Production and characterization of Newcastle disease antibody as a reagent to develop a rapid immunodiagnostic test tool

Putri, Dwi Desmiyeni; Handharyani, Ekowati; Soejoedono, Retno Damajanti; Setiyono, Agus; Poetri, Okti Nadia

doi:10.14202/vetworld.2018.895-901

Cited by 7 publications

(9 citation statements)

References 32 publications

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“…Samiullah et al [ 28 ] produced an antibody for APMV-1 using adjuvant within 91 days and reach a 1024 (2 10 ) HI titer with 4 and 5 injections. Putri et al [ 29 ] produced antibodies to Newcastle disease in New Zealand rabbits with subcutaneous route application for the first and second injection, which resulted in the same pattern of antibody titer until the 16th day after the second injection. Moreover, after the third injection intravenously, the study revealed a higher antibody titer on the 8th day, reaching HI titer of 2 9 .…”

Section: Resultsmentioning

confidence: 99%

Production of hyperimmune serum against genotype VII Newcastle disease virus in rabbits with several applications

Putri¹,

Poetri²,

Candra³

et al. 2022

J Adv Vet Anim Res

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Objective: This study aimed to produce hyperimmune serum against genotype VII Newcastle disease virus (NDV) with several applications. Materials and Methods: Production of hyperimmune serum against genotype VII NDV was performed on eight New Zealand white rabbits divided into four groups. Rabbits were immunized three times on the 1st day, the 14th day, and the 30th day. Blood sampling was carried out on the 8th day after the third immunization. Results: All groups showed the same pattern of hemagglutination inhibition (HI) titer results. HI titers would peak on the 5th or the 9th day after the second immunization, then decrease until the 3rd day after the third immunization, and increase again on the 5th day after the third immu¬nization. Rabbits immunized intravenously showed higher HI titers than the other groups. These results indicate that the intravenous route for hyperimmune serum production against genotype VII Newcastle disease virus greatly affects the immune response result. Conclusions: The production of hyperimmune serum by intravenous immunization three times was able to produce the highest titer of 210 at 38 days. The agar gel precipitation test and the Western blot assay showed that the hyperimmune serum was specific for the Newcastle disease antigen.

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Section: Resultsmentioning

confidence: 99%

Production of hyperimmune serum against genotype VII Newcastle disease virus in rabbits with several applications

Putri¹,

Poetri²,

Candra³

et al. 2022

J Adv Vet Anim Res

View full text Add to dashboard Cite

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“…According to Putri et al . [20], antibody production in laboratory animals has become an essential part of research projects. To produce antibodies, investigators are confronted with a lot of complex choices, some of which may be critical for success.…”

Section: Resultsmentioning

confidence: 99%

“…Polyclonal antibodies have a complex mixture of antibodies with different specificity, affinity, and isotype. Polyclonal antibodies have the function for binding various epitopes on the surface of the inducing antigen molecule [20]. AGPT result showed the appearance of a precipitation line near the well-containing antibody which is mean weakly positive, or it is likely that the antibody titer is low [24].…”

Section: Resultsmentioning

confidence: 99%

Sumateran wild boar (Sus scrofa vittatus) meat antibody production as immunodiagnostic reagent candidate

Adiningsih

Soejoedono

Adji³

et al. 2019

Vet World

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Aim: Meat authentication gives significance values in view of religious, food safety, public health, quality assurance, and legal concern. Most of the meat authentication is based on molecular assay; a simpler method to authenticate meat is needed to develop. An immunoassays technique may offer a solution for simpler test. The aim of our current study was to develop a polyclonal antibody of Sus scrofa vittatus (Sumateran wild boar) as an immunodiagnostic reagent candidate. Materials and Methods: Three male New Zealand white rabbits were used in this study for antibody production. Antigen used was meat extract of Sumateran wild boar, each rabbit was immunized with meat extract antigen (0.5 mg/ml) emulsified in Freund's complete adjuvant at a 1:1 (v/v) ratio as much as 1 ml at subcutaneous route. Booster was carried out 3 times with interval time of 14 days, using meat extract antigen emulsified in Freund's incomplete adjuvant at a 1:1 (v/v) ratio. Serum samples were taken every week, start from 1 week after the first immunization up to 1 week after the third booster. Antibody purification was performed using ammonium sulfate precipitation and Protein A. The presence of specific antibody was determined using agar gel precipitation test and enzyme-linked immunosorbent assay, while purified specific IgG was characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis method. Results: Specific antibody was detected at 14 days after the first immunization and still detected until 2 weeks after the third booster. Highest absorbance of specific antibody was detected 1 week after the third booster. Conclusion: The present study demonstrated that specific antibody of Sumateran wild boar is favorable to be produced in rabbit and showed that antibody produced is applicable to detect Sumateran wild boar meat antigen in immunodiffusion assay, indicating that it is promising as a reagent candidate in immunodiagnostic assay/kit.

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“…Gels were then removed from glass plates and were soaked in a coomassie brilliant blue dye solution for 3 h at room temperature with stirring. Methanol and acetic acid were used to remove the dye and separated protein bands were observed on clear gels [26].…”

Section: Methodsmentioning

confidence: 99%

Production of a polyclonal antibody against acrylamide for immunochromatographic detection of acrylamide using strip tests

Assaat¹,

Saepudin²,

Soejoedono³

et al. 2019

J Adv Vet Anim Res

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Objective:To produce, purify, and characterize a polyclonal antibody against acrylamide (anti-AA) for an application to immunochromatographic strip tests for AA.Materials and Methods:Polyclonal anti-AA was prepared by injecting N-acryloxysuccinimideconjugated bovine serum albumin hapten-antigen into New Zealand white rabbits. The antibody was purified using protein A, characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and conjugated with gold nanoparticles (AuNP). The conjugated antibody was then characterized using UV–Vis and FTIR spectroscopy and transmission electron microscopy (TEM). Immunochromatographic strip tests were performed using sample pads, conjugated pads, test zones, control zones, and absorbent pads. Strip tests were finally validated using standard AA solutions followed by the application of various concentrations of coffee samplesResults:Using SDS-PAGE, the purified anti-AA antibody was resolved at 50 and 25 kDa, indicating the presence of heavy and light chains, respectively. The conjugation of anti-AA with AuNP was confirmed using wavelength shifts in UV–Vis and FTIR spectra, and TEM analyses revealed increased diameters of AuNPs after conjugation. The immunochromatographic strip test was sensitive to 1 mgml-1 standard AA. Various concentrations of coffee samples resulted in red color differences in the test zone. High and low coffee concentrations produced thick and thin red lines, respectively.Conclusion:Purified anti-AA can be conjugated with AuNP to produce strip tests for detecting AA in coffee samples. The present immunochromatographic strip tests quantitatively showed increasing intensities of red lines with increasing AA concentrations.

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Production and characterization of Newcastle disease antibody as a reagent to develop a rapid immunodiagnostic test tool

Cited by 7 publications

References 32 publications

Production of hyperimmune serum against genotype VII Newcastle disease virus in rabbits with several applications

Production of hyperimmune serum against genotype VII Newcastle disease virus in rabbits with several applications

Sumateran wild boar (Sus scrofa vittatus) meat antibody production as immunodiagnostic reagent candidate

Production of a polyclonal antibody against acrylamide for immunochromatographic detection of acrylamide using strip tests

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