User‐Friendly Universal and Durable Subcellular‐Scaled Template for Protein Binding: Application to Single‐Cell Patterning

Abstract

A new method for subcellular-sized protein patterning on a SiOx substrate is demonstrated by dip-pen nanolithography printed aldehyde-terminated alkylsilane template. The aldehyde-silane template is stable and durable; for example, subcellular scaled IgG protein array can be obtained using oneyear old aldehyde-silane template. Moreover, single cell patterning is successfully carried out by extracellular material (ECM) protein microarray and nanoarray fabricated on an aldehyde-silane template. With more than half of chance, single-or double-cells are successfully attached on fi bronectin protein nanoarrays in 21 × 21 μ m 2 (7 × 7 dot array) and 42 × 42 μ m 2 (14 × 14 dot array). The fi bronectin nanoarray with small area (21 × 21 μ m 2 ) shows the more rate of single cell attachment. Therefore, it is also demonstrated that cell patterning can be controlled by adjusting the nanostructure of ECM materials.

Aluminum Hydroxide Nano- and Microstructures Fabricated Using Scanning Probe Lithography with KOH Ink

“…The diameter ( D ) and height ( h ) of the dot pattern were 12.1 ± 0.2 μm and 35.9 ± 6.2 nm, respectively. The diameter of the dot pattern in liquid-ink DPN printing can be controlled by reducing the volume of the ink loaded onto the tip probe using repeated cycles of brief contact with the substrate Figure b-III presents the relationship between the number of times the KOH-ink-loaded tip was placed in contact with the Au/Al substrate and the diameter of the resulting dot pattern.…”

Massively Parallel Patterning of Complex 2D and 3D Functional Polymer Brushes by Polymer Pen Lithography

“…Also, gradient structures can serve as model systems to understand the transport and motion of biomolecules on the surface. , We demonstrate herein that the usage of PPL-patterned PGMA brushes for the fabrication of DNA oligonucleotide microarrays and 3D gradient arrays of proteins over large areas. It is worth noting that once being made, PGMA brushes are highly stable at ambient conditions so that they are active even after storage for a few months, providing benefits for practical biochip applications …”

“…It is worth noting that once being made, PGMA brushes are highly stable at ambient conditions so that they are active even after storage for a few months, providing benefits for practical biochip applications. 52 For DNA oligonucleotide arrays (Figure 4A), we first fabricated PGMA brushes with EG 3 SAM background by PPL, using four basic pattern designs, namely triangle, square, ring, and cross, as shown in Figure 4B and E. The line width for the four patterns ranged from 1.7 to 2.4 μm and the height was controlled as 30, 40, 50, and 60 nm by tuning the feature distance.…”

Artificial Biosystems by Printing Biology

“…Tween-20 10 1 µm Protein immobilization by DDI [78] Oligonucleotides Trehalose Detection of fungal pathogen [137] PEG derivatives -10 0 µm ECM protein immobilization by chemisorption [261] Phospholipids --10 1 µm Phospholipid pattern functionalization with DNA Origami [262] Phospholipids -10 0 -10 1 µm Gradient patterns of phospholipids [135] Phospholipids -10 1 µm Cells recruiting by immobilized protein [136] Thiols -10 -1 -10 0 µm Diffraction Gratings Fabrication [263] Thiols -10 0 µm Patterning on gold surfaces [264] Proteins 10-50% w/v glycerol 10 1 µm Glucose oxidase monolayer at silicon dioxide surface [69] Proteins DMSO/glycerol (9:1) 10 2 µm DMSO-rich liquid compartments [187] Proteins 30% w/v glycerol 10 2 µm Glycerol-rich liquid compartments [188] Lipids 2-bis(10,12tricosadiynoyl)-sn-glycero-3-phosphocholine (DiynePC) Fluorophore (0.1 mM), and 5% (v/v) glycerine.…”