Patient-Specific Embryonic Stem Cells Derived From Human SCNT Blastocysts

Hwang, Woo Suk; Roh, Sangho; Lee, Byeong Chun; Kang, Sung Keun; Kwon, Dongil; Kim, Sue; Kim, Sun Jong; Park, Sun Woo; Kwon, Hyuksool; Lee, Chang Kyu; Lee, Jung Bok; Kim, Jin Mee; Ahn, Curie; Pack, Sun Ha; Chang, Sang Sik; Koo, Jung Jin; Yoon, Hyun Soo; Hwang, Jung Hye; Hwang, Youn Young; Park, Ye Soo; Oh, Sun Kyung; Kim, Hee Sun; Park, Jong Hyuk; Moon, Shin Yong; Schatten, Gerald

doi:10.1097/01.ogx.0000175803.94873.2b

Cited by 84 publications

(130 citation statements)

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“…Human ES cell lines H1 (NIH code WA01; passages [32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51] and H9 (NIH code WA09; passages 40 -44) were maintained in an undifferentiated state by weekly passages on mouse embryonic fibroblasts as previously described (25). A mouse bone marrow stromal cell line OP9 was obtained from Dr. T. Nakano (Research Institute for Microbial Diseases, Osaka University, Osaka, Japan).…”

Section: Cell Lines Cytokines and Mabsmentioning

confidence: 99%

Directed Differentiation of Human Embryonic Stem Cells into Functional Dendritic Cells through the Myeloid Pathway

Slukvin

Vodyanik

Thomson

et al. 2006

The Journal of Immunology

113

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We have established a system for directed differentiation of human embryonic stem (hES) cells into myeloid dendritic cells (DCs). As a first step, we induced hemopoietic differentiation by coculture of hES cells with OP9 stromal cells, and then, expanded myeloid cells with GM-CSF using a feeder-free culture system. Myeloid cells had a CD4+CD11b+CD11c+CD16+CD123lowHLA-DR− phenotype, expressed myeloperoxidase, and included a population of M-CSFR+ monocyte-lineage committed cells. Further culture of myeloid cells in serum-free medium with GM-CSF and IL-4 generated cells that had typical dendritic morphology; expressed high levels of MHC class I and II molecules, CD1a, CD11c, CD80, CD86, DC-SIGN, and CD40; and were capable of Ag processing, triggering naive T cells in MLR, and presenting Ags to specific T cell clones through the MHC class I pathway. Incubation of DCs with A23187 calcium ionophore for 48 h induced an expression of mature DC markers CD83 and fascin. The combination of GM-CSF with IL-4 provided the best conditions for DC differentiation. DCs obtained with GM-CSF and TNF-α coexpressed a high level of CD14, and had low stimulatory capacity in MLR. These data clearly demonstrate that hES cells can be used as a novel and unique source of hemopoietic and DC precursors as well as DCs at different stages of maturation to address essential questions of DC development and biology. In addition, because ES cells can be expanded without limit, they can be seen as a potential scalable source of cells for DC vaccines or DC-mediated induction of immune tolerance.

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Section: Cell Lines Cytokines and Mabsmentioning

confidence: 99%

Directed Differentiation of Human Embryonic Stem Cells into Functional Dendritic Cells through the Myeloid Pathway

Slukvin

Vodyanik

Thomson

et al. 2006

The Journal of Immunology

113

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“…Not all will be mentioned here, for example the papers by Hwang et al [19,20] because these results have been questioned in the literature (for example, see commentaries by David Cyranoski in Nature). It is difficult to draw firm conclusions from these papers.…”

