Pathology survey of the short-neck clam Ruditapes philippinarum occurring on sandy tidal flats along the coast of Ariake Bay, Kyushu, Japan

Park, Kyung‐Il; Tsutsumi, Hiroaki; Hong, Jin Hwa; Choi, Kwang-Sik

doi:10.1016/j.jip.2008.06.004

Cited by 49 publications

(33 citation statements)

References 31 publications

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“…In wild Manila clams, the highest infection intensity of Perkinsus species is generally approximately 10 7 cells g −1 wet gill tissue (Choi et al 2002, Park et al 2008, Yoshinaga et al 2010, which is within the range that can be quantified using our method. Takahashi et al (2009) showed that the lower limit of detection by PCR-RFLP was 100 cells per 10 mg tissues (10 000 cells g −1 tissue) for P. olseni and one cell (100 cells g −1 tissue) for P. honshuensis.…”

Section: Discussionmentioning

confidence: 55%

Development of real-time PCR assays for discrimination and quantification of two Perkinsus spp. in the Manila clam Ruditapes philippinarum

Umeda

Yoshinaga

2012

Dis. Aquat. Org.

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The Manila clam Ruditapes philippinarum is infected with 2 Perkinsus species, Perkinsus olseni and P. honshuensis, in Japan. The latter was described as a new species in Mie Prefecture, Japan, in 2006. Ray's Fluid Thioglycollate Medium (RFTM) assay has been most commonly used to quantify Perkinsus infection. However, this assay cannot discriminate between species that resemble one another morphologically. We developed real-time PCR assays for the specific quantification of P. olseni and P. honshuensis. DNA was extracted using Chelex resin. Cultured P. olseni and P. honshuensis cells were counted and spiked into uninfected clam gill tissue prior to DNA extraction to generate standard curves, which allowed quantification based on the PCR cycle threshold values. We compared the RFTM assay with both real-time PCR assays by quantifying Perkinsus spp. in gill tissue samples from the same individual clams obtained from various localities in Japan. Infection intensities estimated by both assays were significantly correlated (r 2 = 0.70). Our results suggest that the prevalence and infection intensity of P. honshuensis are much lower than for P. olseni in Manila clams. KEY WORDS: Perkinsus olseni · Perkinsus honshuensis · qPCR · Ruditapes philippinarum · Manila clam · RFTM Resale or republication not permitted without written consent of the publisherDis Aquat Org 99: [215][216][217][218][219][220][221][222][223][224][225] 2012 carpet shell clam Ruditapes decussatus in Spain and Portugal (Ruano & Cachola 1986, Azevedo 1989, Goggin & Lester 1995. In East Asia, several research ers have suggested that infection with P. olseni caused mass mortalities and stock depletion of Manila clams in some re gions of Korea and Japan (Hamaguchi et al. 1998, Park & Choi 2001. Because of their potential risk to the health of aquatic mollusks, the 2 Perk insus species are listed pathogens requiring notification to the World Organization for Animal Health (OIE) (World Organization for Animal Health 2010).Until recently, it was thought that the Manila clam was infected only with Perkinsus olseni in Japan. However, Dungan & Reece (2006) described P. honshuensis, a new species, in Manila clams collected from Mie Prefecture, Japan. P. olseni is distributed throughout the tropical Pacific Ocean, Australia, the North Island of New Zealand, Vietnam, Korea, Japan, China, Portugal, Spain, France, Italy, and Uruguay. P. olseni also infects a wide range of hosts, including clams, oysters, pearl oysters, abalones, and possibly other bivalve and gastropod species (Azevedo 1989, Goggin & Lester 1995, Hamaguchi et al. 1998, Park & Choi 2001, Casas et al. 2002, Villalba et al. 2004, Cremonte et al. 2005. Perkinsus infections have been reported in Manila clams throughout Ja pan, except for regions of northern and eastern Hok kaido (Hamaguchi et al. 2002, Nishihara 2010. However, previous re ports of Perkinsus infection in Manila clams from Japan have not distinguished between the 2 species. Thus, their relative distribution is largely un known a...

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Section: Discussionmentioning

confidence: 55%

Development of real-time PCR assays for discrimination and quantification of two Perkinsus spp. in the Manila clam Ruditapes philippinarum

Umeda

Yoshinaga

2012

Dis. Aquat. Org.

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“…This specie exhibits distinct biochemical and genetic responses when exposed to heavy metals and PAHs (Ji et al, 2006;Moraga et al, 2002), possessing the requisite features of a promising indicator species. As a matter of fact, V. (R.) philippinarum has been substantiated a good bioindicator for marine environmental pollution, especially heavy metal pollution, as well as aquatic toxicology (Ji et al, 2006;Park et al, 2008).…”

Section: Modulation Of Cyp414a1 Gene Expression In Hepatopancreas Of mentioning

confidence: 99%

Identification and expression profile of a new cytochrome P450 isoform (CYP414A1) in the hepatopancreas of Venerupis (Ruditapes) philippinarum exposed to benzo[a]pyrene, cadmium and copper

Zhang

Gan

et al. 2012

Environmental Toxicology and Pharmacology

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“…Since the clam filters large amounts of seawater due to their nutritional and respiratory needs, this species of mollusc can accumulate high levels of environmental contaminants. Due to the wide distribution, long life cycle, high tolerance to salinity and temperature, ease of collection and high bioaccumulation of contaminants, the Manila clam has been considered a good bioindicator in coastal sediment pollution assessments and environmental toxicology (Park et al, 2006(Park et al, , 2008Ji et al, 2006;Liu et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Benzo(a)pyrene-induced metabolic responses in Manila clam Ruditapes philippinarum by proton nuclear magnetic resonance (1H NMR) based metabolomics

Zhang

Liu

You

et al. 2011

Environmental Toxicology and Pharmacology

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Pathology survey of the short-neck clam Ruditapes philippinarum occurring on sandy tidal flats along the coast of Ariake Bay, Kyushu, Japan

Cited by 49 publications

References 31 publications

Development of real-time PCR assays for discrimination and quantification of two Perkinsus spp. in the Manila clam Ruditapes philippinarum

Development of real-time PCR assays for discrimination and quantification of two Perkinsus spp. in the Manila clam Ruditapes philippinarum

Identification and expression profile of a new cytochrome P450 isoform (CYP414A1) in the hepatopancreas of Venerupis (Ruditapes) philippinarum exposed to benzo[a]pyrene, cadmium and copper

Benzo(a)pyrene-induced metabolic responses in Manila clam Ruditapes philippinarum by proton nuclear magnetic resonance (1H NMR) based metabolomics

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