Pattern of CsICE1 expression under cold or drought treatment and functional verification through analysis of transgenic Arabidopsis

Abstract

ABSTRACT. CsICE1 is thought to be involved in hardiness resistance of tea plants. Using seedling cuttings of biennial Wuniuzao in this study, the pattern of CsICE1 expression under cold temperature (4°, -5°C), drought [20% polyethylene glycol 6000 (PEG-6000)], and plant hormone [200 mg/L abscisic acid (ABA), 1 mg/L brassinolide (BR)] treatment was studied by real-time quantitative PCR. Additionally, stress resistance, such as the freezing resistance of CsICE1, was studied using Arabidopsis lines transformed with sense or anti-sense CsICE1 via Agrobacterium tumefaciens infection. Our results showed that CsICE1 mRNA could be induced under -5°C, PEG, ABA, or BR treatment, although the pattern of expression differed for all treatments. Compared to wild type (WT) and anti-sense ICE1 transgenic lines, sense lines displayed higher relative germination rates under salt and drought stress. After freezing treatment, the sense transgenic lines overexpressing CsICE1 showed a higher survival rate, increased levels of proline, and decreased levels of malonaldehyde. Conversely, compared with WT, anti-sense ICE1 transgenic lines had lower proline levels and higher malonaldehyde levels under freezing conditions. Our study 11260 Z.T. Ding et al. ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 11259-11270 (2015) indicates that CsICE1 is an important anti-freezing gene and that overexpression of CsICE1 can improve cold resistance and enhance salt and drought tolerance of transgenic lines.

Genes underlying cold acclimation in the tea plant (<i>Camellia sinensis</i> (L.) Kuntze)

“…У чайного растения определено несколько ключевых генов, участвующих в процессах адаптации к низкотемпературному стрессу: CsICE1 (FE861156), CsCBF1 (EU563238), CsCBF2 (KC702795) (Wang et al, 2012;Yuan et al, 2013). При сравнении ответных реакций контрастных по устойчивости к холоду сортов чая была установлена экспрессия различных генов: CsCBF1 -у устой чивого к холоду сорта чая, CsCBF2 -у неустойчивого (Wang et al, 2012;Ding et al, 2015). При этом высокий уровень экспрессии гена CsCBF1 отмечен в результате подавления экспрессии CsCBF2, что свидетельствовало об антагонизме действия этих генов.…”

Genome-wide transcriptome profiling provides overwintering mechanism of Agropyron mongolicum

“…Arabidopsis los5 and los6 [ 11 ] mutants with a decreased ABA level are more sensitive to low temperatures than wild types. At present, the role of ABA in plant cold resistance has been confirmed in many kinds of plants [ 12 – 19 ]. Research on the effect of membrane phosphatidylglycerol fatty acids on plasma composition, as well as on the relationship with cold hardiness, in 74 plant species showed that plasma membrane unsaturated fatty acid has a great influence on cold hardiness.…”