Pattern formation of cytochrome c by microcontact printing and dip-pen nanolithography

Kwak, Sang Kyu; Lee, Gil Sun; Ahn, Dong June; Choi, Jeong Woo

doi:10.1016/j.msec.2003.09.015

Cited by 32 publications

(19 citation statements)

References 12 publications

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“…The size of the fusion protein dots was 2 lm in diameter and the spacing distance was 7 lm, which is in good agreement with the geometry of the original PDMS stamp having protruded dot regions of 3 lm in diameter and 10 lm in spacing [15]. After rinsing the substrate with DI-water, the average height of protein is slightly decreased since nonspecifically attached fusion protein molecules to the substrate were removed [15]. The patterns of fusion proteins obtained by micro contact printing method had high contrast and resolution.…”

Section: Verification Of Fusion Protein Sa Layersupporting

confidence: 80%

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Charge trap in self-assembled monolayer of cytochrome b562-green fluorescent protein chimera

Choi

Nam

Lee

et al. 2006

Current Applied Physics

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Section: Verification Of Fusion Protein Sa Layersupporting

confidence: 80%

“…After the stamp was displaced, substrate on which the pattern of fusion protein was formed was rinsed by DI water. This procedure is necessary to remove fusion protein non-specifically adsorbed by weak physical interactions [15]. Pattern formation was confirmed by STM measurement.…”

Section: Pattern Formation By Micro Contact Printing Methodsmentioning

confidence: 81%

Charge trap in self-assembled monolayer of cytochrome b562-green fluorescent protein chimera

Choi

Nam

Lee

et al. 2006

Current Applied Physics

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“…The reaction of IgG patterns with rabbit antibody and with mixtures of proteins was also studied using AFM to investigate and detect nonspecific binding to nanopatterns and to passivated areas of the substrate. In another study, Choi and coworkers immobilized cytochrome c through electrostatic adsorption on nanopatterns of MHA written by DPN [87]. The areas surrounding the MHA nanopatterns were passivated with octadecanethiol.…”

Section: Dpn Of Sams and Proteinsmentioning

confidence: 99%

Nanolithography: Towards Fabrication of Nanodevices for Life Sciences

Ngunjiri

Garno

2003

Nanotechnologies for the Life Sciences

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The sections in this article are Introduction: Engineering Surfaces at the Nanoscale Immobilization of Biomolecules for Surface Assays Strategies for Linking Proteins to Surfaces Electrostatic Immobilization Covalent Immobilization Molecular Recognition and Specific Interactions Nonspecific Physical Adsorption to Surfaces SAM Chemistry Methods for Nanolithography with Proteins Bias‐induced Nanolithography of SAMs Force‐induced Nanolithography of SAMs DPN of SAMs and Proteins Latex Particle Lithography with Proteins Detection of Protein Binding at the Nanoscale Future Directions Advantages of Nanoscale Detection Development of Cantilever Arrays Concluding Remarks

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“…Because amine groups are abundant in most proteins, covalent binding with amide bond formation is generally used in protein patterning. For noncovalent binding approaches, electrostatic interactions,30–34 biotin‐avidin coupling,35–38 and coordination chemistry38–42 have been mainly performed. Subcellular‐scaled patterns of protein adherent molecules can be stored for a certain time period until just before reaction with a target protein.…”

Section: Introductionmentioning

confidence: 99%

User‐Friendly Universal and Durable Subcellular‐Scaled Template for Protein Binding: Application to Single‐Cell Patterning

Jang

Collins²,

Nettikadan³

2013

Adv Funct Materials

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A new method for subcellular-sized protein patterning on a SiOx substrate is demonstrated by dip-pen nanolithography printed aldehyde-terminated alkylsilane template. The aldehyde-silane template is stable and durable; for example, subcellular scaled IgG protein array can be obtained using oneyear old aldehyde-silane template. Moreover, single cell patterning is successfully carried out by extracellular material (ECM) protein microarray and nanoarray fabricated on an aldehyde-silane template. With more than half of chance, single-or double-cells are successfully attached on fi bronectin protein nanoarrays in 21 × 21 μ m 2 (7 × 7 dot array) and 42 × 42 μ m 2 (14 × 14 dot array). The fi bronectin nanoarray with small area (21 × 21 μ m 2 ) shows the more rate of single cell attachment. Therefore, it is also demonstrated that cell patterning can be controlled by adjusting the nanostructure of ECM materials.

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Pattern formation of cytochrome c by microcontact printing and dip-pen nanolithography

Cited by 32 publications

References 12 publications

Charge trap in self-assembled monolayer of cytochrome b562-green fluorescent protein chimera

Charge trap in self-assembled monolayer of cytochrome b562-green fluorescent protein chimera

Nanolithography: Towards Fabrication of Nanodevices for Life Sciences

User‐Friendly Universal and Durable Subcellular‐Scaled Template for Protein Binding: Application to Single‐Cell Patterning

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