Pathophysiologic Determination of Frostbite Under High Altitude Environment Simulation in Sprague-Dawley Rats

Hu, Jie; Li, Hua; Geng, Xiliang; Jiao, Lin; Song, Ho-Young; Lin, Liang-In; Jiao, Mingke

doi:10.1016/j.wem.2016.03.004

Cited by 12 publications

(11 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The rats in PTPF and PTPF + PEMF groups were exposed to freezing conditions for 15 seconds, and the rats in the control group were exposed to freezing conditions for 0 second. In our previous experiments, the depth and degree of PTPF were confirmed through histological observation; PTPF injury extends through the epidermis and into the dermis. After surgery was performed, the rats in the PTPF + PEMF group were exposed to low‐frequency PEMF daily at normal temperature (23 ± 2 °C).…”

Section: Methodsmentioning

confidence: 68%

See 1 more Smart Citation

Effects of low‐frequency pulsed electromagnetic fields on plateau frostbite healing in rats

Jiao

Lin²,

Jiao³

et al. 2016

Wound Repair Regeneration

Self Cite

View full text Add to dashboard Cite

Plateau frostbite (PF) treatments have remained a clinical challenge because this condition injures tissues in deep layers and affected tissues exhibit unique pathological characteristics. For instance, low-frequency pulsed electromagnetic field (PEMF) can affect tissue restoration and penetrate tissues. Therefore, the effect of PEMF on PF healing should be investigated. This study aimed to evaluate the effects of low-frequency PEMF on PF healing systematically. Ninety-six Sprague-Dawley rats were randomly and equally divided into three groups: normal control, partial thickness plateau frostbite (PTPF), and PTPF with low-frequency PEMF exposure (PTPF + PEMF). PTPF wounds were induced in the dorsum of the rats. The PTPF + PEMF group was exposed to low-frequency PEMF daily. During PF healing, wound microcirculation in each group was monitored through contrast ultrasonography. Wound appearance, histological observation, and wound tensile strength were also evaluated. Results showed that the rate of the microcirculation restoration of the PTPF + PEMF group was nearly 25% faster than that of the PTPF group, and wound appearance suggested that the healing of the PTPF group was slower than that of the PTPF + PEMF group. Histological observation revealed that PEMF accelerated the growth of different deep tissues, as confirmed by tensile strength examination. Low-frequency PEMF could penetrate PF tissues, promote their restoration, and provide a beneficial effect on PF healing. Therefore, this technique may be a potential alternative to treat PF.

show abstract

Section: Methodsmentioning

confidence: 68%

“…The operative sites of the three groups were anesthetized, aseptically prepared, and exposed to freezing conditions. Frostbite injuries with a diameter of 30 mm were induced through custom‐built liquid nitrogen penetration on the dorsum of each rat . The freezing durations for each group were varied to control the frostbite degree.…”

Section: Methodsmentioning

confidence: 99%

Effects of low‐frequency pulsed electromagnetic fields on plateau frostbite healing in rats

Jiao

Lin²,

Jiao³

et al. 2016

Wound Repair Regeneration

Self Cite

View full text Add to dashboard Cite

show abstract

“…This has limited the opportunity to study new treatments. [10][11][12][13][14][15] Recent advances in frostbite treatment involve increasing perfusion of frostbitten tissue with thrombolytic drugs such as a tissue plasminogen activator [16][17][18][19] or prostacyclin vasodilators such as iloprost. 20 , 21 Current recommendations to mitigate inflammatory injury from reperfusion have been limited to the use of nonsteroidal anti-inflammatory drugs such as ibuprofen.…”

Section: Introductionmentioning

confidence: 99%

Dexamethasone Improves Wound Healing by Decreased Inflammation and Increased Vasculogenesis in Mouse Skin Frostbite Model