Section: Inconsistent Resultsmentioning

confidence: 99%

The ups and downs of somatic cell nucleus transfer (SCNT) in humans

Fulka

Langerova

Loi

et al. 2013

J Assist Reprod Genet

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Achieving successful somatic cell nuclear transfer (SCNT) in the human and subhuman primate relative to other mammals has been questioned for a variety of technical and logistical issues. Here we summarize the gradual evolution of SCNT technology from the perspective of oocyte quality and cell cycle status that has recently led to the demonstration of feasibility in the human for deriving chromosomally normal stem cells lines. With these advances in hand, prospects for therapeutic cloning must be entertained in a conscientious, rigorous, and timely fashion before broad spectrum clinical applications are undertaken.Keywords Nucleus . Oocyte . Nucleus transfer Simply stated, SCNT in mammals should in practice be a very straightforward technique. First, cytoplasts are prepared by enucleating the metaphase II stage oocytes, i.e. metaphase II chromosomes are removed from the oocyte. Second, the selected nucleus is introduced into the cytoplast either by direct injection or by induced cell fusion. The reconstructed SCNT products are then activated in ways mimicking fertilization and if everything goes well cloned embryos develop [6].Logically, since following the birth of Dolly [7], and the production of additional clones in other mammalian species, there is ample confirmation that Dolly was not an exceptional case, prompting widespread discussion regarding the pros and cons of human SCNT. Given this prospect, it has been commonly accepted and broadly emphasized that the production of cloned human individuals must be banned (reproductive cloning). On the other hand, the production of embryos from which patient compatible embryonic stem cells could be obtained seemed to be acceptable, at least in some countries (therapeutic cloning). However, once the discovery of induced pluripotent stem cell production (iPSC) was realized by the work of Takahashi and Yamanaka [see 8 for review], illustrating that differentiated or even terminally differentiated cells can be converted (dedifferentiated) into a pluripotent state by induced overexpression of four factors (Oct 4, Sox 2, Klf 4, c-Myc; OSKM), greater resistance against the use of SCNT in humans has emerged, including the prospect of therapeutic cloning.Although many papers on modifications of the original iPS technology seeking improved efficiency and safety have been published, it should be emphasized that our understanding of the mechanisms by which iPSC elicits dedifferentiation remain woefully incomplete. Instead, it is commonly accepted that the oocyte cytoplasmic milieu is the most direct means to obtaining reprogramming of a differentiated somatic cell nucleus [9,10]. This fact-the quality of the oocyte cytoplasmic milieu-may well underscore the reason that while research on human SCNT continues, reports are rare in this species relative to the many papers published in other mammals. Birth of DollyThe work that led to the birth of Dolly was antedated by many years of experiments using laboratory and domestic animals to test many methodological combinations ...

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“…U maju iste godine je optuživan za etički prekršaj -korišćenje jajnih ćelija koje je pod pritiskom uzeo od svoje dve saradnice. Samo godinu dana kasnije objavio je rad o stvaranju 11 humanih embrionalnih matičnih ćelija [24]. Ovo je bilo veliko dostignuće u biotehnologiji, jer su ćelije navodno stvorene od somatskih ćelija pacijenata različitog uzrasta i pola.…”

Section: Fabrikovanje I Falsifikovanje Podatakaunclassified

Naučno nepoštenje Prevare – najteži prekršaji etike nauke

Bogdanović¹,

Dekić²

2016

Biomedicinska istraž.

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Kratak sadržajOsnovni postulat nauke je istinito, objektivno i potpuno prikazivanje rezultata istraživanja -naučno poštenje. Etički kodeks naučno-istraživačkog rada zvani dobra naučna praksa (DNP) naglašava da je pošten pristup istraživačkom procesu u svim njegovim fazama, kao i pošten odnos prema svim učesnicima u istraživanju -prema sebi, saradnicima i konkurentima -osnovni etički princip nauke, koji važi za sve naučne discipline, sve naučnike i sve nacije. Velika većina naučnika se pridržava ovog etičkog kodeksa, ali se povremeno otkrivaju nedopustive naučne prevare, od kojih su najteže fabrikovanje i falsifikovanje podataka i plagijatorstvo (FFP trijada). Kako ovakve prevare nanose ogromnu štetu ne samo instituciji u kojoj su se desile, nego i čitavoj naučnoj zajednici, u dokumentu DNP se objašnjavaju etiopatogeneza prevara, fenomenologija i načini sprečavanja ovakvih krajnje nepoželjnih i štetnih događaja. Sa ovim etičkim kodeksom treba da se upoznaju svi članovi akademske zajednice, a pridržavanje ovih principa je obavezujuće za svakog istraživača. U ovom članku ukazujemo na najnovije afere otkrivene u različitim oblastima nauke, kao i na mere prevencije i sankcionisanja naučnog nepoštenja.Ključne reči: prekršaji etike nauke, falsifikovanje, fabrikovanje, plagijatorstvo UvodPoslednjih nekoliko decenija zapaženo je da je naučno nepoštenje u porastu, ali se još uvek ne zna kolika je stvarna prevalencija prevara i obmana u nauci [1]. Takođe, nisu sasvim jasni ni razlozi porasta različitih vrsta prekršaja u nauci, a jedan od verovatnih razloga svakako može biti i efikasnije otkrivanje prevara. Podaci o naučnom nepoštenju u SAD se prikupljaju od 1990. godine i procenjuje se da je njihova učestalost 1/100.000 istraživača od ukupno 2 miliona aktivnih istraživača [2]. U istraživanju koje je sproveo British Medical Journal utvrđeno je da 6% od 2700 naučnika i lekara pribegava nekoj vrsti naučne prevare prilikom priprema radova i prezentacija [3]. U metaanalizi o učestalosti naučnog nepoštenja Fanelli [4] је saopštio da je 2% istraživača bar jedanput fabrikovalo, falsifikovalo ili modifikovalo

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Patient-Specific Embryonic Stem Cells Derived From Human SCNT Blastocysts

Cited by 84 publications

References 0 publications

Directed Differentiation of Human Embryonic Stem Cells into Functional Dendritic Cells through the Myeloid Pathway

Directed Differentiation of Human Embryonic Stem Cells into Functional Dendritic Cells through the Myeloid Pathway

The ups and downs of somatic cell nucleus transfer (SCNT) in humans

Naučno nepoštenje Prevare – najteži prekršaji etike nauke

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