Pattern of Immune Response to GP43 fromParacoccidioides brasiliensisin Susceptible and Resistant Mice Is Influenced by Antigen-Presenting Cells

Almeida, Sandro Rogério de; Moraes, Jane Zveiter de; Camargo, Zoilo Pires de; Gesztesi, J.-L.; Mariano, Mário; Lopes, José Daniel

doi:10.1006/cimm.1998.1388

Cited by 43 publications

(37 citation statements)

References 42 publications

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“…In experimental paracoccidioidomycosis in mice, a role for antigen-presenting cells (APC) and IL-10 has been reported (3,6). High levels of this cytokine were found in seven patients, and, to some extent, this can explain several findings and is consistent with the recent report of increased in vitro production of IL-10 by leukocytes of patients with active paracoccidioidomycosis (2).…”

supporting

confidence: 82%

High Serum Interleukin-10 and Tumor Necrosis Factor Alpha Levels in Chronic Paracoccidioidomycosis

Fornari¹,

Bava²,

Guereño³

et al. 2001

Clin Diagn Lab Immunol

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In patients with chronic paracoccidioidomycosis (n ‫؍‬ 10), levels of tumor necrosis factor alpha, interleukin-10, and interleukin-2 in serum, measured by enzyme-linked immunosorbent assay (in picograms per milliliter, as mean ؎ standard error of the mean), were higher than in normal controls (n ‫؍‬ 8): 186 ؎ 40 versus 40 ؎ 7 (P < 0.05), 203 ؎ 95 versus 20 ؎ 8 (P ‫؍‬ 0.001), and 96.3 ؎ 78.57 versus 1.19 ؎ 1.19 (P ‫؍‬ 0.045), respectively. Gamma interferon and interleukin-4 levels were similar in patients and controls.

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supporting

confidence: 82%

High Serum Interleukin-10 and Tumor Necrosis Factor Alpha Levels in Chronic Paracoccidioidomycosis

Fornari¹,

Bava²,

Guereño³

et al. 2001

Clin Diagn Lab Immunol

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“…The results showed that, in resistant mice, purified gp43 seemed to have been preferentially presented by macrophages and to have stimulated Th1 lymphokine production. On the other hand, in susceptible mice, gp43 was distinguishably presented by B cells, which led to stronger activation of Th2 subsets (33).…”

Section: Introductionmentioning

confidence: 99%

The low efficiency of dendritic cells and macrophages from mice susceptible to Paracoccidioides brasiliensis in inducing a Th1 response

Almeida

Lopes

2001

Braz J Med Biol Res

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In the present study we evaluated T cell proliferation and Th lymphokine patterns in response to gp43 from Paracoccidioides brasiliensis presented by isolated dendritic cells from susceptible and resistant mice. T cell proliferation assays showed that dendritic cells from susceptible mice were less efficient than those from resistant mice. The pattern of T cell lymphokines stimulated by dendritic cells was always Th1, although the levels of IL-2 and IFN-g were lower in T cell cultures from susceptible mice. To determie whether different antigen-presenting cells such as macrophages and dendritic cells stimulated different concentrations of Th1 lymphokines, the production of IFN-g and IL-2 was measured. It was observed that dendritic cells were more efficient than macrophages in stimulating lymphoproliferation in resistant mice. However, no significant difference was observed for IFN-g or IL-2 production. When cells from susceptible mice were used, macrophages were more efficient in stimulating lymphoproliferation than dendritic cells, but no difference was observed in the production of Th1 cytokine. Taken together, these results suggest the lower efficiency of dendritic cells and macrophages from B10.A mice in stimulating T cells that secrete Th1 lymphokines in vitro, an effect that may be involved in the progression of the disease in vivo.

