Patient Characteristics and Prevalence of Single Nucleotide Polymorphism (SNP) V16A MnSOD Gene in Diabetic Retinopathy Patients at RSUP Prof. Dr. I.G.N.G. Ngoerah Denpasar Bali

Abstract

Diabetic Retinopathy (DR) is a microvascular complication of Diabetes Mellitus (DM) caused by hyperglycemia and oxidative stress that is prevalent to the blindness risk. Manganese superoxide dismutase (MnSOD) gene affect the oxidative damage and progression of DR severity. This study aims to determine the association of patient characteristics on the severity and prevalence of Single Nucleotide Polymorphism (SNP) V16A in the MnSOD gene in DR patients at Prof. Dr. I.G.N.G. Ngoerah Denpasar Bali. Twelve (12) subjects with Non-Proliferative DR and 21 subjects with Proliferative DR were enrolled. Patient characteristics (age, T2DM duration, BMI, blood pressure) were associated to the DR severity using cross-sectional analytic study. While to detect the status of the SNP V16A MnSOD gene using descriptive experimental study. DNA segment amplification was performed using the PCR method, sequencing was performed to detect the base pattern at the location of the SNP. A domination of the VV genotype in the SNP V16A MnSOD gene was present in all subjects (100%). The majority of subjects were female (51,5%), age range 40-65 years (84,9%), T2DM duration <10 years (60,6%), normal BMI (60,6%). Blood pressure was dominated with non hypertension 60,6%, with a history of oral anti hypertensive. No significant association was found between patient characteristics and the DR severity (p<0,05). We may conclude that wildtype variant (VV) SNP V16A MnSOD gene was dominated in this finding. There is no significant association between patient characteristics and the severity of DR in Prof. Dr. I.G.N.G. Ngoerah Denpasar Bali. Keywords: Diabetic Retinopathy, MnSOD gene, SNP V16A