Pathotypic characterization of Newcastle disease virus isolated from vaccinated chicken in West Java, Indonesia

Putri, Dwi Desmiyeni; Handharyani, Ekowati; Soejoedono, Retno Damajanti; Setiyono, Agus; Mayasari, Ni Luh Putu Ika; Poetri, Okti Nadia

doi:10.14202/vetworld.2017.438-444

Cited by 24 publications

(23 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ND antigens which used in this study were Sato NDV and genotype VII NDV [ 11 ]. ND antigens injection were performed 3 times, and serum was collected on day 8 after third injection.…”

Section: Resultsmentioning

confidence: 99%

“…Sato and genotype VII ND antibodies were evaluated for specificity to some characterized ND isolates [ 11 ] and other antigens such as AI, IB, and IBD using AGPT. The indicator of antigen-antibody reaction at this stage was precipitation line in agarose gel.…”

Section: Resultsmentioning

confidence: 99%

“…Two NDVs were used in this study. First virus was NDV/Ck/BGR/2011 obtained from repository of the Laboratory of Immunology, Faculty of Veterinary Medicine, Bogor Agricultural University, which categorized as virulent NDV and belongs to genotype VII NDV [ 11 , 12 ]. Another virus was Sato NDV which obtained from the National Veterinary Drug and Assay Laboratory, Gunung Sindur, Bogor, Indonesia.…”

Section: Methodsmentioning

confidence: 99%

“…Agar gel precipitation test (AGPT) was used to determine the specificity of ND antibody. Specificity of ND antibody was evaluated to some characterized ND isolates [ 11 ] and other antigens such as avian influenza (AI), infectious bronchitis (IB), and infectious bursal disease (IBD). The antigen and antibody reaction was showed as precipitation line in agarose gel.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Production and characterization of Newcastle disease antibody as a reagent to develop a rapid immunodiagnostic test tool

Putri¹,

Handharyani

Soejoedono

et al. 2018

Vet World

Self Cite

View full text Add to dashboard Cite

Aim:This research was conducted to produce and characterize ND antibody as reagent candidate to develop a rapid immunodiagnostic test tool.Materials and Methods:Four New Zealand White rabbits were used in this study and divided into two groups. First group was injected by Sato ND antigen, and second group was injected by genotype VII ND antigen. This study is divided into three steps: (a) ND antibody production, (b) ND antibody purification, and (c) ND antibody characterization. First group was rabbit injected by Sato NDV (5×108.25 egg lethal doses (ELD)50/ml) and second group was injected by genotype VII NDV (5×106.5 ELD50/ml). Antigen induction was performed by subcutaneous administrated for first (day 1) and second (day 14) injection and intravenous administrated for third (day 30) injection. Blood was collected on day 8 after third injection.Results:Antibody production increased on second antigen injection and reached a peak on day 9 after second antigen injection. Sato and genotype VII ND antibody can be produced without adjuvant within 38 days with the highest titer 210. Based on antibody titer data, both antigens induced antibody production in a similar trend. The characterization antibody by SDS-PAGE indicated that molecular weight of immunoglobulin G (IgG) is 154.93 kDa (whole IgG), heavy chain 54.39 kDa, and light chain 27.74 kDa. ND antibodies have specificity to homologous and heterologous NDVs in varying virulence.Conclusion:Sato and genotype VII ND antibodies have been successfully produced within 38 days without adjuvant. Specificity of ND antibodies to NDVs in varying virulence and cross-reaction between Sato ND antibody and genotype VII ND antibody indicates that the characterized ND antibodies can be used as a reagent to develop rapid immunodiagnostic test tools.

show abstract

“…The ND antigens which used in this study were Sato NDV and genotype VII NDV [ 11 ]. ND antigens injection were performed 3 times, and serum was collected on day 8 after third injection.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Production and characterization of Newcastle disease antibody as a reagent to develop a rapid immunodiagnostic test tool

Putri¹,

Handharyani

Soejoedono

et al. 2018

Vet World

Self Cite

View full text Add to dashboard Cite

show abstract

“…Four pathotypecharacterized isolates used in this research were obtained from the repository of the Immunology Laboratory, Faculty of Veterinary Medicine, Bogor Agricultural University. Two isolates belong to virulent NDV strain (NDV/Ck/BGR/11; NDV/Ck/GS/14) and the other two belong to avirulent NDV strain (NDV/Ck//CJR/15; NDV/ Ck/BGR/15) (Putri et al, 2017).…”

Section: Newcastle Disease Virus Isolatesmentioning

confidence: 99%

Genotype Characterization of Newcastle Disease Virus Isolated from Commercial Chicken Flocks in West Java, Indonesia

Putri¹

2018

PVJ

Self Cite

View full text Add to dashboard Cite

Phylogenetic analysis of virulent strains of the Newcastle disease virus isolated from deceased chickens in Tanzania's Morogoro and Iringa regions

Amoia,

Hakizimana,

Chengula

et al. 2024

Discov Anim

View full text Add to dashboard Cite

Background Newcastle disease (ND) is a viral disease affecting a wide range of bird species and has a considerable financial impact on the world's poultry market. The ND virus (NDV) strains currently circulating in poultry throughout Africa, and especially in East Africa, exhibit significant genetic variation. Objectives The primary objective of the present investigation was to investigate the NDV genotypes in chickens raised in backyards in Tanzania's Morogoro and Iringa districts, which were associated with ND outbreaks. Methods Two tissue samples from chickens taken during a suspected ND outbreak in Tanzania's Morogoro (Eastern zone) and Iringa (Southern highlands zone) were subjected to reverse transcription polymerase chain reaction targeting the fusion (F) and hemagglutinin-neuraminidase (HN) genes, followed by sequencing. Results Based on comprehensive analysis of the entire F and HN gene sequences, the viruses were categorized as genotype VII and displayed significant genetic similarity with NDV strains previously identified in Mozambique, South Africa, Zambia, Zimbabwe, Botswana, Southeast Asia, and China. The uniformity in the amino acid cleavage site motif of the F protein across the examined NDV isolates, characterized by 112R–R–Q/K–K–R–F117, indicates their classification as virulent strains. Conclusion Regularly characterizing circulating strains and expanding the study to other parts of Tanzania may help to enhance disease control by giving a more precise picture of the situation regarding ND, especially in light of the issues posed by NDV genotype VII elsewhere.

show abstract

Pathotypic characterization of Newcastle disease virus isolated from vaccinated chicken in West Java, Indonesia

Cited by 24 publications

References 29 publications

Production and characterization of Newcastle disease antibody as a reagent to develop a rapid immunodiagnostic test tool

Production and characterization of Newcastle disease antibody as a reagent to develop a rapid immunodiagnostic test tool

Genotype Characterization of Newcastle Disease Virus Isolated from Commercial Chicken Flocks in West Java, Indonesia

Phylogenetic analysis of virulent strains of the Newcastle disease virus isolated from deceased chickens in Tanzania's Morogoro and Iringa regions

Contact Info

Product

Resources

About