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“…It is assumed that recovery was due to an adaptation of the animal's body to this kind of food, since functional foods have an action with continuous use in the diet. In this sense, we can also observe that the infection itself did not affect cell proliferation in the early phase of the infection, but in the 8 th week of the experiment, an increase in the proliferation of lymphocytes was seen, which may be due to the balance achieved between immune and inflammatory intermediaries not observed in susceptible animals with experimental PCM, as in the case of Swiss mice (Almeida et al, 1998).…”

Section: Resultsmentioning

confidence: 61%

<b>Influence of n-3 Polyunsaturated Fatty Acid in the Proliferative Activity of Lymphocytes During Experimental Infection with <i>Paracoccidioides brasiliensis

Navarini

Sargi²,

Visentainer

et al. 2018

Acta Sci. Health Sci.

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ABSTRACT. It has been shown that diets containing polyunsaturated omega-3 fatty acids may modulate the immune system. Paracoccidioidomycosis (PCM) is a systemic mycosis of great importance in Latin America, caused by the thermo-dimorphic fungus Paracoccidioides brasiliensis. The aim of this study was to evaluate the influence of n-3 PUFAs in lymphoproliferative activity in murine experimental PCM. Spleen lymphocytes from animals infected with an isolate of P. brasiliensis (Pb18) and fed with diets supplemented with flaxseed oil or a commercial diet for eight weeks, were obtained and cultured for evaluation of lymphoproliferative activity by MTT assay. In uninfected animals and those fed an enriched-flaxseed diet the evaluated lymphoproliferative activity was inhibited in the first week; however, this activity resumed at later stages. On the other hand, in animals infected and fed an enriched-flaxseed diet, the interaction between the stimulus given by the presence of the fungus and the action of n-3 PUFAs appeared to stimulate lymphocyte proliferation after the first week of infection. n-3 PUFAs are shown to influence lymphoproliferative activity of mice in experimental PCM.Keywords: omega-3, paracoccidioidomycosis, imune cell.Influência do ácido graxo poli-insaturado n-3 na atividade proliferativa de linfócitos durante a infecção com Paracoccidioides brasiliensis RESUMO. Tem-se demonstrado que dietas contendo ácido graxo poli-insaturado ômega-3 podem modular o sistema imune. A Paracoccidioidomicose é uma micose sistêmica de grande importância na América Latina, causada pelo fungo termo-dimórfico Paracoccidioides brasiliensis. O objetivo do estudo foi avaliar a influência do AGPI n-3 na atividade linfoproliferativa na PCM experimental murina. Linfócitos esplênicos de animais infectados com o isolado de P. brasiliensis e alimentados com uma dieta suplementada com óleo de linhaça por 8 semanas, foram obtidos e cultivados para avaliação da atividade linfoproliferativa através do ensaio de MTT. Nos animais não infectados e alimentados com a dieta enriquecida com linhaça houve a inibição da atividade linfoproliferativa na primeira semana, sendo retomada no estágio mais tardio. Por outro lado, nos animais infectados e suplementados com a dieta com óleo de linhaça, a interação entre o estímulo dado pela presença do fungo e a ação do AGPI n-3 parecem ter estimulado a proliferação de linfócitos depois da primeira semana de infecção. AGPI n-3 podem ter influenciado a atividade linfoproliferativa dos camundongos na PCM experimental.Palavras-chave: ômega-3, paracoccidioidomicose, células imune.

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Pattern of Immune Response to GP43 fromParacoccidioides brasiliensisin Susceptible and Resistant Mice Is Influenced by Antigen-Presenting Cells

Cited by 43 publications

References 42 publications

High Serum Interleukin-10 and Tumor Necrosis Factor Alpha Levels in Chronic Paracoccidioidomycosis

High Serum Interleukin-10 and Tumor Necrosis Factor Alpha Levels in Chronic Paracoccidioidomycosis

The low efficiency of dendritic cells and macrophages from mice susceptible to Paracoccidioides brasiliensis in inducing a Th1 response

<b>Influence of n-3 Polyunsaturated Fatty Acid in the Proliferative Activity of Lymphocytes During Experimental Infection with <i>Paracoccidioides brasiliensis

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