in 24.32% of the diagnosis previously by CH, with low agreement (61.0%) and low Kappa coefficient (0.2267). Melanoma was the most frequent lesion, more frequent in elderly and non-breed female dogs. The most frequent melanoma location was in the cutaneous tissue. The presence or absence of a pagetoid spread in cutaneous samples, distribution of melanin, pattern of cell layout, cell morphology, degree of cellular atypia, and the number of mitoses verified after MB were the most important criteria to confirm the diagnosis of malignancy or benignity of the lesions. Evaluating pathologists considered MB to be essential for the majority of diagnoses and an efficient complementary method for the diagnosis of melanocytic lesions, even in cases with a moderate degree of pigmentation.

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Pathology of melanocytic tumour in cattle

Cited by 2 publications

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Immunohistochemical assessment of S100, Vimentin, PCNA, p53 and MMP-9 expressions in bovine melanomas

Immunohistochemical assessment of S100, Vimentin, PCNA, p53 and MMP-9 expressions in bovine melanomas

Melanin bleaching with potassium permanganate for melanocytic lesions diagnosis in veterinary medicine

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