Zhang

Barksdale

et al. 2020

Wilderness & Environmental Medicine

View full text Add to dashboard Cite

IntroductiondFrostbite is thought to result from initial vasoconstriction, ischemia, intracellular ice crystal formation, and inflammation caused by reperfusion injury. Corticosteroids have demonstrated beneficial anti-inflammatory effects in the treatment of other ischemia/reperfusion clinical conditions. The objective of this study was to determine the effect of dexamethasone (dex) on wound healing, inflammatory response, and vasculogenesis in a mouse skin frostbite model. MethodsdTreatment and control groups of C57/BL6 mice were subjected to frostbite using a previously described model. Treatment with intraperitoneal dex (1 mg$kg-1 $d-1) began on the day of frostbite induction and lasted for 7 d. Over 4 wk, we compared wound diameter; morphology by visual inspection, hematoxylineeosin staining, and Masson's trichrome staining; density of inflammatory cytokines IL-1b and TNFa using Western blot analysis; and formation of microvasculature using immunofluorescence staining. Data were analyzed using 1-way or 1-way repeated-measures analysis of variance. ResultsdAfter frostbite injury, morphological images demonstrated epidermal necrosis and loss in the frostbitten skin as well as infiltration of inflammation-related leukocytes. Increased production of inflammatory cytokines and disappearance of the microvasculature also occurred in the frostbitten skin. In comparison to the control group, treatment with dex promoted wound healing as demonstrated by decreased wound diameter; decreased levels of inflammatory cytokines, and accelerated formation of mature microvasculature. ConclusionsdIn this animal model, dex improved wound healing in frostbitten skin and demonstrated both anti-inflammatory effects and stimulation of vasculogenesis. This study suggests that the use of potent anti-inflammatory agents may be an effective strategy for mitigating frostbite injury.

show abstract

“…It is assessed that men in the age bracket of 30-49 are most at risk of frostbite due to greater chances of exposure. Expectedly, frostbite has been reported to affect military personnel [ 3 ] and mountaineers [ 4 ] followed by the general population [ 5 ]. Frostbite usually occurs at the exposed area where temperature is below the freezing point, i.e., -2°C (28°F).…”

Section: Introductionmentioning

confidence: 99%

“…Its severity depends upon the exposure time [ 6 ]. Frostbite injury causes loss of productivity, pain, and economic burden and has potential of permanent disability [ 3 ]. The pathogenesis of frostbite is not very clear, but according to reported studies, it includes direct cold damage to tissues and injury from ice crystal formation which causes protein disruption [ 7 ], arterial vasoconstriction, thrombus formation, and ischemia [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Cuscuta reflexa Roxb. Expedites the Healing Process in Contact Frostbite

Hassan

Buabeid

Kalsoom

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

Frostbite is caused due to extreme vulnerability to cold, resulting in damage of deeper and superficial tissues alike. In this study, we report the anti-inflammatory and wound-healing properties of aqueous methanolic extract of Cuscuta reflexa (Cs.Cr) against contact frostbite. Thirty rats were divided into five groups including three treatment groups with increasing doses of Cs.Cr, a standard drug group receiving acetylsalicylic acid (ASA), and a metal bar-induced frostbite group. Frostbite injury was induced by a 3×3.5 cm metal bar frozen up to -79°C on shaved skin for continuous 3 minutes. Wounded area percentages were recorded to measure the healing rate in response to Cs.Cr administration. Haematological parameters and malondialdehyde content were also noted. On treatment with Cs.Cr, the healing rate is drastically increased and lipid peroxidation product malondialdehyde was decreased in a dose-dependent manner. Results were compared with frostbite and ASA (standard drug group). These results indicate that Cs.Cr possesses excellent wound-healing properties against frostbite injury and can prove to be a prospective compound in such conditions.

show abstract

Pathophysiologic Determination of Frostbite Under High Altitude Environment Simulation in Sprague-Dawley Rats

Abstract: A safe and reproducible HAF model was proposed. More important, pathophysiologic determination of HAF provided the foundation and potential for developing novel and effective frostbite therapies.

Cited by 12 publications

References 38 publications

Effects of low‐frequency pulsed electromagnetic fields on plateau frostbite healing in rats

Effects of low‐frequency pulsed electromagnetic fields on plateau frostbite healing in rats

Dexamethasone Improves Wound Healing by Decreased Inflammation and Increased Vasculogenesis in Mouse Skin Frostbite Model

Cuscuta reflexa Roxb. Expedites the Healing Process in Contact Frostbite

Contact Info

Product

Resources

About